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EpiQuik Nuclear Extraction Kit


For isolation of nuclear proteins from cells or tissues while keeping enzymatic activity intact

Citations (181) | Write a Review
To demonstrate the quality of the materials and protocol of the EpiQuik Nuclear Extraction Kit, nuclear extracts were prepared from MCF-7 cells and the activity of HDACs were measured using different amounts of the extract. The result shown in the figure demonstrates the kit's high specificity.
Input Type: Unisolated Samples
Research Area: DNA Methylation, Histone Acetylation, Histone Methylation, Sumoylation
Target Application: Sample Isolation
Vessel Format: Columns/Tubes
100% Guarantee: 6 months
Catalog No.SizePriceQty
OP-0002-1100 assays $158.00 
Availability: Usually Ships in 1 Day or Same day delivery Same Day NY Delivery 
Product Overview

The EpiQuik™ Nuclear Extraction Kit is a complete set of optimized reagents to provide a simple and selective method for isolating nuclear proteins used for a variety of applications in just 60 minutes. These applications may include Western blot, protein-DNA binding assays, nuclear enzyme assays, and any other procedures requiring optimized nuclear proteins. The protocol is fast and easy-to-use, and also isolates very abundant yields of nuclear extract from mammalian cells or tissue samples. The kit is also specifically designed to meet the requirements of nuclear extracts used in various other epigenetic products from Epigentek, including DNMTHDAC, and HAT assays. Some of the kit's highlighted features include the following:

  • Pre-optimized and simple step-wise 1 hour protocol.
  • Conveniently includes all essential reagents to carry out a nuclear extraction.
  • Standardized procedure for reproducible results.

Principle & Procedure
The EpiQuik™ Nuclear Extraction Kit simply applies our proprietary nuclear protein isolation buffers to cell/tissues. After treatment with pre-lysis and lysis buffers, the nuclear proteins are easily extracted for immediate use or storage at proper conditions.

Starting Materials & Yield
A total of 100 standard extractions (using 10^7 cells or 20 mg tissues) can be isolated from mammalian cells or tissue samples with this kit. Total yield can be up to 100 µg per optimal extraction, although the results may somewhat vary depending on the cell or tissue type.

Product Components

NE1 (10X Pre-Extraction Buffer)
NE2 (Extraction Buffer)
PIC (1000X Protease Inhibitor Cocktail)
1000X DTT Solution
User Guide

Frequently Asked Q's

1. Should DTT be added into NE1 for tissue extraction?
It is not necessary.

2. How many rpm should be used for centrifuging the cell extract if using a desktop centrifuge?
12,000-14,000 rpm.

3. What should I do if the addition of 5 µl NE2 into 1 M of cells cannot suspend the nuclei?
Add more NE2. For example, add 10 µl to 1 M nuclei.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing along with your contact information and institution name.

[Material Safety Data Sheet]
Product Citations

Cantacorps L et. al. (September 2017). Maternal alcohol binge drinking induces persistent neuroinflammation associated with myelin damage and behavioural dysfunctions in offspring mice. Neuropharmacology. 123:368-384.

Liu X et. al. (July 2017). Laminarin protects against hydrogen peroxide-induced oxidative damage in MRC-5 cells possibly via regulating NRF2 Peerj. 5(e3642)

Senyildiz M et. al. (July 2017). Effects of BPA on global DNA methylation and global histone 3 lysine modifications in SH-SY5Y cells: An epigenetic mechanism linking the regulation of chromatin modifiying genes. Toxicol In Vitro.

Jesse Roman et. al. (July 2017). Epigenetic regulation of EC-SOD expression in aging lung fibroblasts: role of histone acetylation Free Radical Biology and Medicine.

Yong Seung Lee et. al. (July 2017). An ultra-effective method of generating extramultipotent cells from human fibroblasts by ultrasound Biomaterials. 143:65-78.

Shenoy N et. al. (July 2017). Upregulation of TET activity with ascorbic acid induces epigenetic modulation of lymphoma cells. Blood Cancer J. 7(7):e587.

Wang SB et. al. (June 2017). Early growth response factor 1 is essential for cigarette smoke-induced MUC5AC expression in human bronchial epithelial cells. Biochem Biophys Res Commun.

Wu Z et. al. (June 2017). Ultraviolet B inhibition of DNMT1 activity via AhR activation dependent SIRT1 suppression in CD4+ T cells from systemic lupus erythematosus patients. J Dermatol Sci. 86(3):230-237.

Kinner-Bibeau LB et. al. (May 2017). HSPs drive dichotomous T-cell immune responses via DNA methylome remodelling in antigen presenting cells. Nat Commun. 8:15648.

Oikawa S et. al. (May 2017). Various Regulatory Modes for Circadian Rhythmicity and Sexual Dimorphism in the Non-Neuronal Cardiac Cholinergic System. J Cardiovasc Transl Res.

