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EpiQuik Nuclear Extraction Kit

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For isolation of nuclear proteins from cells or tissues while keeping enzymatic activity intact

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To demonstrate the quality of the materials and protocol of the EpiQuik Nuclear Extraction Kit, nuclear extracts were prepared from MCF-7 cells and the activity of HDACs were measured using different amounts of the extract. The result shown in the figure demonstrates the kit's high specificity.
Input Type: Unisolated Samples
Research Area: DNA Methylation, Histone Acetylation, Histone Methylation, Sumoylation
Target Application: Sample Isolation
Vessel Format: Columns/Tubes
100% Guarantee: 6 months
Catalog No.SizePriceQty
OP-0002-1100 assays $158.00 
Availability: Usually Ships in 1 Day or Same day delivery Same Day NY Delivery 
Product Overview

The EpiQuik™ Nuclear Extraction Kit is a complete set of optimized reagents to provide a simple and selective method for isolating nuclear proteins used for a variety of applications in just 60 minutes. These applications may include Western blot, protein-DNA binding assays, nuclear enzyme assays, and any other procedures requiring optimized nuclear proteins. The protocol is fast and easy-to-use, and also isolates very abundant yields of nuclear extract from mammalian cells or tissue samples. The kit is also specifically designed to meet the requirements of nuclear extracts used in various other epigenetic products from Epigentek, including DNMTHDAC, and HAT assays. Some of the kit's highlighted features include the following:

  • Pre-optimized and simple step-wise 1 hour protocol.
  • Conveniently includes all essential reagents to carry out a nuclear extraction.
  • Standardized procedure for reproducible results.

Principle & Procedure
The EpiQuik™ Nuclear Extraction Kit simply applies our proprietary nuclear protein isolation buffers to cell/tissues. After treatment with pre-lysis and lysis buffers, the nuclear proteins are easily extracted for immediate use or storage at proper conditions.

Starting Materials & Yield
A total of 100 standard extractions (using 10^7 cells or 20 mg tissues) can be isolated from mammalian cells or tissue samples with this kit. Total yield can be up to 100 µg per optimal extraction, although the results may somewhat vary depending on the cell or tissue type.

Product Components

NE1 (10X Pre-Extraction Buffer)
NE2 (Extraction Buffer)
PIC (1000X Protease Inhibitor Cocktail)
1000X DTT Solution
User Guide

Frequently Asked Q's

1. Should DTT be added into NE1 for tissue extraction?
It is not necessary.

2. How many rpm should be used for centrifuging the cell extract if using a desktop centrifuge?
12,000-14,000 rpm.

3. What should I do if the addition of 5 µl NE2 into 1 M of cells cannot suspend the nuclei?
Add more NE2. For example, add 10 µl to 1 M nuclei.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]
Product Citations

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Maeda R et. al. (March 2012). Immunostimulatory activity of polysaccharides isolated from Caulerpa lentillifera on macrophage cells. Biosci Biotechnol Biochem. 76(3):501-5.

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Neri F et. al. (February 2012). Myc regulates the transcription of the PRC2 gene to control the expression of developmental genes in embryonic stem cells. Mol Cell Biol. 32(4):840-51.

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