Research Kits

Rapid and efficient preparation of DNA, proteins, and chromatin is vital in performing downstream analyses. Our suite of sample preparation kits are suitable for nearly any workflow, whether it be isolating chromatin for ChIP-Seq or ChIP-PCR applications or preparing DNA from a variety of starting materials for Bisulfite-Seq or MS-PCR. Our sample preparation kits enable you to go directly from extraction to reaction, offering countless possibilities and flexibility in your experimental workflow. Our DNA, protein, and chromatin preparation kits are the starting point for endless research opportunities and downstream applications.

Next generation sequencing (NGS) is a method of non-Sanger-based high throughput DNA sequencing in which millions or even billions of DNA strands can be sequenced in parallel. Epigenetic researchers utilize NGS as an important tool to gain detailed and comprehensive insight into epigenetic modifications of genomes belonging to numerous species and from many different cell types. NGS minimizes the need for fragment-cloning methods often used in Sanger sequencing of genomes and yields significantly more throughput.

DNA methylation is an epigenetic modification where a methyl group is added to cytosine bases, typically at CpG sites, by DNA methyltransferases (DNMTs), influencing gene expression without altering the DNA sequence. This modification can be dynamically regulated, as ten-eleven translocation (TET) enzymes catalyze the oxidation of 5-methylcytosine (5-mC...

RNA methylation, such as N6-methyladenosine (m6A), is a key epigenetic modification where methyl groups are added to RNA nucleotides by RNA methyltransferases, like METTL3/14, influencing RNA stability, splicing, translation, and cellular function. This modification is reversible, as RNA demethylases, including FTO and ALKBH5...

Chromatin studies, including chromatin immunoprecipitation (ChIP) and chromatin accessibility assays, are essential for understanding how DNA is packaged and regulated within the nucleus. These techniques help identify protein-DNA interactions, protein post-translational modifications, and accessible regulatory regions that influence gene expr...

Histone modifications, such as methylation, acetylation, phosphorylation, citrullination, and sumoylation, are epigenetic marks that regulate chromatin structure and gene expression by influencing DNA accessibility. These modifications are catalyzed by specialized histone-modifying enzymes, incl...

CUT&LUNCH (Cleavage Under Target and Liberate Unique Nucleic Complex Homogeneously) RNA Immunoprecipitation (RIP) is an advanced profiling technique that enables high-resolution and highly sensitive mapping of RNA-bound proteins. The CUT&LUNCH RIP assay significantly lowers input needs, processing time, and background signal compared to conventional RIP, providing more reliable profiles. EpigenTek delivers all-in-one CU...

Histone acetylation and histone deacetylation involve the addition or removal of an acetyl group on lysine residues in the N-terminal tail and on the surface of the nucelosome core of histone proteins. Acetylated and deacetylated histones are considered epigenetic tags within chromatin by relaxing (euchromatin) or tightening (heterochromatin) chromatin structure, subsequently increasing or decreasing gene transcription levels.

EpiQuik™ nucleic acid damage assays are ELISA-based methods for quantifying global levels of 8-OHdG, 8-OhG, and other nucleic acid damage biomarkers from intact DNA/RNA. The EpiQuik™ technology allows for binding of sample DNA/RNA from any species directly to the assay plate wells, conveniently eliminating the need for nucleic acid denaturation/digestion and plate blocking steps. Assay accuracy...

EpiQuik™ CRISPR/Cas9 assays are ELISA-based methods for quantifying CRISPR-associated protein 9 (Cas9 and dCas9) from both purified nuclease and whole cell extract samples. The EpiQuik™ technology allows for rapid and efficient colorimetric detection of Cas9 of either S. pyogenes or S. aureus origin, conveniently eliminating the need for electrophoresis. EpigenTek delivers all-in-one resources to...

Various histone modifications, such as histone citrullination and histone phosphorylation, have been shown to epigenetically impact gene expression through different mechanisms. Adjusting epigenetic marks and changing chromatin structure – known as chromatin remodeling – as a result of these histone modifications can influence gene regulation in many tissue types. Studying these histone modifications provides researchers with valuable insight into cellular processes such as apoptosis, cel...

Testing is a crucial factor in being able to control and defeat the outbreak of COVID-19. At EpiGentek, we have utilized our resources to create low-cost COVID-19/SARS-CoV-2 testing kits for healthcare professionals and researchers. This collection of kits are designed to rapidly detect IgG and IgM antibodies of SARS-CoV-2 present in blood, serum or plasma.