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   Home  »  Research Kits  »  DNA Methylation  »  DNA Methyltransferase & Demethylase Assays 
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DNA Methyltransferase & Demethylase Assays

DNA methyltransferases, or DNMTs, catalyze DNA methylation by adding methyl groups to the 5-carbon position of the cytosine ring, resulting in 5-methylcytosine. The various types of DNMTs are responsible for the maintenance and establishment of DNA methylation patterns. The ten-eleven translocation, or TET, family of 5-mC hydroxylase enzymes including TET1 are responsible for oxidizing 5-methylcytosine into 5-hydroxymethylcytosine. Measuring DNMT and TET amounts, activity, and inhibition levels will allow us to better understand the relationships between cytosine, 5-methylcytosine, and 5-hydroxymethylcytosine as part of the DNA methylation and DNA demethylation cycle. This in turn can help us start to better understand and use these findings to find and fight epigenetic diseases.

 

EpiQuik DNMT Activity/Inhibition Assay Ultra Kit (Colorimetric)Cat #P-3009

Epigenase 5mC-Hydroxylase TET Activity/Inhibition Assay Kit (Colorimetric) Cat #P-3086

Epigenase Thymine DNA Glycosylase (TDG) Activity/Inhibition Assay Kit (Colorimetric) Cat #P-3094

EpiQuik DNA Demethylase Activity/Inhibition Assay Ultra KitCat #P-3008

  More DetailsMore DetailsMore DetailsMore Details
PurposeColorimetrically measure total DNMT activity/inhibitionColorimetrically measure total TET activity/inhibitionColorimetrically measure total TDG activity/inhibitionColorimetrically measure total DNA demethylase activity/inhibition
Starting MaterialNuclear extract or purified enzymeNuclear extract or purified enzymeNuclear extract or purified enzymeNuclear extract
Input Range0.5-20 µg of nuclear extract; or 20-200 ng of purified enzyme2-20 µg of nuclear extract; or 20-1000 ng of purified enzyme2-10 µg of nuclear extract; or 20-1000 ng of purified enzyme2-20 µg of nuclear extract
Total Time4 hours5 hours5 hours4 hours
Detection Threshold0.5 ng of purified DNMT20 ng of purified TET20 ng of purified TDG1 µg of nuclear extract
Positive Control Included
FormatELISA-like Microplate-basedELISA-like Microplate-basedELISA-like Microplate-basedELISA-like Microplate-based
Result Pages:  1  Displaying 1 to 16 (of 16 products)
Image Description Cat. No. Size Price
EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit
EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit

Citations (147)
Input Type: DNA
Research Area: DNA Methylation
Target Application: Activity Measurement
Vessel Format: 96-Well Plate
P-3001-148 assays $353.00 Add to Cart
P-3001-296 assays $593.00 Add to Cart
Research Kits
EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit (Fluorometric)
EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit (Fluorometric)

Input Type: DNA
Research Area: DNA Methylation
Target Application: Activity Measurement
Vessel Format: 96-Well Plate
P-3004-4848 assays $364.00 Add to Cart
P-3004-9696 assays $603.00 Add to Cart
Research Kits
EpiQuik DNMT1 Activity/Inhibitor Screening Assay Core Kit
EpiQuik DNMT1 Activity/Inhibitor Screening Assay Core Kit

Citations (8)
Input Type: Purified Enzyme
Research Area: DNA Methylation
Target Application: Activity Measurement
Vessel Format: 96-Well Plate
P-3006A-4848 assays $283.00 Add to Cart
P-3006A-9696 assays $531.00 Add to Cart
Research Kits
EpiQuik DNMT3B Activity/Inhibitor Screening Assay Core Kit
EpiQuik DNMT3B Activity/Inhibitor Screening Assay Core Kit

Citations (5)
Input Type: Purified Enzyme
Research Area: DNA Methylation
Target Application: Activity Measurement
Vessel Format: 96-Well Plate
P-3007A-4848 assays $283.00 Add to Cart
P-3007A-9696 assays $531.00 Add to Cart
Research Kits
EpiQuik DNA Demethylase Activity/Inhibition Assay Ultra Kit
EpiQuik DNA Demethylase Activity/Inhibition Assay Ultra Kit

Citations (3)
Input Type: Nuclear Extracts, Purified Enzyme
Research Area: DNA Methylation
Target Application: Activity Measurement
Vessel Format: 96-Well Plate
P-3008-4848 assays $385.00 Add to Cart
P-3008-9696 assays $583.00 Add to Cart
Research Kits
EpiQuik DNMT Activity/Inhibition Assay Ultra Kit (Colorimetric)
EpiQuik DNMT Activity/Inhibition Assay Ultra Kit (Colorimetric)

