DNA Methyltransferase & Demethylase Assays

DNA methyltransferases, or DNMTs, catalyze DNA methylation by adding methyl groups to the 5-carbon position of the cytosine ring, resulting in 5-methylcytosine. The various types of DNMTs are responsible for the maintenance and establishment of DNA methylation patterns. The ten-eleven translocation, or TET, family of 5-mC hydroxylase enzymes including TET1 are responsible for oxidizing 5-methylcytosine into 5-hydroxymethylcytosine. Measuring DNMT and TET amounts, activity, and inhibition levels will allow us to better understand the relationships between cytosine, 5-methylcytosine, and 5-hydroxymethylcytosine as part of the DNA methylation and DNA demethylation cycle. This in turn can help us start to better understand and use these findings to find and fight epigenetic diseases.

	EpiQuik DNMT Activity/Inhibition Assay Ultra Kit (Colorimetric)Cat #P-3009	Epigenase 5mC-Hydroxylase TET Activity/Inhibition Assay Kit (Colorimetric) Cat #P-3086	Epigenase Thymine DNA Glycosylase (TDG) Activity/Inhibition Assay Kit (Colorimetric) Cat #P-3094	EpiQuik DNA Demethylase Activity/Inhibition Assay Ultra KitCat #P-3008
	More Details	More Details	More Details	More Details
Purpose	Colorimetrically measure total DNMT activity/inhibition	Colorimetrically measure total TET activity/inhibition	Colorimetrically measure total TDG activity/inhibition	Colorimetrically measure total DNA demethylase activity/inhibition
Starting Material	Nuclear extract or purified enzyme	Nuclear extract or purified enzyme	Nuclear extract or purified enzyme	Nuclear extract
Input Range	0.5-20 µg of nuclear extract; or 20-200 ng of purified enzyme	2-20 µg of nuclear extract; or 20-1000 ng of purified enzyme	2-10 µg of nuclear extract; or 20-1000 ng of purified enzyme	2-20 µg of nuclear extract
Total Time	4 hours	5 hours	5 hours	4 hours
Detection Threshold	0.5 ng of purified DNMT	20 ng of purified TET	20 ng of purified TDG	1 µg of nuclear extract
Positive Control Included
Format	ELISA-like Microplate-based	ELISA-like Microplate-based	ELISA-like Microplate-based	ELISA-like Microplate-based