Save $100 on Antibodies Save $100 when you buy $500 in antibodies. Use code E1705ABR. Expires June 30th. » Learn More
Epigentek Home
Epigentek Free Shipping

EpiQuik Total Histone Extraction Kit


For isolating histone proteins with post-translational modifications (PTMs) intact

Citations (103) | Write a Review
Schematic procedure of chromation isolation with the EpiQuik™ Total Histone Extraction Kit.
Histone extracts were prepared from MCF-7 cells using the EpiQuik Total Histone Extraction Kit and acetyl histone H3-K9 was quantified using the EpiQuik Global Acetylated Histone H3K9 Quantification Kit (Colorimetric) (Cat. No. P-4010).
SDS-PAGE analysis of histone extracts was prepared with the EpiQuik™ Total Histone Extraction Kit. 10 µg of each sample were loaded per lane (1-8).
Input Type: Unisolated Samples
Research Area: Histone Acetylation, Histone Methylation
Target Application: Sample Isolation
Vessel Format: Columns/Tubes
100% Guarantee: 6 months
Catalog No.SizePriceQty
OP-0006-100100 extractions $114.00 
Availability: Usually Ships in 1 Day or Same day delivery Same Day NY Delivery 
Product Overview

The EpiQuik™ Total Histone Extraction Kit is a complete set of optimized buffers and reagents for extracting total core histone proteins (H2A, H2B, H3, and H4) from mammalian cells or tissues in a simple 60 minute procedure. The post-translational modifications (PTM) in the histone extracts are kept intact and thus can be used with Epigentek's histone modification assay kits  or in a variety of downstream applications for histone methylation, acetylation, phosphorylation, sumoylation, ubiquitination, citrullination, and ADP-ribosylation  studies.

  • Pre-optimized and simple 1 hour protocol.
  • Conveniently includes all essential reagents to carry out a histone extraction.
  • Standardized procedure for reproducible results.
  • Extracts a high yield of total core histones from as little as 1 mg of tissues.
  • Post-translational modifications are kept intact.
  • Does not affect histone modification status or levels.

Background Information
Histones are the chief protein components of chromatin in biology. They act as spools around which DNA winds, and also play a role in gene regulation. The core histones include H2A, H2B, H3, and H4. Histones undergo post-translational modifications, which alter their interaction with DNA and nuclear proteins. The H3 and H4 histones have long tails protruding from the nucleosome, which can be covalently modified at several places (i.e., methylation, acetylation, phosphorylation, etc.). Histone modifications act in diverse biological processes such as gene regulation, DNA repair and chromosome condensation (mitosis). Combinations of modifications are thought to constitute a "histone code" and an understanding of this code may help to answer questions about human development and disease.

Principle & Procedure
The EpiQuik™ Total Histone Extraction Kit simply applies our proprietary histone isolation buffers to cell or tissue samples. After treatment with pre-lysis, lysis, and balance buffers, the total core histones are easily extracted for immediate use or storage under proper conditions.

Starting Materials
The minimal amount of starting materials can be as low as 10cells or 1 mg tissue. For the best results, the cell number should be greater than 106 cells or the tissue amount should be greater than 10 mg. A total of 100 standard extractions (using 107 cells or 100 mg tissues per extraction) can be performed with this kit. Yield of the total histone proteins is approximately 0.4 mg per 107 cells or 100 mg of tissue. The yield may vary depending on cell or tissue type.

Product Components

10X Pre-Lysis Buffer
Lysis Buffer
Balance Buffer
DTT Solution
User Guide

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing along with your contact information and institution name.

Product Citations

Zhou Z et. al. (May 2017). Loss of TET1 facilitates DLD1 colon cancer cell migration via H3K27me3-mediated down-regulation of E-cadherin. J Cell Physiol.

Clancy KW et. al. (May 2017). Citrullination/Methylation Crosstalk on Histone H3 Regulates ER-Target Gene Transcription. ACS Chem Biol.

Ding R et. al. (March 2017). Dose- and time- effect responses of DNA methylation and histone H3K9 acetylation changes induced by traffic-related air pollution. Sci Rep. 7:43737.

