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RNA Methylation

RNA methylation, such as N6-methyladenosine (m6A), is a key epigenetic modification where methyl groups are added to RNA nucleotides by RNA methyltransferases, like METTL3/14, influencing RNA stability, splicing, translation, and cellular function. This modification is reversible, as RNA demethylases, including FTO and ALKBH5, can remove methyl groups, allowing for dynamic regulation of gene expression.

Chemical diagram illustrating N6-methylation of adenosine in RNA by METTL3 (using SAM as a methyl donor) and demethylation by FTO or ALKBH5 (requiring Fe(II) and 2OG).

Researchers study RNA methylation to understand its roles in development and diseases, as well as to explore its potential for novel therapeutic and diagnostic applications.

Global RNA Modification Quantification Assays

Bar graph showing percentage of 5-methylcytosine (%5-mC) in various DNA sample inputs using the MethylFlash Global DNA Methylation ELISA Kit.

Quantify levels of m6A, 5-mC, or 8-OhG in total RNA

Enzymatic Assays

Agarose gel image comparing bisulfite-converted and unconverted DNA samples using the Methylamp DNA Modification Kit

Measure activity and inhibition of RNA methylases and demethylases

Transcriptome-Specific RNA Methylation

Sanger sequencing chromatograms

Bisulfite and CUT&RUN-based MeRIP assays for methylated RNA analysis

Featured RNA Methylation Kits


	m6A RNA Methylation Quantification Kit Cat #P-9005	CUT&RUN m6A RNA Enrichment (MeRIP) Kit Cat #P-9018	5-mC RNA Methylation ELISA Easy Kit Cat #P-9009
Purpose	An ELISA-like format for absorbance-based quantitation of N6-methyladenosine in RNA	RNA immunoprecipitation (MeRIP) based on CUT&RUN for increased specificity and signals	An ELISA-like format for fluorescence-based quantitation of 5-mC RNA methylation
Key Points	Detect m6A RNA methylation in any species Colorimetric ELISA-like assay using an m6A antibody Detection limit as low as 10 pg of m6A	High Enrichment of m6A Low Sample Requirement Minimal MeRIP/sequencing background	Large signal window, low background noise Detect 5-mC RNA methylation in any species High specificity to 5-mC, with no cross-reactivity
Starting Material	Total RNA	Total RNA	Nuclear Extracts, Purified Enzymes
Lowest Input Amount	100 ng	1 µg - 20 µg	50 ng to 300 ng
Total Protocol Time	< 4 hours	< 3 hours	< 3 hours
Reactions	48,96	24	8,96
Vessel Format	96-Well Plate	Columns/Tubes	96-Well Plate

Global RNA Methylation

Our ELISA-based assays are a simple and convenient way to rapidly screen global RNA modifications, prior to more in-depth and costly approaches like qPCR and NGS. Choose y...

Transcriptome-Specific RNA Methylation

Established methods used for epitranscriptome-wide m6A mapping like MeRIP-seq, PA-m6A-seq, and miCLIP have either been widely used but are unable to achieve high resolution in m6A profiling, or improve the profiling resolution but suffer from poor reproducibility and a complicated process. In parti...

RNA Methylation Activity/Inhibition

The formation of m6A RNA appears to be a co-transcriptional event taking place early on in the RNA lifecycle and is mediated by a multi-protein methyltransferase complex composed, in part, of METTL enzymes. Chemical addition of a methyl group at the nitrogen-6 position of adenosine (A) residues to form m6A occurs via the MET...