Prasad R et. al. (May 2017). Honokiol inhibits ultraviolet radiation-induced immunosuppression through inhibition of ultraviolet-induced inflammation and DNA hypermethylation in mouse skin. Sci Rep. 7(1):1657.

Miousse IR et. al. (May 2017). Dose-response analysis of epigenetic, metabolic, and apical endpoints after short-term exposure to experimental hepatotoxicants. Food Chem Toxicol.

Yu T et. al. (May 2017). Metformin inhibits SUV39H1-mediated migration of prostate cancer cells. Oncogenesis. 6(5):e324.

Geyer KK et. al. (May 2017). The Biomphalaria glabrata DNA methylation machinery displays spatial tissue expression, is differentially active in distinct snail populations and is modulated by interactions with Schistosoma mansoni. PLoS Negl Trop Dis. 11(5):e0005246.

Wang Z et. al. (April 2017). Artesunate Suppresses the Growth of Prostatic Cancer Cells through Inhibiting Androgen Receptor. Biol Pharm Bull. 40(4):479-485.

Zhouwei Wu et. al. (March 2017). Ultraviolet B inhibition of DNMT1 activity via AhR activation dependent SIRT1 suppression in CD4+ T cells from systemic lupus erythematosus patients Journal of Dermatological Science.

Perreau-Lenz S et. al. (February 2017). mPer1 promotes morphine-induced locomotor sensitization and conditioned place preference via histone deacetylase activity. Psychopharmacology (Berl).

Seo JS et. al. (February 2017). Hinokitiol induces DNA demethylation via DNMT1 and UHRF1 inhibition in colon cancer cells. BMC Cell Biol. 18(1):14.

Yushang Cui et. al. (February 2017). Minichromosome maintenance complex facilitates the recruitment of BRCA1 onto chromatin and foci formation in A549 cells IJCEP. 10(2):1611-1618.

Zhu Y et. al. (January 2017). Enhanced neuroinflammation mediated by DNA methylation of the glucocorticoid receptor triggers cognitive dysfunction after sevoflurane anesthesia in adult rats subjected to maternal separation during the neonatal period. J Neuroinflammation. 14(1):6.

Wawrzyniak P et. al. (January 2017). Regulation of bronchial epithelial barrier integrity by type 2 cytokines and histone deacetylases in asthmatic patients. J Allergy Clin Immunol. 139(1):93-103.

Yang J et. al. (December 2016). Inhibition of KPNA4 attenuates prostate cancer metastasis. Oncogene.

Dwight A.L. Mattocks et. al. (December 2016). Methionine restriction protects C57BL6/J mice from age related increase in S-adenosylhomocysteine and global DNA hypomethylation in liver Science Direct. Experimental Gerontology

Ji J et. al. (October 2016). PPARβ/δ activation protects against corticosterone-induced ER stress in astrocytes by inhibiting the CpG hypermethylation of microRNA-181a. Neuropharmacology. 113(Pt A):396-406.

Kanwal R et. al. (September 2016). Dietary Flavones as Dual Inhibitors of DNA Methyltransferases and Histone Methyltransferases. PLoS One. 11(9):e0162956.

Zhang S et. al. (August 2016). Physiological and biochemical aspects of methionine isomers and a methionine analogue in broilers. Poult Sci.

Liu H et. al. (August 2016). Thymidine phosphorylase exerts complex effects on bone resorption and formation in myeloma. Sci Transl Med. 8(353):353ra113.

Meng M et. al. (August 2016). Novel immunostimulators with a thiazolidin-4-one ring promote the immunostimulatory effect of human iNKT cells on the stimulation of Th2-like immune responsiveness via GATA3 activation in vitro. Int Immunopharmacol. 39:353-358.

Xiaomei Wang et. al. (June 2016). Lipid reduces GLP-1 production via inhibiting NF-κB p65 and IL-6 in intestinal L cells and pancreatic alpha cells IJCEP. 9(6)

Ruebel M et. al. (June 2016). Maternal Obesity is Associated with Ovarian Inflammation and Up-regulation of Early Growth Response Factor (Egr)-1. Am J Physiol Endocrinol Metab. :ajpendo.00524.2015.

Herencia C et. al. (June 2016). Procaine Inhibits Osteo/Odontogenesis through Wnt/β-Catenin Inactivation. PLoS One. 11(6):e0156788.

Hu C et. al. (June 2016). Frontline Science: ATF3 is responsible for the inhibition of TNF-α release and the impaired migration of acute ethanol-exposed monocytes and macrophages. J Leukoc Biol.

Yang T et. al. (June 2016). Hepatocyte growth factor induced differentiation of bone mesenchymal stem cells towards hepatocyte-like cells occurs through nuclear factor-kappa B signaling in vitro. Cell Biol Int.

Nozik-Grayck E et. al. (May 2016). Histone deacetylation contributes to low extracellular superoxide dismutase expression in human idiopathic pulmonary arterial hypertension. Am J Physiol Lung Cell Mol Physiol. :ajplung.00263.2015.

