DNA Methyltransferase & Demethylase Assays

DNA methyltransferases, or DNMTs, catalyze DNA methylation by adding methyl groups to the 5-carbon position of the cytosine ring, resulting in 5-methylcytosine. The various types of DNMTs are responsible for the maintenance and establishment of DNA methylation patterns. The ten-eleven translocation, or TET, family of 5-mC hydroxylase enzymes including TET1 are responsible for oxidizing 5-methylcytosine into 5-hydroxymethylcytosine. Measuring DNMT and TET amounts, activity, and inhibition levels will allow us to better understand the relationships between cytosine, 5-methylcytosine, and 5-hydroxymethylcytosine as part of the DNA methylation and DNA demethylation cycle.

	EpiQuik DNMT Activity/Inhibition Assay Ultra Kit (Colorimetric)Cat #P-3009	Epigenase 5mC-Hydroxylase TET Activity/Inhibition Assay Kit (Colorimetric) Cat #P-3086	Epigenase Thymine DNA Glycosylase (TDG) Activity/Inhibition Assay Kit (Colorimetric) Cat #P-3094	EpiQuik DNA Demethylase Activity/Inhibition Assay Ultra KitCat #P-3008
	More Details	More Details	More Details	More Details
Purpose	Colorimetrically measure total DNMT activity/inhibition	Colorimetrically measure total TET activity/inhibition	Colorimetrically measure total TDG activity/inhibition	Colorimetrically measure total DNA demethylase activity/inhibition
Starting Material	Nuclear extract or purified enzyme	Nuclear extract or purified enzyme	Nuclear extract or purified enzyme	Nuclear extract
Input Range	0.5-20 µg of nuclear extract; or 20-200 ng of purified enzyme	2-20 µg of nuclear extract; or 20-1000 ng of purified enzyme	2-10 µg of nuclear extract; or 20-1000 ng of purified enzyme	2-20 µg of nuclear extract
Total Time	4 hours	5 hours	5 hours	4 hours
Detection Threshold	0.5 ng of purified DNMT	20 ng of purified TET	20 ng of purified TDG	1 µg of nuclear extract
Positive Control Included
Format	ELISA-like Microplate-based	ELISA-like Microplate-based	ELISA-like Microplate-based	ELISA-like Microplate-based

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Displaying 1 to 15 (of 15 products)

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EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit

Citations (125)

Input Type:	DNA
Research Area:	DNA Methylation
Target Application:	Activity Measurement
Vessel Format:	96-Well Plate

P-3001-1	48 assays	$317.00
P-3001-2	96 assays	$534.00

Research Kits

EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit (Fluorometric)

Input Type:	DNA
Research Area:	DNA Methylation
Target Application:	Activity Measurement
Vessel Format:	96-Well Plate

P-3004-48	48 assays	$328.00
P-3004-96	96 assays	$544.00

Research Kits

EpiQuik DNMT1 Activity/Inhibitor Screening Assay Core Kit

Citations (1)

Input Type:	Purified Enzyme
Research Area:	DNA Methylation
Target Application:	Activity Measurement
Vessel Format:	96-Well Plate

P-3006A-48	48 assays	$256.00
P-3006A-96	96 assays	$483.00

Research Kits

EpiQuik DNMT3B Activity/Inhibitor Screening Assay Core Kit

Input Type:	Purified Enzyme
Research Area:	DNA Methylation
Target Application:	Activity Measurement
Vessel Format:	96-Well Plate

P-3007A-48	48 assays	$256.00
P-3007A-96	96 assays	$483.00

Research Kits

EpiQuik DNA Demethylase Activity/Inhibition Assay Ultra Kit

Citations (2)

Input Type:	Nuclear Extracts, Purified Enzyme
Research Area:	DNA Methylation
Target Application:	Activity Measurement
Vessel Format:	96-Well Plate

P-3008-48	48 assays	$349.00
P-3008-96	96 assays	$524.00

Research Kits

EpiQuik DNMT Activity/Inhibition Assay Ultra Kit (Colorimetric)

Citations (48) | (1)

Input Type:	Nuclear Extracts, Purified Enzyme
Research Area:	DNA Methylation
Target Application:	Activity Measurement
Vessel Format:	96-Well Plate