Kayashima Y et. al. (March 2017). Alkylresorcinols activate SIRT1 and delay ageing in Drosophila melanogaster. Sci Rep. 7:43679.

Cong G et. al. (February 2017). Involvement of histone methylation in macrophage apoptosis and unstable plaque formation in methionine-induced hyperhomocysteinemic ApoE<sup>-/-</sup> mice. Life Sci.

Wang R et. al. (January 2017). Histone H4 expression is cooperatively maintained by IKKβ and Akt1 which attenuates cisplatin-induced apoptosis through the DNA-PK/RIP1/IAPs signaling cascade. Sci Rep. 7:41715.

Torres-Pérez JV et. al. (January 2017). Phosphorylated Histone 3 at Serine 10 Identifies Activated Spinal Neurons and Contributes to the Development of Tissue Injury-Associated Pain. Sci Rep. 7:41221.

Adamu HA et. al. (January 2017). In utero exposure to germinated brown rice and its oryzanol-rich extract attenuated high fat diet-induced insulin resistance in F1 generation of rats. BMC Complement Altern Med. 17(1):67.

Min C et. al. (January 2017). Selective Inhibitors of Histone Deacetylases 1 and 2 Synergize with Azacitidine in Acute Myeloid Leukemia. PLoS One. 12(1):e0169128.

Benoy V et. al. (December 2016). Development of Improved HDAC6 Inhibitors as Pharmacological Therapy for Axonal Charcot-Marie-Tooth Disease. Neurotherapeutics.

Yang C et. al. (November 2016). CD47 is a Potential Target for the Treatment of Laryngeal Squamous Cell Carcinoma. Cell Physiol Biochem. 40(1-2):126-136.

Francesca Mattioli et. al. (November 2016). Mutations in Histone Acetylase Modifier BRPF1 Cause an Autosomal-Dominant Form of Intellectual Disability with Associated Ptosis AJHG.

Kanwal R et. al. (September 2016). Dietary Flavones as Dual Inhibitors of DNA Methyltransferases and Histone Methyltransferases. PLoS One. 11(9):e0162956.

Xiong Guo et. al. (September 2016). miR-15b facilitates the progression of colorectal cancer via targeting β-catenin and Axin2 IJCEP. 9(9):8990-8996.

Dincman TA et. al. (August 2016). Histone deacetylase inhibition is cytotoxic to oligodendrocyte precursor cells in vitro and in vivo. Int J Dev Neurosci. 54:53-61.

Qi W et. al. (August 2016). Abnormal histone acetylation of CD8+ T cells in patients with severe aplastic anemia. Int J Hematol.

Xu S et. al. (July 2016). Tip60 HAT Action Mediates Environmental Enrichment Induced Cognitive Restoration. PLoS One. 11(7):e0159623.

Lee HO et. al. (July 2016). Lack of global epigenetic methylation defects in CBS deficient mice. J Inherit Metab Dis.

Guo S et. al. (June 2016). Characteristics and expression patterns of histone-modifying enzyme systems in the migratory locust. Insect Biochem Mol Biol. 76:18-28.

Zhao Y et. al. (June 2016). Dynamic Variation of Histone H3 Trimethyl Lys4 (H3K4me3) and Heterochromatin Protein 1 (HP1) with Employment Length in Nickel Smelting Workers. Biomarkers. :1-20.

Kallingappa PK et. al. (June 2016). Quiescence Loosens Epigenetic Constraints in Bovine Somatic Cells and Improves Their Reprogramming into Totipotency. Biol Reprod.

Hu C et. al. (June 2016). Frontline Science: ATF3 is responsible for the inhibition of TNF-α release and the impaired migration of acute ethanol-exposed monocytes and macrophages. J Leukoc Biol.

Vinogradova M et. al. (May 2016). An inhibitor of KDM5 demethylases reduces survival of drug-tolerant cancer cells. Nat Chem Biol.

Iwanowycz S et. al. (May 2016). Emodin inhibits breast cancer growth by blocking the tumor-promoting feedforward loop between cancer cells and macrophages. Mol Cancer Ther.