Iglesias González T et. al. (May 2016). New strategy to address DNA-methyl transferase activity in ovarian cancer cell cultures by monitoring the formation of 5-methylcytosine using HPLC-UV. J Chromatogr B Analyt Technol Biomed Life Sci. 1028:16-24.

Navakauskienė et. al. (May 2016). Histone demethylating agents as potential S-adenosyl-L-methionine-competitors Med Chem Comm.

Gang Yu et. al. (April 2016). Ozone therapy could attenuate tubulointerstitial injury in adenine-induced CKD rats by mediating Nrf2 and NF-κB IJBMS. 19(10)

Muta K et. al. (March 2016). Curcumin ameliorates nephrosclerosis via suppression of histone acetylation independent of hypertension Nephrol Dial Transplant.

Iwanowycz S et. al. (March 2016). Emodin bi-directionally modulates macrophage polarization and epigenetically regulates macrophage memory. J Biol Chem.

Moriyama T et. al. (March 2016). Atrial natriuretic peptide attenuation of renal ischemia–reperfusion injury after major surgery J Surg Res. 201(1):213-218.

Huang F et. al. (March 2016). Interleukin‑1β increases the risk of gastric cancer through induction of aberrant DNA methylation in a mouse model Oncol Lett.

Zhang Y et. al. (February 2016). 2-Methoxyestradiol prevents monocyte adhesion to vascular endothelial cells via downregulation of VCAM-1 expression. Gynecol Endocrinol. :1-6.

Tang SC et. al. (January 2016). Glycolic Acid Silences Inflammasome Complex Genes, NLRC4 and ASC, by Inducing DNA Methylation in HaCaT Cells. DNA Cell Biol.

Li Y et. al. (January 2016). HBXIP and LSD1 Scaffolded by lncRNA Hotair Mediate Transcriptional Activation by c-Myc. Cancer Res. 76(2):293-304.

Zhang X et. al. (January 2016). Cell-free 3D scaffold with two-stage delivery of miRNA-26a to regenerate critical-sized bone defects. Nat Commun. 7:10376.

Sakamoto T et. al. (January 2016). A Histone Deacetylase Inhibitor Suppresses Epithelial-Mesenchymal Transition and Attenuates Chemoresistance in Biliary Tract Cancer. PLoS One. 11(1):e0145985.

Bott AJ et. al. (December 2015). Oncogenic Myc Induces Expression of Glutamine Synthetase through Promoter Demethylation. Cell Metab. 22(6):1068-77.

Yasuda H et. al. (November 2015). Exendin-4 promotes extracellular-superoxide dismutase expression in A549 cells through DNA demethylation J Clin Biochem Nutr.

Bhargavan B et. al. (November 2015). Toll-like receptor-3 mediates HIV-1 transactivation via NFκB and JNK pathways and histone acetylation, but prolonged activation suppresses Tat and HIV-1 replication. Cell Signal.

Moriyama T et. al. (November 2015). Atrial natriuretic peptide attenuation of renal ischemia/reperfusion injury after major surgery JSR.

Nie D et. al. (November 2015). Hepatitis C virus core protein interacts with Snail and histone deacetylases to promote the metastasis of hepatocellular carcinoma. Oncogene.

Melmaiee K et. al. (October 2015). Quantification and Gene Expression Analysis of Histone Deacetylases in Common Bean During Rust Fungal Inoculation IJG.

Artacho-Cordón F et. al. (October 2015). Valproic acid modulates radiation-enhanced matrix metalloproteinase activity and invasion of breast cancer cells. Int J Radiat Biol. :1-11.

Tomasi ML et. al. (September 2015). Methionine adenosyltransferase α2 sumoylation positively regulate Bcl-2 expression in human colon and liver cancer cells. Oncotarget.

Rodríguez-Miguel C et. al. (September 2015). The Role of Dietary Extra Virgin Olive Oil and Corn Oil on the Alteration of Epigenetic Patterns in the Rat DMBA-Induced Breast Cancer Model. PLoS One. 10(9):e0138980.

Li H et. al. (September 2015). Rapamycin restores p14, p15 and p57 expression and inhibits the mTOR/p70S6K pathway in acute lymphoblastic leukemia cells. Int J Hematol.

Granados ML et. al. (September 2015). Contributions of the Epidermal Growth Factor Receptor to Acquisition of Platinum Resistance in Ovarian Cancer Cells. PLoS One. 10(9):e0136893.

Miousse IR et. al. (September 2015). In Vitro Toxicity and Epigenotoxicity of Different Types of Ambient Particulate Matter. Toxicol Sci.

Kang SC et. al. (August 2015). Transcriptomic Profiling and H3K27me3 Distribution Reveal Both Demethylase-Dependent and Independent Regulation of Developmental Gene Transcription in Cell Differentiation. PLoS One. 10(8):e0135276.

