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   Home  »  Research Kits  »  RNA Methylation 
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RNA Methylation

RNA Methylation Webinar

What Is RNA Methylation?

RNA methylation is a reversible post-translational modification to RNA that epigenetically impacts numerous biological processes and regulates gene expression. It occurs in different RNAs, including tRNA, rRNA, mRNA, tmRNA, snRNA, snoRNA, miRNA, and viral RNA. Different catalytic strategies are employed for RNA methylation by a variety of RNA-methyltransferases.

What Are Some Specific RNA Modifications?

N6-methyladenosine (m6A) is the most common and abundant methylation modification in RNA molecules present in eukaryotes and accounts for more than 80% of all RNA methylation. It may be referred to as the “fifth RNA base” and has broad roles in regulating embryonic development and cell fates. In particular, m6A methylation is thought to be important for messenger RNA (mRNA) regulation due to enrichment of m6A at 3’UTRs. It has also been linked to cancers, including leukemia, breast cancer, and prostate cancer.

m6AN6-methyladenosine (m6A)

5-methylcytosine (5-mC) in RNA is a post-transcriptional epigenetic mark that is also found to be stable and common in various RNA molecules. Researchers are able to perform RNA bisulfite conversion and RT-PCR amplification, as well as cloning, and sequencing to gain information about RNA cytosine methylation states. By treating RNA with bisulfite, cytosine residues are deaminated to uracil while leaving 5-methylcytosine intact.

Recent data strongly suggest that m6A and 5-mC RNA methylation affects the regulation of various biological processes such as RNA stability and mRNA translation, and that abnormal RNA methylation contributes to etiology of human diseases. Furthermore, loss of 5-mC in vault RNAs causes aberrant processing into Argonaute-associated small RNA fragments that can function as microRNAs. Researchers continue to investigate m6A and 5-mC in RNA to uncover the mysteries of RNA methylation.

To assist you with your studies, EpigenTek offers a complete line of convenient kits to study both RNA and 5-mC methylation. Our product line includes ELISA kits to quickly measure global levels of m6A or 5mC in RNA, meRIP kits to enrich and capture m6A RNA, and ELISA-based assays to rapidly measure m6A methylase activity and m6A demethylase activity.

For additional reading on RNA methylation, check out:

  • Benchtop Tip/Guide for RNA Methylation
  • How CUT&RUN Helps Improve Methylated RNA Immunoprecipitation
  • The Role of m6A RNA Modification in Cancer
  • End-to-End Workflow for Viral and Host RNA Modification Analysis
  • A Review on the Emerging Field of m6A RNA Methylation

Featured RNA Methylation Kits

  m6A RNA Methylation Quantification Kit CUT&RUN m6A RNA Enrichment (MeRIP) Kit 5-mC RNA Methylation ELISA Easy Kit
 

m6A RNA Methylation Quantification Kit Cat #P-9005

CUT&RUN m6A RNA Enrichment (MeRIP) Kit Cat #P-9018

5-mC RNA Methylation ELISA Easy Kit Cat #P-9009

Purpose An ELISA-like format for absorbance-based quantitation of N6-methyladenosine in RNA RNA immunoprecipitation (MeRIP) based on CUT&RUN for increased specificity and signals An ELISA-like format for fluorescence-based quantitation of 5-mC RNA methylation
Key Points
  • Detect m6A RNA methylation in any species
  • Colorimetric ELISA-like assay using an m6A antibody
  • Detection limit as low as 10 pg of m6A
  • High Enrichment of m6A
  • Low Sample Requirement
  • Minimal MeRIP/sequencing background
  • Large signal window, low background noise
  • Detect 5-mC RNA methylation in any species
  • High specificity to 5-mC, with no cross-reactivity
Starting Material Total RNA Total RNA Nuclear Extracts, Purified Enzymes
Lowest Input Amount 50 pg 50-300 ng 2 µg to 20 µg
Total Protocol Time < 4 hours < 3 hours < 3 hours
Reactions 48,96 24 8,96
Vessel Format 96-Well Plate Columns/Tubes 96-Well Plate
Global RNA Methylation
RNA methylation ELISAs are a simple and convenient way to rapidly assess global levels of a specific methylated RNA modification, before pursuing more in-depth and costly applications such as qPCR and ...

Gene-Specific RNA Methylation
Established methods used for epitranscriptome-wide m6A mapping like MeRIP-seq, PA-m6A-seq, and miCLIP have either been widely used but are unable to achieve high resolution in m6A profiling, or improve the profiling resolution but suffer from poor reproducibility and a complicated process. In parti...