P-3009-48	48 assays	$327.00
P-3009-96	96 assays	$551.00

Research Kits

EpiQuik DNMT Activity/Inhibition Assay Ultra Kit (Fluorometric)

Citations (7)

Input Type:	Nuclear Extracts, Purified Enzyme
Research Area:	DNA Methylation
Target Application:	Activity Measurement
Vessel Format:	96-Well Plate

P-3010-48	48 assays	$338.00
P-3010-96	96 assays	$549.00

Research Kits

EpiQuik Dnmt1 Assay Kit

Citations (21)

Input Type:	Nuclear Extracts, Purified Enzyme
Research Area:	DNA Methylation
Target Application:	Amount Quantitation
Vessel Format:	96-Well Plate

P-3011-2	48 assays	$399.00
P-3011-3	96 assays	$549.00

Research Kits

EpiQuik Dnmt3A Assay Kit

Citations (4)

Input Type:	Nuclear Extracts, Purified Enzyme
Research Area:	DNA Methylation
Target Application:	Amount Quantitation
Vessel Format:	96-Well Plate

P-3012-2	48 assays	$399.00
P-3012-3	96 assays	$549.00

Research Kits

EpiQuik Dnmt3B Assay Kit

Citations (7)

Input Type:	Nuclear Extracts, Purified Enzyme
Research Area:	DNA Methylation
Target Application:	Amount Quantitation
Vessel Format:	96-Well Plate

P-3013-2	48 assays	$399.00
P-3013-3	96 assays	$549.00

Research Kits

EpiQuik DNA Demethylase Activity/Inhibition Assay Kit

Citations (1)

Input Type:	Nuclear Extracts, Purified Enzyme
Research Area:	DNA Methylation
Target Application:	Activity Measurement
Vessel Format:	96-Well Plate

P-3019-48	48 assays	$369.00
P-3019-96	96 assays	$549.00

Research Kits

Epigenase 5mC-Hydroxylase TET Activity/Inhibition Assay Kit (Colorimetric)

Citations (12)

Input Type:	Nuclear Extracts, Purified Enzyme
Research Area:	DNA Methylation
Target Application:	Activity Measurement
Vessel Format:	96-Well Plate

P-3086-48	48 assays	$317.00
P-3086-96	96 assays	$539.00

Research Kits

Epigenase 5mC-Hydroxylase TET Activity/Inhibition Assay Kit (Fluorometric)

Citations (3)

Input Type:	Nuclear Extracts, Purified Enzyme
Research Area:	DNA Methylation
Target Application:	Activity Measurement
Vessel Format:	96-Well Plate

P-3087-48	48 assays	$317.00
P-3087-96	96 assays	$539.00

Research Kits

Epigenase Thymine DNA Glycosylase (TDG) Activity/Inhibition Assay Kit (Colorimetric)

Citations (2)

Input Type:	Nuclear Extracts, Purified Enzyme
Research Area:	DNA Methylation
Target Application:	Activity Measurement
Vessel Format:	96-Well Plate

P-3094-48	48 Assays	$328.00
P-3094-96	96 Assays	$559.00

Research Kits

Epigenase m6A Demethylase Activity/Inhibition Assay Kit (Colorimetric) new product

Input Type:	Nuclear Extracts or Purified Enzymes
Research Area:	RNA Methylation
Target Application:	Amount Quantitation
Vessel Format:	96-Well Plate

P-9013-48	48 assays	$329.00
P-9013-96	96 assays	$529.00

Research Kits

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Displaying 1 to 15 (of 15 products)

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DNA Methyltransferase & Demethylase Assays

EpiQuik DNMT Activity/Inhibition Assay Ultra Kit (Colorimetric)Cat #P-3009

Epigenase 5mC-Hydroxylase TET Activity/Inhibition Assay Kit (Colorimetric) Cat #P-3086

Epigenase Thymine DNA Glycosylase (TDG) Activity/Inhibition Assay Kit (Colorimetric) Cat #P-3094

EpiQuik DNA Demethylase Activity/Inhibition Assay Ultra KitCat #P-3008