Miao Z et. al. (August 2015). Negative Effects of SRD5A1 on Nuclear Activity of Progesterone Receptor Isoform B in JEG3 Cells. Reprod Sci.

Pushpakumar S et. al. (July 2015). DNA hypermethylation in hyperhomocysteinemia contributes to abnormal extracellular matrix metabolism in the kidney. FASEB J.

Zhang QQ et. al. (June 2015). Cathepsin L suppression increases the radiosensitivity of human glioma U251 cells via G<sub>2</sub>/M cell cycle arrest and DNA damage. Acta Pharmacol Sin.

Boosani CS et. al. (June 2015). Down-regulation of hsa-miR-1264 contributes to DNMT1-mediated silencing of SOCS3. Mol Biol Rep.

Li JJ et. al. (June 2015). Homocysteine Triggers Inflammatory Responses in Macrophages through Inhibiting CSE-H2S Signaling via DNA Hypermethylation of CSE Promoter. Int J Mol Sci. 16(6):12560-12577.

Srinivasan A et. al. (May 2015). Quercetin regulates β-catenin signaling and reduces the migration of triple negative breast cancer. Mol Carcinog.

Nugent BM et. al. (May 2015). Brain feminization requires active repression of masculinization via DNA methylation. Nat Neurosci. 18(5):690-7.

Mei Y et. al. (April 2015). Aging-associated formaldehyde-induced norepinephrine deficiency contributes to age-related memory decline. Aging Cell.

Yang H et. al. (April 2015). Lipoprotein lipase deficiency leads to α-synuclein aggregation and ubiquitin C-terminal hydrolase L1 reduction. Neuroscience. 290:1-10.

Shan W et. al. (April 2015). LukS-PV, a component of Panton-Valentine leukocidin, exerts potent activity against acute myeloid leukemia in vitro and in vivo. Int J Biochem Cell Biol. 61:20-8.

Khan MA et. al. (April 2015). (-)-Epigallocatechin-3-gallate reverses the expression of various tumor-suppressor genes by inhibiting DNA methyltransferases and histone deacetylases in human cervical cancer cells. Oncol Rep. 33(4):1976-84.

Liu C et. al. (February 2015). PAQR3 modulates H3K4 trimethylation by spatial modulation of the regulatory subunits of COMPASS-like complexes in mammalian cells. Biochem J.

Hatano R et. al. (February 2015). CD26-Mediated Induction of EGR2 and IL-10 as Potential Regulatory Mechanism for CD26 Costimulatory Pathway. J Immunol. 194(3):960-72.

Zhang Y et. al. (February 2015). Green tea polyphenol EGCG reverse cisplatin resistance of A549/DDP cell line through candidate genes demethylation Biomedicine & Pharmacotherapy. 69:285–290. Abstract

Fassl SK et. al. (January 2015). Transcriptome Assessment Reveals a Dominant Role for TLR4 in the Activation of Human Monocytes by the Alarmin MRP8. J Immunol. 194(2):575-83.

Wang B et. al. (January 2015). Exposure to low dose cadmium enhances FL83B cells proliferation through down-regulation of caspase-8 by DNA hypermethylation Toxicol. Res.. (Advance Article) Abstract

Austermann J et. al. (December 2014). Alarmins MRP8 and MRP14 Induce Stress Tolerance in Phagocytes under Sterile Inflammatory Conditions. Cell Rep. 9(6):2112-23.

Sanders YY et. al. (December 2014). Epigenetic mechanisms regulate NADPH oxidase-4 expression in cellular senescence. Free Radic Biol Med. 79C:197-205.

Cardenas H et. al. (November 2014). TGF-β induces global changes in DNA methylation during the epithelial-to-mesenchymal transition in ovarian cancer cells. Epigenetics.

Lai J et. al. (August 2014). Transcriptional regulation of the p73 gene by Nrf-2 and promoter CpG methylation in human breast cancer. Oncotarget. 5(16):6909-22.

Korenaga M et. al. (August 2014). Branched-chain amino acids reduce hepatic iron accumulation and oxidative stress in hepatitis C virus polyprotein-expressing mice. Liver Int.

Lai J et. al. (August 2014). TAp73 and ΔNp73 Have Opposing Roles in 5-aza-2'-Deoxycytidine-Induced Apoptosis in Breast Cancer Cells. Mol Cells.

Sahdeo S et. al. (August 2014). Dyclonine rescues frataxin deficiency in animal models and buccal cells of patients with Friedreich's ataxia. Hum Mol Genet.

Desplats P et. al. (July 2014). Epigenetic Alterations in the Brain Associated with HIV-1 Infection and Methamphetamine Dependence. PLoS One. 9(7):e102555.

Olsvik PA et. al. (July 2014). Impact of dietary selenium on methylmercury toxicity in juvenile Atlantic cod: A transcriptional survey. Chemosphere. 120C:199-205.

Yu HR et. al. (June 2014). Prenatal dexamethasone exposure in rats results in long-term epigenetic histone modifications and tumor necrosis factor-α production decrease. Immunology.