Li N et. al. (May 2016). Mutations in the Histone Modifier PRDM6 Are Associated with Isolated Nonsyndromic Patent Ductus Arteriosus. Am J Hum Genet.

Ruiz-Andres O et. al. (April 2016). Histone lysine-crotonylation in acute kidney injury. Dis Model Mech.

Gang Yu et. al. (April 2016). Ozone therapy could attenuate tubulointerstitial injury in adenine-induced CKD rats by mediating Nrf2 and NF-κB IJBMS. 19(10)

Shearstone JR et. al. (April 2016). Chemical Inhibition of Histone Deacetylases 1 and 2 Induces Fetal Hemoglobin through Activation of GATA2. PLoS One. 11(4):e0153767.

Muta K et. al. (March 2016). Curcumin ameliorates nephrosclerosis via suppression of histone acetylation independent of hypertension Nephrol Dial Transplant.

Snigdha S et. al. (March 2016). H3K9me3 Inhibition Improves Memory, Promotes Spine Formation, and Increases BDNF Levels in the Aged Hippocampus. J Neurosci.

Eid A et. al. (February 2016). Developmental lead exposure and lifespan alterations in epigenetic regulators and their correspondence to biomarkers of Alzheimer's disease Alzheimer's and Dement. :1-9.

Liu Y et. al. (February 2016). Effects of Wutou Decoction on DNA Methylation and Histone Modifications in Rats with Collagen-Induced Arthritis Evid. Based Complement. Alternat. Med.. 2016:1-9.

Adamu HA et. al. (February 2016). Perinatal exposure to germinated brown rice and its gamma amino-butyric acid-rich extract prevents high fat diet-induced insulin resistance in first generation rat offspring. Food Nutr Res. 60:30209.

Smonskey M et. al. (January 2016). EZH2 inhibition re-sensitizes multidrug resistant B-cell lymphomas to etoposide mediated apoptosis Oncoscience. 3(1):1-10.

Zheng YC et. al. (January 2016). 1,2,3-Triazole-Dithiocarbamate Hybrids, a Group of Novel Cell Active SIRT1 Inhibitors. Cell Physiol Biochem. 38(1):185-93.

Sant KE et. al. (January 2016). Mono-2-ethylhexyl phthalate (MEHP) alters histiotrophic nutrition pathways and epigenetic processes in the developing conceptus. J Nutr Biochem. 27:211-8.

Burchfield JS et. al. (January 2016). Pharmacological priming of adipose-derived stem cells promotes myocardial repair. J Investig Med. 64(1):50-62.

Wu F et. al. (December 2015). 3-(Piperidin-4-ylmethoxy)pyridine Containing Compounds Are Potent Inhibitors of Lysine Specific Demethylase 1. J Med Chem.

Zeng Q et. al. (December 2015). Apoptosis in human myelodysplastic syndrome CD34+ cells is modulated by the upregulation of TLRs and histone H4 acetylation via a β-arrestin 1 dependent mechanism Exp Cell Res.

Inagaki Y et. al. (December 2015). Epigenetic regulation of proliferation and invasion in hepatocellular carcinoma cells by CBP/p300 histone acetyltransferase activity. Int J Oncol.

Huynh et. al. (November 2015). Inhibition of Histone Deacetylases Enhances the Osteogenic Differentiation of Human Periodontal Ligament Cells J Cell Biol.

Han Y et. al. (November 2015). Novel Tranylcypromine/Hydroxylcinnamic Acid Hybrids as Lysine-Specific Demethylase 1 Inhibitors with Potent Antitumor Activity. Chem Pharm Bull (Tokyo). 63(11):882-9.

Ma L et. al. (October 2015). Histone Methylation in Nickel-Smelting Industrial Workers. PLoS One. 10(10):e0140339.

Sancak D et. al. (October 2015). Global histone modifications in Fumonisin B1 exposure in rat kidney epithelial cells. Toxicol In Vitro. 29(7):1809-15.

Keenan MM et. al. (October 2015). ACLY and ACC1 Regulate Hypoxia-Induced Apoptosis by Modulating ETV4 via α-ketoglutarate. PLoS Genet. 11(10):e1005599.