RNA Methylation Activity/Inhibition
The formation of m6A RNA appears to be a co-transcriptional event taking place early on in the RNA lifecycle and is mediated by a multi-protein methyltransferase complex composed, in part, of METTL enzymes. Chemical addition of a methyl group at the nitrogen-6 position of adenosine (A) residues to form m6A occurs via the MET...

Result Pages:  1  Displaying 1 to 10 (of 10 products)
Image Description Cat. No. Size Price
Methylamp RNA Bisulfite Conversion Kit
Methylamp RNA Bisulfite Conversion Kit

Citations (4)
Input Type: RNA
Research Area: RNA Methylation
Target Application: Sample Modification
Vessel Format: Columns/Tubes
P-9003-05050 reactions $194.00 Add to Cart
Research Kits
EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)
EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric)

Citations (338) | (10)
Input Type: RNA
Research Area: RNA Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
P-9005-4848 reactions $352.00 Add to Cart
P-9005-9696 reactions $581.00 Add to Cart
Research Kits
EpiNext 5-mC RNA Bisulfite-Seq Easy Kit (Illumina)
EpiNext 5-mC RNA Bisulfite-Seq Easy Kit (Illumina)

Citations (1)
Input Type: RNA
Research Area: Next Gen Sequencing, RNA Methylation
Target Application: Library Construction, Sample Modification
Vessel Format: 96-Well Plate
P-9007-1212 reactions $569.00 Add to Cart
P-9007-2424 reactions $1,114.00 Add to Cart
Research Kits
EpiQuik m6A RNA Methylation Quantification Kit (Fluorometric)
EpiQuik m6A RNA Methylation Quantification Kit (Fluorometric)

Citations (6)
Input Type: RNA
Research Area: RNA Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
P-9008-4848 assays $352.00 Add to Cart
P-9008-9696 assays $581.00 Add to Cart
Research Kits
MethylFlash 5-mC RNA Methylation ELISA Easy Kit (Fluorometric)
MethylFlash 5-mC RNA Methylation ELISA Easy Kit (Fluorometric)

Citations (5)
Input Type: RNA
Research Area: RNA Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
P-9009-4848 reactions $352.00 Add to Cart
P-9009-9696 reactions $581.00 Add to Cart
Research Kits
Epigenase m6A Demethylase Activity/Inhibition Assay Kit (Colorimetric)
Epigenase m6A Demethylase Activity/Inhibition Assay Kit (Colorimetric)

Citations (3)
Input Type: Nuclear Extracts, Purified Enzymes
Research Area: RNA Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
P-9013-4848 assays $386.00 Add to Cart
P-9013-9696 assays $613.00 Add to Cart
Research Kits
MethylFlash Urine N6-methyladenosine (m6A) Quantification Kit (Colorimetric)
MethylFlash Urine N6-methyladenosine (m6A) Quantification Kit (Colorimetric)

Input Type: Urine
Research Area: RNA Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
P-9015-4848 assays $346.00 Add to Cart
P-9015-9696 assays $569.00 Add to Cart
Research Kits
EpiNext CUT&RUN RNA m6A-Seq Kit
EpiNext CUT&RUN RNA m6A-Seq Kit

Citations (1)
Input Type: RNA
Research Area: Next Gen Sequencing, RNA Methylation
Target Application: Library Construction, Sample Modification
Vessel Format: Columns/Tubes
P-9016-1212 reactions $635.00 Add to Cart
P-9016-2424 reactions $1,113.00 Add to Cart
Research Kits
EpiQuik CUT&RUN m6A RNA Enrichment (MeRIP) Kit
EpiQuik CUT&RUN m6A RNA Enrichment (MeRIP) Kit

Citations (19)
Input Type: RNA
Research Area: Next Gen Sequencing, RNA Methylation
Target Application: Library Construction, Sample Modification
Vessel Format: Columns/Tubes
P-9018-2424 reactions $436.00 Add to Cart
Research Kits
Epigenase m6A Methylase Activity/Inhibition Assay Kit (Colorimetric)
Epigenase m6A Methylase Activity/Inhibition Assay Kit (Colorimetric)

Citations (2)
Input Type: Nuclear Extracts, Purified Enzymes
Research Area: RNA Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
P-9019-4848 assays $378.00 Add to Cart
P-9019-9696 assays $602.00 Add to Cart
Research Kits
Result Pages:  1  Displaying 1 to 10 (of 10 products)
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