Zhang W et. al. (May 2014). Prenatal alcohol exposure causes the over-expression of DHAND and EHAND by increasing histone H3K14 acetylation in C57 BL/6 mice. Toxicol Lett. 228(3):140-146.

Sanders YY et. al. (May 2014). Histone deacetylase inhibition promotes fibroblast apoptosis and ameliorates pulmonary fibrosis in mice. Eur Respir J. 43(5):1448-58.

Scola G et. al. (April 2014). Lithium reduces the effects of rotenone-induced complex I dysfunction on DNA methylation and hydroxymethylation in rat cortical primary neurons. Psychopharmacology (Berl).

Dhliwayo N et. al. (April 2014). Parp inhibition prevents ten eleven translocase enzyme activation and hyperglycemia induced DNA demethylation. Diabetes.

He W et. al. (April 2014). Anticancer cardamonin analogs suppress the activation of NF-kappaB pathway in lung cancer cells. Mol Cell Biochem. 389(1-2):25-33.

Chaturvedi P et. al. (April 2014). Differential regulation of DNA methylation versus histone acetylation in cardiomyocytes during HHcy in vitro and in vivo: an epigenetic mechanism. Physiol Genomics. 46(7):245-55.

Yamashita K et. al. (March 2014). The effects of the heme precursor 5-aminolevulinic acid (ALA) on REV-ERBα activation. FEBS Open Bio. 4:347-52.

Maiti AK et. al. (March 2014). Combined protein- and nucleic acid-level effects of rs1143679 (R77H), a lupus-predisposing variant within ITGAM. Hum Mol Genet.

Wang Y et. al. (March 2014). Ras-induced epigenetic inactivation of Ras-related associated with diabetes gene (RRAD) promotes glucose uptake in a human ovarian cancer model. J Biol Chem.

Monteiro JB et. al. (March 2014). Endometriosis is characterized by a distinct pattern of histone 3 and histone 4 lysine modifications. Reprod Sci. 21(3):305-18.

Kalani A et. al. (February 2014). Nutri-epigenetics ameliorates blood-brain barrier damage and neurodegeneration in hyperhomocysteinemia: role of folic acid. J Mol Neurosci. 52(2):202-15.

Gómez-Sánchez R et. al. (February 2014). Mitochondrial impairment increases FL-PINK1 levels by calcium-dependent gene expression. Neurobiol Dis. 62:426-40.

Zhu X et. al. (January 2014). BCR-ABL1-positive microvesicles transform normal hematopoietic transplants through genomic instability: implications for donor cell leukemia. Leukemia.

Chen Z et. al. (January 2014). Regulation of c-kit+ progenitor cells by stromal cell derived factor-1α in adult murine heart. Heart Lung Circ. 23(1):75-81.

Watanabe R et. al. (December 2013). Inhibition of NF-κB activation by a novel IKK inhibitor reduces the severity of experimental autoimmune myocarditis via suppression of T-cell activation. Am J Physiol Heart Circ Physiol. 305(12):H1761-71.

Liu L et. al. (November 2013). Histone lysine-specific demethylase 1 (LSD1) protein is involved in Sal-like protein 4 (SALL4)-mediated transcriptional repression in hematopoietic stem cells. J Biol Chem. 288(48):34719-28.

Moldrich RX et. al. (November 2013). Inhibition of histone deacetylase in utero causes sociability deficits in postnatal mice. Behav Brain Res. 257:253-64.

Armstrong VL et. al. (November 2013). Expression of DNA Methyltransferases Is Influenced by Growth Hormone in the Long-Living Ames Dwarf Mouse In Vivo and In Vitro. J Gerontol A Biol Sci Med Sci.

Ding Z et. al. (November 2013). Propofol administration modulates AQP-4 expression and brain edema after traumatic brain injury. Cell Biochem Biophys. 67(2):615-22.

Li C et. al. (November 2013). The immunostimulatory effect of novel immunostimulator CH2b with a thiazolidin-4-one ring on the functions of LPS-activated RAW 264.7 macrophages in vitro. Int Immunopharmacol. 17(3):698-703.

Kittan NA et. al. (October 2013). Cytokine induced phenotypic and epigenetic signatures are key to establishing specific macrophage phenotypes. PLoS One. 8(10):e78045.

Ge Y et. al. (October 2013). Inhibition of monomethylarsonous acid (MMA(III))-induced cell malignant transformation through restoring dysregulated histone acetylation. Toxicology. 312:30-5.

Bradley-Whitman MA et. al. (October 2013). Epigenetic changes in the progression of Alzheimer's disease. Mech Ageing Dev. 134(10):486-95.

Zhang X et. al. (September 2013). Histone deacetylase inhibition downregulates collagen 3A1 in fibrotic lung fibroblasts. Int J Mol Sci. 14(10):19605-17.