Li MF et. al. (September 2015). High Glucose Increases the Expression of Inflammatory Cytokine Genes in Macrophages Through H3K9 Methyltransferase Mechanism. J Interferon Cytokine Res.

Zhuo X et. al. (August 2015). Usp16 regulates kinetochore localization of Plk1 to promote proper chromosome alignment in mitosis. J Cell Biol. 210(5):727-35.

Finegersh A et. al. (August 2015). Repeated vapor ethanol exposure induces transient histone modifications in the brain that are modified by genotype and brain region Front Mol Neurosci. 8:39.

Plagg B et. al. (July 2015). Increased Acetylation of Histone H4 at Lysine 12 (H4K12) in Monocytes of Transgenic Alzheimer's Mice and in Human Patients. Curr Alzheimer Res. 12(8):752-60.

Yan N et. al. (June 2015). Histone hypoacetylation and increased histone deacetylases in peripheral blood mononuclear cells from patients with Graves' disease. Mol Cell Endocrinol.

Kim KP et. al. (June 2015). First-in-human study of the toxicity, pharmacokinetics, and pharmacodynamics of CG200745, a pan-HDAC inhibitor, in patients with refractory solid malignancies. Invest New Drugs.

Puig-Vilanova E et. al. (June 2015). Quadriceps muscle weakness and atrophy are associated with a differential epigenetic profile in advanced COPD. Clin Sci (Lond). 128(12):905-21.

Wang J et. al. (March 2015). Cadmium induced apoptosis in mouse primary hepatocytes: the role of oxidative stress-mediated ERK pathway activation and the involvement of histone H3 phosphorylation RSC Advances. 5:31798-31806.

Tu P et. al. (March 2015). Liver histone H3 methylation and acetylation may associate with type 2 diabetes development. J Physiol Biochem. 71(1):89-98.

Ma LY et. al. (February 2015). Design, Synthesis, and Structure-Activity Relationship of Novel LSD1 Inhibitors Based on Pyrimidine-Thiourea Hybrids As Potent, Orally Active Antitumor Agents. J Med Chem. 58(4):1705-16.

Kirk JS et. al. (February 2015). Top2a identifies and provides epigenetic rationale for novel combination therapeutic strategies for aggressive prostate cancer. Oncotarget. 6(5):3136-46.

Li C et. al. (February 2015). DNA methylation and histone modification patterns during the late embryonic and early postnatal development of chickens. Poult Sci.

Geis-Asteggiante L et. al. (February 2015). Top-down analysis of low mass proteins in exosomes shed by murine myeloid-derived suppressor cells. Int J Mass Spectrom. 378:264-269.

Pacaud R et. al. (January 2015). Histone H3 Phosphorylation in GBM: a New Rational to Guide the Use of Kinase Inhibitors in anti-GBM Therapy. Theranostics. 5(1):12-22.

Rouleau SG et. al. (January 2015). Small antisense oligonucleotides against G-quadruplexes: specific mRNA translational switches. Nucleic Acids Res. 43(1):595-606.

Zhu G et. al. (December 2014). Elevated expression of histone demethylase PHF8 associates with adverse prognosis in patients of laryngeal and hypopharyngeal squamous cell carcinoma. Epigenomics. :1-11.

Burke MC et. al. (December 2014). Ubiquitinated proteins in exosomes secreted by myeloid-derived suppressor cells. J Proteome Res. 13(12):5965-72.

Zhang L et. al. (November 2014). Inhibition of histone H3K79 methylation selectively inhibits proliferation, self-renewal and metastatic potential of breast cancer. Oncotarget. 5(21):10665-77.

Li C et. al. (November 2014). Maternal high-zinc diet attenuates intestinal inflammation by reducing DNA methylation and elevating H3K9 acetylation in the A20 promoter of offspring chicks. J Nutr Biochem.

Dong G et. al. (September 2014). Feeding a high-concentrate corn straw diet induced epigenetic alterations in the mammary tissue of dairy cows. PLoS One. 9(9):e107659.

