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EpiQuik Tissue Acetyl-Histone H4 ChIP Kit


For immunoprecipitating H4ac chromatin from tissue input samples via microplate format

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Schematic procedure of the EpiQuikâ„¢ Tissue Acetyl-Histone H4 ChIP Kit.
Input Type: Chromatin
Research Area: Chromatin & Transcription
Target Application: Immunoprecipitation
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-2013-2424 reactions $359.00 
P-2013-4848 reactions $569.00 
Order now & get it by Tuesday, November 26th  
Product Overview

The EpiQuik™ Tissue Acetyl-Histone H4 ChIP Kit is a convenient package of tools that allows the experimenter to investigate interactions of histone H4 acetylation and DNA in variety of mammalian tissues. The entire procedure can be completed within 5 hours and produces far superior results than any competitor kits. The EpiQuik™ Tissue Acetyl-Histone H4 ChIP kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, ChIP-Seq, and ChIP-on-chip. This kit has the following advantages: 

  • The fastest procedure available, which can be finished within 5 hours.
  • Strip microwell format makes the assay flexible: manual or high throughput.
  • Columns for DNA purification are included: save time and reduce labor.
  • Compatible with all DNA amplification-based approaches.
  • Simple, reliable, and consistent assay conditions.

Background Information  
Histone acetylation takes place at the α-amino groups of conserved lysine residues, where histone acetyltransferases (HATs) are responsible for catalyzing this modification. It has been demonstrated that histone H4 acetylation serves as an epigenetic marker of chromosomal domains. Histone H4 acetylation regulates various cellular physiological processes, including transcriptional activation of genes, chromatin assembly, cell proliferation, and some pathological processes such as tumorigenesis. Chromatin Immunoprecipitation (ChIP) is a powerful technique for studying protein-DNA interaction in vivo. ChIP also offers an advantageous tool that allows identification of activated genes associated with acetylated histone H4. ChIP coupled with microarrays could be further used for profiling or mapping histone H4 acetylation patterns. 

Principle & Procedure
This ChIP kit contains all reagents required for carrying out a successful chromatin immunoprecipitation for acetyl-histone H4 from mammalian cells. Particularly, this kit includes a ChIP-grade acetyl-histone H4 antibody and a negative control normal mouse IgG. Chromatin from the cells is extracted, sheared and added into the microwell immobilized with the antibody. DNA is released from the antibody-captured acetyl-histone H4 protein-DNA complex, reversed and purified through the specifically designed F-Spin Column. Eluted DNA can be used for various down-stream applications.

Starting Materials
Starting materials can include various tissue samples. In general, the input amount should be 20 mg of tissue for each reaction.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Safety Data Sheet]
BisulPlus Loci 5mC & 5hmC Detection PCR kit
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