Herrera I et. al. (September 2013). Matrix metalloproteinase (MMP)-1 induces lung alveolar epithelial cell migration and proliferation, protects from apoptosis, and represses mitochondrial oxygen consumption. J Biol Chem. 288(36):25964-75.

Wu Z et. al. (September 2013). Ultraviolet B enhances DNA hypomethylation of CD4+ T cells in systemic lupus erythematosus via inhibiting DNMT1 catalytic activity. J Dermatol Sci. 71(3):167-73.

Huang FY et. al. (August 2013). Characterization of interleukin-1β in Helicobacter pylori-induced gastric inflammation and DNA methylation in interleukin-1 receptor type 1 knockout (IL-1R1(-/-)) mice. Eur J Cancer. 49(12):2760-70.

Bu S et. al. (August 2013). LukS-PV induces mitochondrial-mediated apoptosis and G0/G1 cell cycle arrest in human acute myeloid leukemia THP-1 cells. Int J Biochem Cell Biol. 45(8):1531-7.

Wu L et. al. (August 2013). Altered methylation of IGF2 DMR0 is associated with neural tube defects. Mol Cell Biochem. 380(1-2):33-42.

Zhang X et. al. (August 2013). Upregulated miR-155 in papillary thyroid carcinoma promotes tumor growth by targeting APC and activating Wnt/β-catenin signaling. J Clin Endocrinol Metab. 98(8):E1305-13.

Williams-Karnesky RL et. al. (August 2013). Epigenetic changes induced by adenosine augmentation therapy prevent epileptogenesis. J Clin Invest. 123(8):3552-63.

Sunahori K et. al. (July 2013). The catalytic subunit of protein phosphatase 2A (PP2Ac) promotes DNA hypomethylation by suppressing the phosphorylated mitogen-activated protein kinase/extracellular signal-regulated kinase (ERK) kinase (MEK)/phosphorylated ERK/DNMT1 protein pathway in T-cells from controls and systemic lupus erythematosus patients. J Biol Chem. 288(30):21936-44.

Chen Z et. al. (July 2013). Epigenetic regulation of cardiac progenitor cells marker c-kit by stromal cell derived factor-1α. PLoS One. 8(7):e69134.

Shyamasundar S et. al. (June 2013). Analysis of epigenetic factors in mouse embryonic neural stem cells exposed to hyperglycemia. PLoS One. 8(6):e65945.

Soda K et. al. (May 2013). Increased polyamine intake inhibits age-associated alteration in global DNA methylation and 1,2-dimethylhydrazine-induced tumorigenesis. PLoS One. 8(5):e64357.

Yamada D et. al. (May 2013). Role of crosstalk between interleukin-6 and transforming growth factor-beta 1 in epithelial-mesenchymal transition and chemoresistance in biliary tract cancer. Eur J Cancer. 49(7):1725-40.

Li Y et. al. (April 2013). Acute myocardial infarction induced functional cardiomyocytes to re-enter the cell cycle. Am J Transl Res. 5(3):327-35.

Kauntz H et. al. (April 2013). Epigenetic effects of the natural flavonolignan silibinin on colon adenocarcinoma cells and their derived metastatic cells. Oncol Lett. 5(4):1273-1277.

Sun L et. al. (March 2013). The promoter region of interferon-gamma is hypermethylated in neonatal foals and its demethylation is associated with increased gene expression. Dev Comp Immunol. 39(3):273-8.

Delaney C et. al. (February 2013). Maternal diet supplemented with methyl-donors protects against atherosclerosis in F1 ApoE(-/-) mice. PLoS One. 8(2):e56253.

Molineros JE et. al. (February 2013). Admixture mapping in lupus identifies multiple functional variants within IFIH1 associated with apoptosis, inflammation, and autoantibody production. PLoS Genet. 9(2):e1003222.

Kim G et. al. (February 2013). A novel role for IL-27 in mediating the survival of activated mouse CD4 T lymphocytes. J Immunol. 190(4):1510-8.

Kano Y et. al. (February 2013). Suppression of LFA-1 expression by spermine is associated with enhanced methylation of ITGAL, the LFA-1 promoter area. PLoS One. 8(2):e56056.

Sanders YY et. al. (January 2013). Histone modifications in senescence-associated resistance to apoptosis by oxidative stress. Redox Biol. 1(1):8-16.

Metes-Kosik N et. al. (December 2012). Both selenium deficiency and modest selenium supplementation lead to myocardial fibrosis in mice via effects on redox-methylation balance. Mol Nutr Food Res. 56(12):1812-24.

D'Costa ZJ et. al. (November 2012). Transcriptional repression of E-cadherin by human papillomavirus type 16 E6. PLoS One. 7(11):e48954.

Ramani K et. al. (October 2012). Mechanism and significance of changes in glutamate-cysteine ligase expression during hepatic fibrogenesis. J Biol Chem. 287(43):36341-55.

Horiuchi A et. al. (October 2012). Hypoxia upregulates ovarian cancer invasiveness via the binding of HIF-1α to a hypoxia-induced, methylation-free hypoxia response element of S100A4 gene. Int J Cancer. 131(8):1755-67.

