Chromatin Immunoprecipitation

Chromatin immunoprecipitation (ChIP) is a laboratory technique used to study the interaction between proteins and DNA in a specific region of the genome. It allows researchers to identify which proteins bind to specific DNA sequences and to determine the relative strength of these interactions.

The basic principle of ChIP is to isolate chromatin (the complex of DNA and proteins that make up the chromosomes in cells) from cells or tissue, and then use an antibody specific to a particular protein of interest to pull down (precipitate) the DNA sequences that are bound to that protein. The precipitated DNA sequences can then be analyzed using techniques such as polymerase chain reaction (PCR) or DNA sequencing to identify the specific DNA sequences that are bound to the protein of interest.

ChIP is a powerful tool for studying gene regulation and has been widely used to identify regulatory elements in the genome, including promoters, enhancers, and other regulatory elements that control gene expression. It has also been used to study the role of specific proteins in various biological processes, including development, differentiation, and disease.

	ChromaFlash High Sensitivity ChIP Kit Cat #P-2027	EpiQuik ChIP Kit Cat #P-2002	EpiQuik Tissue ChIP Kit Cat #P-2003	EpiQuik Plant ChIP Kit Cat #P-2014	ChromaFlash One-Step Magnetic ChIP Kit Cat #P-2026
Purpose	Selective immunoprecipitation and enrichment of chromatin fractions from low amount of cells or tissue	Selective immunoprecipitation and enrichment of chromatin fractions from cells	Selective immunoprecipitation and enrichment of chromatin fractions from tissue	Selective immunoprecipitation and enrichment of chromatin fractions from plants	Immunoprecipitate and shear isolated chromatin using magnetic beads in one step
Key Points	Recommended for next-gen sequencing applications	Robust protocol designed specifically for cell input	Robust protocol designed specifically for tissue input	Robust protocol designed specifically for plant tissue input	Magnetic beads-based ChIP allows for automation
Starting Material	Cells or Tissue	Cells	Tissue	Plant Tissue (flowers, leaves, young seedlings)	Isolated Chromatin
Input Range	2,000 to 1,000,000 cells or 0.5 to 50 mg tissue per reaction	500,000 to 2,000,000 cells per reaction	10 to 30 mg tissue per reaction	20 to 50 mg tissue per reaction	0.1 to 15 µg chromatin per reaction
Total Protocol Time	5 hours	5 hours	5 hours	6 hours	4 hours
Relative-Fold Enrichment vs IgG	500+	100 to 200	100 to 200	100 to 200	100 to 200
DNA Purification Columns Included					Not needed
Negative Control Included
Control Primers Included				Not needed
Vessel Format	Strip-Well Microplate	Strip-Well Microplate	Strip-Well Microplate	Strip-Well Microplate	Strip-Well Microplate
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