Schulz MD et. al. (August 2014). High-fat-diet-mediated dysbiosis promotes intestinal carcinogenesis independently of obesity. Nature.

Geis-Asteggiante L et. al. (August 2014). Top–down analysis of low mass proteins in exosomes shed by murine myeloid-derived suppressor cells International Journal of Mass Spectrometry. Abstract

Kumar P et. al. (June 2014). All-trans retinoic acid and sodium butyrate enhance natriuretic peptide receptor a gene transcription: role of histone modification. Mol Pharmacol. 85(6):946-57.

Dong Y et. al. (May 2014). H2AX phosphorylation regulated by p38 is involved in Bim expression and apoptosis in chronic myelogenous leukemia cells induced by imatinib. Apoptosis.

Elhammali A et. al. (April 2014). A High-Throughput Fluorimetric Assay for 2-Hydroxyglutarate Identifies Zaprinast as a Glutaminase Inhibitor. Cancer Discov.

Kraus D et. al. (April 2014). Nicotinamide N-methyltransferase knockdown protects against diet-induced obesity. Nature. 508(7495):258-62.

Siuda D et. al. (April 2014). Social isolation-induced epigenetic changes in midbrain of adult mice. J Physiol Pharmacol. 65(2):247-55.

Tafrova JI et. al. (March 2014). Human histone acetyltransferase 1 (Hat1) acetylates lysine 5 of histone H2A in vivo. Mol Cell Biochem.

Kumar P et. al. (March 2014). Histone deacetylase inhibitors modulate the transcriptional regulation of guanylyl cyclase/natriuretic peptide receptor-a gene: interactive roles of modified histones, histone acetyltransferase, p300, AND Sp1. J Biol Chem. 289(10):6991-7002.

Liu D et. al. (March 2014). Histone acetylation and expression of mono-aminergic transmitters synthetases involved in CUS-induced depressive rats. Exp Biol Med (Maywood). 239(3):330-6.

Monteiro JB et. al. (March 2014). Endometriosis is characterized by a distinct pattern of histone 3 and histone 4 lysine modifications. Reprod Sci. 21(3):305-18.

Jochems J et. al. (January 2014). Antidepressant-like properties of novel HDAC6-selective inhibitors with improved brain bioavailability. Neuropsychopharmacology. 39(2):389-400.

Zheng YC et. al. (November 2013). Triazole-dithiocarbamate based selective lysine specific demethylase 1 (LSD1) inactivators inhibit gastric cancer cell growth, invasion, and migration. J Med Chem. 56(21):8543-60.

Aguilar-Valles A et. al. (October 2013). Methamphetamine-Associated Memory Is Regulated by a Writer and an Eraser of Permissive Histone Methylation. Biol Psychiatry.

Rossi D et. al. (August 2013). Croton lechleri Müll. Arg. (Euphorbiaceae) stem bark essential oil as possible mutagen-protective food ingredient against heterocyclic amines from cooked food. Food Chem. 139(1-4):439-47.

Navrkalova V et. al. (August 2013). The p53 pathway induction is not primarily dependent on Ataxia Telangiectasia Mutated (ATM) gene activity after fludarabine treatment in chronic lymphocytic leukemia cells. Leuk Lymphoma. 54(8):1840-3.

Shyamasundar S et. al. (June 2013). Analysis of epigenetic factors in mouse embryonic neural stem cells exposed to hyperglycemia. PLoS One. 8(6):e65945.

Itzhak Y et. al. (May 2013). Sodium butyrate-induced histone acetylation strengthens the expression of cocaine-associated contextual memory. Neurobiol Learn Mem. 102:34-42.

Deng L et. al. (May 2013). Synthesis, Activity and Metabolic Stability of Non-Ribose Containing Inhibitors of Histone Methyltransferase DOT1L. Medchemcomm. 4(5):822-826.

Luo S et. al. (March 2013). Aberrant histone modifications in peripheral blood mononuclear cells from patients with Henoch-Schönlein purpura. Clin Immunol. 146(3):165-75.

Meng F et. al. (February 2013). Anticancer efficacy of cisplatin and trichostatin A or 5-aza-2'-deoxycytidine on ovarian cancer. Br J Cancer. 108(3):579-86.