Huang FY et. al. (October 2012). Helicobacter pylori induces promoter methylation of E-cadherin via interleukin-1β activation of nitric oxide production in gastric cancer cells. Cancer. 118(20):4969-80.

Desplats P et. al. (September 2012). α-Synuclein induces alterations in adult neurogenesis in Parkinson disease models via p53-mediated repression of Notch1. J Biol Chem. 287(38):31691-702.

Zopf S et. al. (September 2012). Inhibition of DNA methyltransferase activity and expression by treatment with the pan-deacetylase inhibitor panobinostat in hepatocellular carcinoma cell lines. BMC Cancer. 12:386.

Yamawaki Y et. al. (September 2012). Antidepressant-like effect of sodium butyrate (HDAC inhibitor) and its molecular mechanism of action in the rat hippocampus. World J Biol Psychiatry. 13(6):458-67.

Babu PV et. al. (September 2012). Epigallocatechin gallate reduces vascular inflammation in db/db mice possibly through an NF-κB-mediated mechanism. Mol Nutr Food Res. 56(9):1424-32.

Galoian K et. al. (June 2012). mTORC1 inhibition and ECM-cell adhesion-independent drug resistance via PI3K-AKT and PI3K-RAS-MAPK feedback loops. Tumour Biol. 33(3):885-90.

Begum G et. al. (April 2012). Epigenetic changes in fetal hypothalamic energy regulating pathways are associated with maternal undernutrition and twinning. FASEB J. 26(4):1694-703.

Maeda R et. al. (March 2012). Immunostimulatory activity of polysaccharides isolated from Caulerpa lentillifera on macrophage cells. Biosci Biotechnol Biochem. 76(3):501-5.

Samudio-Ruiz SL et. al. (March 2012). Increased DNA methyltransferase activity and DNA methylation following Epidermal Growth Factor stimulation in ovarian cancer cells. Epigenetics. 7(3):216-24.

Neri F et. al. (February 2012). Myc regulates the transcription of the PRC2 gene to control the expression of developmental genes in embryonic stem cells. Mol Cell Biol. 32(4):840-51.

Ponomarev I et. al. (February 2012). Gene coexpression networks in human brain identify epigenetic modifications in alcohol dependence. J Neurosci. 32(5):1884-97.

Trisciuoglio D et. al. (January 2012). CPTH6, a thiazole derivative, induces histone hypoacetylation and apoptosis in human leukemia cells. Clin Cancer Res. 18(2):475-86.

Yang J et. al. (January 2012). Stem cell gene SALL4 suppresses transcription through recruitment of DNA methyltransferases. J Biol Chem. 287(3):1996-2005.

Shu L et. al. (December 2011). Epigenetic CpG demethylation of the promoter and reactivation of the expression of Neurog1 by curcumin in prostate LNCaP cells. AAPS J. 13(4):606-14.

Geyer KK et. al. (August 2011). Cytosine methylation regulates oviposition in the pathogenic blood fluke Schistosoma mansoni. Nat Commun. 2:424.

Cai H et. al. (August 2011). Inhibition of melanoma cell proliferation by targeting Wnt/β-catenin pathway through Sox4 RNA interference. J Huazhong Univ Sci Technolog Med Sci. 31(4):565-9.

Huang L et. al. (August 2011). Prevention of transcriptional silencing by a replicator-binding complex consisting of SWI/SNF, MeCP1, and hnRNP C1/C2. Mol Cell Biol. 31(16):3472-84.

Dusio GF et. al. (July 2011). Stimulation of TLRs by LMW-HA induces self-defense mechanisms in vaginal epithelium. Immunol Cell Biol. 89(5):630-9.

Nocchi L et. al. (June 2011). Thrombomodulin is silenced in malignant mesothelioma by a poly(ADP-ribose) polymerase-1-mediated epigenetic mechanism. J Biol Chem. 286(22):19478-88.

Fritzenwanger M et. al. (June 2011). Impact of short-term systemic hypoxia on phagocytosis, cytokine production, and transcription factor activation in peripheral blood cells. Mediators Inflamm. 2011:429501.

Jin Y et. al. (June 2011). Homocysteine levels impact directly on epigenetic reprogramming in astrocytes. Neurochem Int. 58(7):833-8.

Shi YY et. al. (April 2011). Diet and cell size both affect queen-worker differentiation through DNA methylation in honey bees (Apis mellifera, Apidae). PLoS One. 6(4):e18808.

Nandakumar V et. al. (April 2011). (-)-Epigallocatechin-3-gallate reactivates silenced tumor suppressor genes, Cip1/p21 and p16INK4a, by reducing DNA methylation and increasing histones acetylation in human skin cancer cells. Carcinogenesis. 32(4):537-44.

