Research Kits

.expiring-banner { display: none; } // Set the expiration date (March 31, 2025) const expirationDate = new Date('2025-04-30T23:59:59'); // Get the current date const currentDate = new Date(); // When DOM is ready, check the expiration and show banners if needed document.addEventListener("DOMContentLoaded", function() { const banners = document.querySelectorAll('.expiring-banner'); if (currentDate { banner.style.display = 'block'; }); } }); Use code E2504EXD to take 10% Off All Sample Preparation Kits through April* Rapid and efficient preparation of DNA, proteins, and chromatin is vital in performing downstream analyses. Our suite of sample preparation kits are suitable for nearly any workflow, whether it be isolating chromatin for ChIP-Seq or ChIP-PCR applications or preparing DNA from a variety of starting materials for Bisulfite-Seq or MS-PCR. Our sample preparation kits enable you to go directly from extraction to reaction, offering countless possibilities and flexibility in your experimental workflow. Our DNA, protein, and chromatin preparation kits are the starting point for endless research opportunities and downstream applications.

Next generation sequencing (NGS) is a method of non-Sanger-based high throughput DNA sequencing in which millions or even billions of DNA strands can be sequenced in parallel. Epigenetic researchers utilize NGS as an important tool to gain detailed and comprehensive insight into epigenetic modifications of genomes belonging to numerous species and from many different cell types. NGS minimizes the need for fragment-cloning methods often used in Sanger sequencing of genomes and yields significantly more throughput.

DNA methylation is an epigenetic modification where a methyl group is added to cytosine bases, typically at CpG sites, by DNA methyltransferases (DNMTs), influencing gene expression without altering the DNA sequence. This modification can be dynamically regulated, as ten-eleven translocation (TET) enzymes catalyze the oxidation of 5-methylcytosine (5-mC...

RNA methylation, such as N6-methyladenosine (m6A), is a key epigenetic modification where methyl groups are added to RNA nucleotides by RNA methyltransferases, like METTL3/14, influencing RNA stability, splicing, translation, and cellular function. This modification is reversible, as RNA demethylases, including FTO and ALKBH5...

Chromatin studies, including chromatin immunoprecipitation (ChIP) and chromatin accessibility assays, are essential for understanding how DNA is packaged and regulated within the nucleus. These techniques help identify protein-DNA interactions, protein post-translational modifications, and accessible regulatory regions that influence gene expr...

Histone Modifications Histone modifications, such as methylation, acetylation, phosphorylation, citrullination, and sumoylation, are epigenetic marks that regulate chromatin structure and gene expres...

RNA Immunoprecipitation (RIP) is a widely used method for studying interactions between RNA molecules and RNA-binding proteins (RBPs). This technique enables researchers to investigate post-transcriptional regulation, RNA stability, splicing, localization, and translation, all of which are mediated by RBPs. RIP involves the immunoprecipitation of RNA-protein complexes from cell or tissue lysates using antibodies specific to the RBP of interest. The captured RNA can then be analyzed using qRT-PCR, microarrays, or next-generation sequencing (RIP-Seq) to identify and quantify the RNA species bound to the target protein. Over the years, RIP has become an indispensable tool for understanding the epitranscriptome and the dynamics of protein-RNA interactions.

Histone acetylation and histone deacetylation involve the addition or removal of an acetyl group on lysine residues in the N-terminal tail and on the surface of the nucelosome core of histone proteins. Acetylated and deacetylated histones are considered epigenetic tags within chromatin by relaxing (euchromatin) or tightening (heterochromatin) chromatin structure, subsequently increasing or decreasing gene transcription levels.

DNA and RNA damage refers to the structural and functional alteration of these nucleic acids. It can be caused by several factors, including oxidative stress, genotoxic/cytotoxic agents, ionizing radiation, industrial chemicals, and cancer chemotherapy. The Role of Oxidative Stress in DNA Damage Oxidative stress has been identified as one of the greatest factors in DNA and RNA damage. It occurs when there is an imbalance between the production of reactive oxygen s...

Targeted gene knockdown using small interfering RNA (siRNA) or antisense oligonucleotides has been valuable technology in studying gene function. Gene knockdown leads to the reduction of messenger RNA and subsequently decreased protein expression. Alternatively, CRISPR/Cas9-based gene editing is also a versatile method to alter the genome. These gene editing and silencing methods give researchers valuable insight into biological processes and especially diseases.

Various histone modifications, such as histone citrullination and histone phosphorylation, have been shown to epigenetically impact gene expression through different mechanisms. Adjusting epigenetic marks and changing chromatin structure – known as chromatin remodeling – as a result of these histone modifications can influence gene regulation in many tissue types. Studying these histone modifications provides researchers with valuable insight into cellular processes such as apoptosis, cel...