Citations (120) | (3)
Input Type: Nuclear Extracts, Purified Enzyme
Research Area: DNA Methylation
Target Application: Activity Measurement
Vessel Format: 96-Well Plate
P-3009-4848 assays $364.00 Add to Cart
P-3009-9696 assays $603.00 Add to Cart
Research Kits
EpiQuik DNMT Activity/Inhibition Assay Ultra Kit (Fluorometric)
EpiQuik DNMT Activity/Inhibition Assay Ultra Kit (Fluorometric)

Citations (8)
Input Type: Nuclear Extracts, Purified Enzyme
Research Area: DNA Methylation
Target Application: Activity Measurement
Vessel Format: 96-Well Plate
P-3010-4848 assays $375.00 Add to Cart
P-3010-9696 assays $603.00 Add to Cart
Research Kits
EpiQuik Dnmt1 Assay Kit
EpiQuik Dnmt1 Assay Kit

Citations (35)
Input Type: Nuclear Extracts, Purified Enzyme
Research Area: DNA Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
P-3011-248 assays $427.00 Add to Cart
P-3011-396 assays $603.00 Add to Cart
Research Kits
EpiQuik Dnmt3A Assay Kit
EpiQuik Dnmt3A Assay Kit

Citations (10)
Input Type: Nuclear Extracts, Purified Enzyme
Research Area: DNA Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
P-3012-248 assays $427.00 Add to Cart
P-3012-396 assays $603.00 Add to Cart
Research Kits
EpiQuik Dnmt3B Assay Kit
EpiQuik Dnmt3B Assay Kit

Citations (10)
Input Type: Nuclear Extracts, Purified Enzyme
Research Area: DNA Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
P-3013-248 assays $427.00 Add to Cart
P-3013-396 assays $603.00 Add to Cart
Research Kits
EpiQuik DNA Demethylase Activity/Inhibition Assay Kit
EpiQuik DNA Demethylase Activity/Inhibition Assay Kit

Citations (2)
Input Type: Nuclear Extracts, Purified Enzyme
Research Area: DNA Methylation
Target Application: Activity Measurement
Vessel Format: 96-Well Plate
P-3019-4848 assays $405.00 Add to Cart
P-3019-9696 assays $603.00 Add to Cart
Research Kits
Epigenase 5mC-Hydroxylase TET Activity/Inhibition Assay Kit (Colorimetric)
Epigenase 5mC-Hydroxylase TET Activity/Inhibition Assay Kit (Colorimetric)

Citations (34)
Input Type: Nuclear Extracts, Purified Enzyme
Research Area: DNA Methylation
Target Application: Activity Measurement
Vessel Format: 96-Well Plate
P-3086-4848 assays $353.00 Add to Cart
P-3086-9696 assays $593.00 Add to Cart
Research Kits
Epigenase 5mC-Hydroxylase TET Activity/Inhibition Assay Kit (Fluorometric)
Epigenase 5mC-Hydroxylase TET Activity/Inhibition Assay Kit (Fluorometric)

Citations (12)
Input Type: Nuclear Extracts, Purified Enzyme
Research Area: DNA Methylation
Target Application: Activity Measurement
Vessel Format: 96-Well Plate
P-3087-4848 assays $353.00 Add to Cart
P-3087-9696 assays $593.00 Add to Cart
Research Kits
Epigenase Thymine DNA Glycosylase (TDG) Activity/Inhibition Assay Kit (Colorimetric)
Epigenase Thymine DNA Glycosylase (TDG) Activity/Inhibition Assay Kit (Colorimetric)

Citations (2)
Input Type: Nuclear Extracts, Purified Enzyme
Research Area: DNA Methylation
Target Application: Activity Measurement
Vessel Format: 96-Well Plate
P-3094-4848 Assays $364.00 Add to Cart
P-3094-9696 Assays $614.00 Add to Cart
Research Kits
EpiQuik DNMT Activity/Inhibition ELISA Easy Kit (Colorimetric)
EpiQuik DNMT Activity/Inhibition ELISA Easy Kit (Colorimetric)

Citations (1) | (1)
Input Type: Nuclear Extracts, Purified Enzyme
Research Area: DNA Methylation
Target Application: Activity Measurement
Vessel Format: 96-Well Plate
P-3139-4848 assays $356.00 Add to Cart
P-3139-9696 assays $591.00 Add to Cart
Research Kits
Epigenase m6A Demethylase Activity/Inhibition Assay Kit (Colorimetric)
Epigenase m6A Demethylase Activity/Inhibition Assay Kit (Colorimetric)

Citations (2)
Input Type: Nuclear Extracts, Purified Enzymes
Research Area: RNA Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
P-9013-4848 assays $353.00 Add to Cart
P-9013-9696 assays $561.00 Add to Cart
Research Kits
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