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Introduction to Epigenetics

RNA Methylation

RNA methylation is a reversible post-translational modification to RNA that epigenetically impacts numerous biological processes. It occurs in different RNAs including tRNA, rRNA, mRNA, tmRNA, snRNA, snoRNA, miRNA, and viral RNA. Different catalytic strategies are employed for RNA methylation by a variety of RNA-methyltransferases.

N6-methyladenosine (m6A) is the most common and abundant methylation modification in RNA molecules present in eukaryotes and accounts for more than 80% of all RNA methylation. It may be referred to as the “fifth RNA base” and has broad roles in regulating embryonic development and cell fates. In particular, this RNA modification is thought to be important for mRNA regulation due to enrichment of m6A at 3’UTRs. It has also been linked to cancers including leukemia, breast cancer, and prostate cancer.

m6A
N6-methyladenosine (m6A)
5-methylcytosine (5-mC) is an epigenetic mark that also commonly occurs in various RNA molecules. Researchers are able to bisulfite convert RNA, perform RT-PCR amplification, cloning, and sequencing to gain information about RNA cytosine methylation states. By treating RNA with bisulfite, cytosine residues are deaminated to uracil while leaving 5-methylcytosine intact.

Recent data strongly suggest that m6A and 5-mC RNA methylation affects the regulation of various biological processes such as RNA stability and mRNA translation, and that abnormal RNA methylation contributes to etiology of human diseases. Furthermore, loss of 5-mC in vault RNAs causes aberrant processing into Argonaute-associated small RNA fragments that can function as microRNAs. Researchers continue to investigate m6A and 5-mC in RNA to uncover the mysteries of RNA methylation.


 

m6A RNA Methylation Quantification KitCat #P-9005

5-mC RNA Methylation ELISA Easy Kit Cat #P-9009

RNA Bisulfite Conversion KitCat #P-9003

  Citations Citations Citations
Purpose An ELISA-like format for absorbance-based quantitation of N6-methyladenosine in RNA An ELISA-like format for fluorescence-based quantitation of 5-mC RNA methylation For robust, chemical-based bisulfite treatment of RNA for analysis of methylated cytosines
Key Points
  • Detect m6A RNA methylation in any species
  • Colorimetric ELISA-like assay using an m6A antibody
  • Detection limit as low as 10 pg of m6A
  • Large signal window, low background noise
  • Detect 5-mC RNA methylation in any species
  • High specificity to 5-mC, with no cross-reactivity
  • Input as low as 5 ng per reaction
  • >90% of RNA loss prevented
  • Completely converts unmethylated RNA cytosine into uracil 
Starting Material Total RNA Total RNATotal RNA
Lowest Input Amount50 pg 50-300 ng 200-500 ng
Total Protocol Time < 4 hours < 3 hours 3 hours
Reactions 48,96 48,96 50
Vessel Format 96-Well Plate96-Well PlateSpin Column
Result Pages:  1  Displaying 1 to 8 (of 8 products)
Image Description Cat. No. Size Price
Methylamp RNA Bisulfite Conversion Kit
Methylamp RNA Bisulfite Conversion Kit

Citations (1)
Input Type: RNA
Research Area: RNA Methylation
Target Application: Sample Modification
Vessel Format: Columns/Tubes
P-9003-05050 reactions $173.00 Add to Cart
Research Kits
EpiNext Hi-Fi cDNA Synthesis Kit
EpiNext Hi-Fi cDNA Synthesis Kit

Input Type: RNA
Research Area: RNA Methylation
Target Application: Library Construction, PCR Amplification, Sample Modification
Vessel Format: Columns/Tubes
P-9004-2020 samples $185.00 Add to Cart
Research Kits
EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)
EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

Citations (49) | (7)
Input Type: RNA
Research Area: RNA Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
P-9005-4848 reactions $308.00 Add to Cart
P-9005-9696 reactions $509.00 Add to Cart
Research Kits
EpiNext 5-mC RNA Bisulfite-Seq Easy Kit (Illumina)
EpiNext 5-mC RNA Bisulfite-Seq Easy Kit (Illumina)

Input Type: RNA
Research Area: RNA Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
P-9007-1212 reactions $509.00 Add to Cart
P-9007-2424 reactions $999.00 Add to Cart
Research Kits
EpiQuik m6A RNA Methylation Quantification Kit (Fluorometric)
EpiQuik m6A RNA Methylation Quantification Kit (Fluorometric)

Citations (1)
Input Type: RNA
Research Area: RNA Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
P-9008-4848 assays $308.00 Add to Cart
P-9008-9696 assays $509.00 Add to Cart
Research Kits
MethylFlash 5-mC RNA Methylation ELISA Easy Kit (Fluorometric)
MethylFlash 5-mC RNA Methylation ELISA Easy Kit (Fluorometric)

Citations (2)
Input Type: RNA
Research Area: RNA Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
P-9009-4848 reactions $308.00 Add to Cart
P-9009-9696 reactions $509.00 Add to Cart
Research Kits
Epigenase m6A Demethylase Activity/Inhibition Assay Kit (Colorimetric)
Epigenase m6A Demethylase Activity/Inhibition Assay Kit (Colorimetric)

Citations (1)
Input Type: Nuclear Extracts or Purified Enzymes
Research Area: RNA Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
P-9013-4848 assays $339.00 Add to Cart
P-9013-9696 assays $539.00 Add to Cart
Research Kits
MethylFlash Urine N6-methyladenosine (m6A) Quantification Kit (Colorimetric)
MethylFlash Urine N6-methyladenosine (m6A) Quantification Kit (Colorimetric)

Input Type: Urine
Research Area: RNA Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
P-9015-4848 assays $308.00 Add to Cart
P-9015-9696 assays $509.00 Add to Cart
Research Kits
Result Pages:  1  Displaying 1 to 8 (of 8 products)
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