Browse practical epigenetics articles, application notes, and research resources organized by topic. Use search to quickly locate guidance relevant to your workflow.
A practical guide to using global m6A measurements as a starting point, then choosing enrichment, sequencing, and validation strategies that connect RNA methylation changes to specific transcripts and...
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Learn how m6A RNA methylation regulates tumor immunity, checkpoint response, and therapeutic resistance, and how to choose assays for global m6A quantification, MeRIP enrichment, and m6A-seq.
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This comprehensive analysis highlights the critical roles of methyltransferases, demethylases, and binding proteins in RNA modification, along with their implications in physiology and disease.
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Often referred to as “the fifth RNA base”, N6-methyladenosine, or m6A, is the most common and abundant eukaryotic RNA modification, making it the primary target of study in epitranscriptomic research. The...
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RNA plays a vital role in many biological processes. Preparing isolated and purified RNA from biological samples such as cells and tissues is thus a critical step in many scientific experiments, including...
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N6-Methyladenosine, or m6A, is formed when a methyl group is chemically added at the nitrogen-6 position of adenosine (A) residues. Often referred to as “the fifth RNA base”, m6A is the most common and...
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Pulmonary hypertension (PH) is a type of high blood pressure that affects the blood vessels in the lungs, as well as the right side of the heart. Arteries in the lung become damaged as a result of an imbalance...
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RNA methylation is a reversible chemical modification involved in the epigenetic regulation of numerous biological processes. It occurs in different RNA types (tRNA, rRNA, mRNA, tmRNA, snRNA, snoRNA, miRNA,...
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Related Protocols:Ultracentrifugal RNA Virus Purification | RNA Virus Amplification in Cell Culture | Phenol/Chloroform RNA Extraction | Concentrating RNA Viruses by PEG Precipitation
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Related Protocols:Ultracentrifugal RNA Virus Purification | RNA Virus Amplification in Cell Culture | Phenol/Chloroform RNA Extraction | Concentrating RNA Viruses by PEG Precipitation
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The following protocol has been established for rotavirus purification, but may also be applicable for other RNA viruses . A floor-type ultracentrifuge such as Beckman Coulter's Optima ultracentrifuge is...
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Related Protocols: Ultracentrifugal RNA Virus Purification | RNA Virus Amplification in Cell Culture | Phenol/Chloroform RNA Extraction | Concentrating RNA Viruses by PEG Precipitation
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As the COVID-19 pandemic continues to disrupt the quality of life, investigators are gaining more insight into the SARS-CoV-2 virus behind this disease. Continued research in elucidating the dynamics of the...
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We look into several main factors that should be taken into account when extracting RNA to help maximize the amount of resulting RNA and its purity.
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Conrad H. Waddington, a British scientist with an eclectic background in developmental biology, paleontology, genetics, and embryology, first coined the term “epigenetics” in 1942 to describe the...
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