Kawakami K et. al. (January 2011). Long interspersed nuclear element-1 hypomethylation is a potential biomarker for the prediction of response to oral fluoropyrimidines in microsatellite stable and CpG island methylator phenotype-negative colorectal cancer. Cancer Sci. 102(1):166-74.

Herrera F et. al. (December 2010). Synergistic effect of retinoic acid and cytokines on the regulation of glial fibrillary acidic protein expression. J Biol Chem. 285(50):38915-22.

Sui L et. al. (December 2010). Effects of perinatal hypothyroidism on regulation of reelin and brain-derived neurotrophic factor gene expression in rat hippocampus: Role of DNA methylation and histone acetylation. Steroids. 75(12):988-97.

Skowronski K et. al. (August 2010). Ischemia dysregulates DNA methyltransferases and p16INK4a methylation in human colorectal cancer cells. Epigenetics. 5(6):547-56.

Kawada S et. al. (August 2010). Increased oxygen tension attenuates acute ultraviolet-B-induced skin angiogenesis and wrinkle formation. Am J Physiol Regul Integr Comp Physiol. 299(2):R694-701.

Hu B et. al. (July 2010). Epigenetic regulation of myofibroblast differentiation by DNA methylation. Am J Pathol. 177(1):21-8.

Rugg-Gunn PJ et. al. (June 2010). Distinct histone modifications in stem cell lines and tissue lineages from the early mouse embryo. Proc Natl Acad Sci U S A. 107(24):10783-90.

Pandey M et. al. (June 2010). Promoter demethylation and chromatin remodeling by green tea polyphenols leads to re-expression of GSTP1 in human prostate cancer cells. Int J Cancer. 126(11):2520-33.

Chen F et. al. (June 2010). Influence of silencing TRAF6 with shRNA on LPS/TLR4 signaling in vitro. J Huazhong Univ Sci Technolog Med Sci. 30(3):278-84.

Kastl L et. al. (April 2010). Effects of decitabine on the expression of selected endogenous control genes in human breast cancer cells. Mol Cell Probes. 24(2):87-92.

Fritzenwanger M et. al. (March 2010). Cardiotrophin-1 induces tumor necrosis factor alpha synthesis in human peripheral blood mononuclear cells. Mediators Inflamm. 2009:489802.

Nishihara M et. al. (February 2010). Transcriptional regulation of the human establishment of cohesion 1 homolog 2 gene. Biochem Biophys Res Commun. 393(1):111-7.

Perisic T et. al. (February 2010). Valproate and amitriptyline exert common and divergent influences on global and gene promoter-specific chromatin modifications in rat primary astrocytes. Neuropsychopharmacology. 35(3):792-805.

Majid S et. al. (January 2010). Genistein reverses hypermethylation and induces active histone modifications in tumor suppressor gene B-Cell translocation gene 3 in prostate cancer. Cancer. 116(1):66-76.

Duthie SJ et. al. (January 2010). Folate deficiency alters hepatic and colon MGMT and OGG-1 DNA repair protein expression in rats but has no effect on genome-wide DNA methylation. Cancer Prev Res (Phila). 3(1):92-100.

Fujishiro H et. al. (December 2009). Involvement of DNA hypermethylation in down-regulation of the zinc transporter ZIP8 in cadmium-resistant metallothionein-null cells. Toxicol Appl Pharmacol. 241(2):195-201.

Watson JA et. al. (October 2009). Generation of an epigenetic signature by chronic hypoxia in prostate cells. Hum Mol Genet. 18(19):3594-604.

Zhang XY et. al. (June 2009). Promoter cloning and characterization of the rabbit BK channel beta1 subunit gene. Gene. 438(1-2):33-9.

Nakamura H et. al. (May 2009). Role of angiotensin II type 1 receptor on retinal vascular leakage in a rat oxygen-induced retinopathy model. Ophthalmic Res. 41(4):210-5.

Liu Z et. al. (May 2009). Modulation of DNA methylation by a sesquiterpene lactone parthenolide. J Pharmacol Exp Ther. 329(2):505-14.

Majid S et. al. (April 2009). BTG3 tumor suppressor gene promoter demethylation, histone modification and cell cycle arrest by genistein in renal cancer. Carcinogenesis. 30(4):662-70.

Wang X et. al. (January 2009). RAF may induce cell proliferation through hypermethylation of tumor suppressor gene promoter in gastric epithelial cells. Cancer Sci. 100(1):117-25.

Fritzenwanger M et. al. (December 2008). Cardiotrophin-1 induces intercellular adhesion molecule-1 expression by nuclear factor kappaB activation in human umbilical vein endothelial cells. Chin Med J (Engl). 121(24):2592-8.

Roll JD et. al. (January 2008). DNMT3b overexpression contributes to a hypermethylator phenotype in human breast cancer cell lines. Mol Cancer. 7:15.

Miyazaki T et. al. (October 2007). E-cadherin gene promoter hypermethylation in H. pylori-induced enlarged fold gastritis. Helicobacter. 12(5):523-31.

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