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Methylamp RNA Bisulfite Conversion Kit

Methylamp RNA Bisulfite Conversion Kit

Citations (7)

Input Type:	RNA
Research Area:	RNA Methylation
Target Application:	Sample Modification
Vessel Format:	Columns/Tubes

P-9003-050

50 reactions

$199.00

Research Kits

EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

Citations (741) | (12)

Input Type:	RNA
Research Area:	RNA Methylation
Target Application:	Amount Quantitation
Vessel Format:	96-Well Plate

P-9005-48	48 reactions	$352.00
P-9005-96	96 reactions	$581.00

Research Kits

EpiNext 5-mC RNA Bisulfite-Seq Easy Kit (Illumina)

EpiNext 5-mC RNA Bisulfite-Seq Easy Kit (Illumina)

Citations (2)

Input Type:	RNA
Research Area:	Next Gen Sequencing, RNA Methylation
Target Application:	Library Construction, Sample Modification
Vessel Format:	96-Well Plate

P-9007-12	12 reactions	$599.00
P-9007-24	24 reactions	$1,173.00

Research Kits

EpiQuik m6A RNA Methylation Quantification Kit (Fluorometric)

EpiQuik m6A RNA Methylation Quantification Kit (Fluorometric)

Citations (15) | (1)

Input Type:	RNA
Research Area:	RNA Methylation
Target Application:	Amount Quantitation
Vessel Format:	96-Well Plate

P-9008-48	48 assays	$352.00
P-9008-96	96 assays	$581.00

Research Kits

MethylFlash 5-mC RNA Methylation ELISA Easy Kit (Fluorometric)

MethylFlash 5-mC RNA Methylation ELISA Easy Kit (Fluorometric)

Citations (16)

Input Type:	RNA
Research Area:	RNA Methylation
Target Application:	Amount Quantitation
Vessel Format:	96-Well Plate

P-9009-48	48 reactions	$359.00
P-9009-96	96 reactions	$593.00

Research Kits

Epigenase m6A Demethylase Activity/Inhibition Assay Kit (Colorimetric)

Epigenase m6A Demethylase Activity/Inhibition Assay Kit (Colorimetric)

Citations (8)

Input Type:	Nuclear Extracts, Purified Enzymes
Research Area:	RNA Methylation
Target Application:	Amount Quantitation
Vessel Format:	96-Well Plate

P-9013-48	48 assays	$399.00
P-9013-96	96 assays	$646.00

Research Kits

MethylFlash Urine N6-methyladenosine (m6A) Quantification Kit (Colorimetric)

MethylFlash Urine N6-methyladenosine (m6A) Quantification Kit (Colorimetric)

Input Type:	Urine
Research Area:	RNA Methylation
Target Application:	Amount Quantitation
Vessel Format:	96-Well Plate

P-9015-48	48 assays	$364.00
P-9015-96	96 assays	$599.00

Research Kits

EpiNext CUT&RUN RNA m6A-Seq Kit

EpiNext CUT&RUN RNA m6A-Seq Kit

Citations (4) | (1)

Input Type:	RNA
Research Area:	Next Gen Sequencing, RNA Methylation
Target Application:	Library Construction, Sample Modification
Vessel Format:	Columns/Tubes

P-9016-12	12 reactions	$660.00
P-9016-24	24 reactions	$1,158.00

Research Kits

EpiQuik CUT&RUN m6A RNA Enrichment (MeRIP) Kit

EpiQuik CUT&RUN m6A RNA Enrichment (MeRIP) Kit

Citations (106)

Input Type:	RNA
Research Area:	Next Gen Sequencing, RNA Methylation
Target Application:	Library Construction, Sample Modification
Vessel Format:	Columns/Tubes

P-9018-24

24 reactions

$453.00

Research Kits

Epigenase m6A Methylase Activity/Inhibition Assay Kit (Colorimetric)

Epigenase m6A Methylase Activity/Inhibition Assay Kit (Colorimetric)

Citations (7)

Input Type:	Nuclear Extracts, Purified Enzymes
Research Area:	RNA Methylation
Target Application:	Amount Quantitation
Vessel Format:	96-Well Plate

P-9019-48	48 assays	$398.00
P-9019-96	96 assays	$633.00

Research Kits

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Displaying 1 to 10 (of 10 products)

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