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EpiQuik Total Histone Extraction Kit

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For isolating histone proteins with post-translational modifications (PTMs) intact

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Schematic procedure of chromation isolation with the EpiQuik™ Total Histone Extraction Kit.
Histone extracts were prepared from MCF-7 cells using the EpiQuik Total Histone Extraction Kit and acetyl histone H3-K9 was quantified using the EpiQuik Global Acetylated Histone H3K9 Quantification Kit (Colorimetric) (Cat. No. P-4010).
SDS-PAGE analysis of histone extracts was prepared with the EpiQuik™ Total Histone Extraction Kit. 10 µg of each sample were loaded per lane (1-8).
Input Type: Unisolated Samples
Research Area: Histone Acetylation, Histone Methylation
Target Application: Sample Isolation
Vessel Format: Columns/Tubes
100% Guarantee: 6 months
Catalog No.SizePriceQty
OP-0006-100100 extractions $114.00 
Availability: Usually Ships in 1 Day or Same day delivery Same Day NY Delivery 
Product Overview

The EpiQuik™ Total Histone Extraction Kit is a complete set of optimized buffers and reagents for extracting total core histone proteins (H2A, H2B, H3, and H4) from mammalian cells or tissues in a simple 60 minute procedure. The post-translational modifications (PTM) in the histone extracts are kept intact and thus can be used with Epigentek's histone modification assay kits  or in a variety of downstream applications for histone methylation, acetylation, phosphorylation, sumoylation, ubiquitination, citrullination, and ADP-ribosylation  studies.

  • Pre-optimized and simple 1 hour protocol.
  • Conveniently includes all essential reagents to carry out a histone extraction.
  • Standardized procedure for reproducible results.
  • Extracts a high yield of total core histones from as little as 1 mg of tissues.
  • Post-translational modifications are kept intact.
  • Does not affect histone modification status or levels.

Background Information
Histones are the chief protein components of chromatin in biology. They act as spools around which DNA winds, and also play a role in gene regulation. The core histones include H2A, H2B, H3, and H4. Histones undergo post-translational modifications, which alter their interaction with DNA and nuclear proteins. The H3 and H4 histones have long tails protruding from the nucleosome, which can be covalently modified at several places (i.e., methylation, acetylation, phosphorylation, etc.). Histone modifications act in diverse biological processes such as gene regulation, DNA repair and chromosome condensation (mitosis). Combinations of modifications are thought to constitute a "histone code" and an understanding of this code may help to answer questions about human development and disease.

Principle & Procedure
The EpiQuik™ Total Histone Extraction Kit simply applies our proprietary histone isolation buffers to cell or tissue samples. After treatment with pre-lysis, lysis, and balance buffers, the total core histones are easily extracted for immediate use or storage under proper conditions.

Starting Materials
The minimal amount of starting materials can be as low as 10cells or 1 mg tissue. For the best results, the cell number should be greater than 106 cells or the tissue amount should be greater than 10 mg. A total of 100 standard extractions (using 107 cells or 100 mg tissues per extraction) can be performed with this kit. Yield of the total histone proteins is approximately 0.4 mg per 107 cells or 100 mg of tissue. The yield may vary depending on cell or tissue type.

Product Components

10X Pre-Lysis Buffer
Lysis Buffer
Balance Buffer
DTT Solution
User Guide

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

Product Citations

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Customer Reviews

Rating By f*********@bcm.edu Verified Purchase Reviewed on: Monday 17 July, 2017
Application Description
We used the kit to extract the total histone from cells. Then westenblot was performed to investigate the biological activity of compounds in epigenetic level. Till now, this kit performs really good. You can control the final histone concentration via the volume of reagent used.

Pros:
It is easy to use. The most important is it works.

Cons:
There is no box (only one plastic bag) to include all the reagents. It is very easy to miss some reagents

Other Thoughts
How friendly was the user guide? (1 – worst, 5 – best)
5

How professional was the appearance and presentation of the product? (1 – worst, 5 – best)
3



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