Timofeeva OA et. al. (January 2013). STAT3 suppresses transcription of proapoptotic genes in cancer cells with the involvement of its N-terminal domain. Proc Natl Acad Sci U S A. 110(4):1267-72.

Kao GS et. al. (December 2012). Neonatal isolation decreases cued fear conditioning and frontal cortical histone 3 lysine 9 methylation in adult female rats. Eur J Pharmacol. 697(1-3):65-72.

McCabe MT et. al. (December 2012). EZH2 inhibition as a therapeutic strategy for lymphoma with EZH2-activating mutations. Nature. 492(7427):108-12.

Fan H et. al. (December 2012). Sulforaphane causes a major epigenetic repression of myostatin in porcine satellite cells. Epigenetics. 7(12):1379-90.

Rehan VK et. al. (October 2012). Perinatal nicotine exposure induces asthma in second generation offspring. BMC Med. 10:129.

Yamamura Y et. al. (August 2012). TNF-α inhibits aquaporin 5 expression in human salivary gland acinar cells via suppression of histone H4 acetylation. J Cell Mol Med. 16(8):1766-75.

Qu F et. al. (August 2012). A molecular mechanism underlying ovarian dysfunction of polycystic ovary syndrome: hyperandrogenism induces epigenetic alterations in the granulosa cells. J Mol Med (Berl). 90(8):911-23.

Wurm T et. al. (July 2012). The HTLV-1-encoded protein HBZ directly inhibits the acetyl transferase activity of p300/CBP. Nucleic Acids Res. 40(13):5910-25.

Itzhak Y et. al. (May 2012). Histone acetylation rescues contextual fear conditioning in nNOS KO mice and accelerates extinction of cued fear conditioning in wild type mice. Neurobiol Learn Mem. 97(4):409-17.

Zhao M et. al. (February 2012). Abnormal epigenetic modifications in peripheral blood mononuclear cells from patients with alopecia areata. Br J Dermatol. 166(2):226-73.

Ponomarev I et. al. (February 2012). Gene coexpression networks in human brain identify epigenetic modifications in alcohol dependence. J Neurosci. 32(5):1884-97.

Neri F et. al. (February 2012). Myc regulates the transcription of the PRC2 gene to control the expression of developmental genes in embryonic stem cells. Mol Cell Biol. 32(4):840-51.

Jing L et. al. (April 2011). Effect of the histone deacetylase inhibitors on behavioural sensitization to a single morphine exposure in mice. Neurosci Lett. 494(2):169-73.

Kawakami K et. al. (January 2011). Long interspersed nuclear element-1 hypomethylation is a potential biomarker for the prediction of response to oral fluoropyrimidines in microsatellite stable and CpG island methylator phenotype-negative colorectal cancer. Cancer Sci. 102(1):166-74.

Hansen A et. al. (July 2010). Development of a drug screening platform based on engineered heart tissue. Circ Res. 107(1):35-44.

Diehl F et. al. (March 2010). Cardiac deletion of Smyd2 is dispensable for mouse heart development. PLoS One. 5(3):e9748.

Replace Your Santa Cruz Abs
Related Products

Researchers Also Purchased
EpiQuik Histone H3 Modification Multiplex Assay Kit (Colorimetric)
EpiQuik Histone H3 Modification Multiplex Assay Kit (Colorimetric)
EpiQuik Nuclear Extraction Kit
EpiQuik Nuclear Extraction Kit
EpiQuik Histone H4 Modification Multiplex Assay Kit (Colorimetric)
EpiQuik Histone H4 Modification Multiplex Assay Kit (Colorimetric)
MethylFlash Methylated DNA 5-mC Quantification Kit (Colorimetric)
MethylFlash Methylated DNA 5-mC Quantification Kit (Colorimetric)
Epigentek is an epigenetics company that specializes in epigenetic kits, antibodies, reagents, and services for epigenetic research in DNA methylation, histone modification, and chromatin studies. Terms & Conditions | Privacy Policy | Site Map
Copyright © 2017 Epigentek Group Inc. All rights reserved.