Back to School 15% Off Back to School Sale! Take 15% Off Your Entire Order When Using the Code E1808BTS. » Learn More
Epigentek Home
EpiGentek Guarantee

EpiQuik Nuclear Extraction Kit II (Nucleic Acid-Free)


For isolation of nucleic acid-free nuclear proteins from cells or tissues

Citations (10) | Write a Review
Schematic image of the EpiQuik™ Nuclear Extraction Kit II (Nucleic Acid-Free).
Input Type: Unisolated Samples
Research Area: DNA Methylation, Histone Acetylation, Histone Methylation, Sumoylation
Target Application: Sample Isolation
Vessel Format: Columns/Tubes
100% Guarantee: 6 months
Catalog No.SizePriceQty
OP-0022-100100 extractions $162.00 
Availability: Usually Ships in 1 Day or Same day delivery Same Day NY Delivery 
Product Overview

The EpiQuik™ Nuclear Extraction Kit II (Nucleic Acid-Free) is a complete set of optimized reagents to provide a simple and selective method for isolating nuclear proteins free of nucleic acids attached, to be used in a variety of applications in just 60 minutes. These applications may include Western blot, protein-DNA binding assays, nuclear enzyme assays, and any other procedures requiring optimized nucleic acid-free nuclear proteins but not enzymatic activity. The protocol is fast and easy-to-use, and also isolates very abundant yields of nuclear extract from mammalian cells or tissue samples. Some of the kit's highlighted featured include the following:

  • Pre-optimized and simple step-wise 1 hour protocol.
  • Conveniently includes all essential reagents to carry out a nucleic acid-free nuclear extraction.
  • Standardized procedure for reproducible results.

Principle & Procedure
The EpiQuik™ Nuclear Extraction Kit II (Nucleic Acid-Free) simply applies our proprietary nuclear protein isolation buffers to cell/tissues. After treatment with pre-lysis and lysis buffers, the nucleic acid-free nuclear proteins are easily extracted for immediate use or storage at proper conditions.

Note: This item was formerly catalog no. OP-0002-2 (OP00022, OP-00022, OP0002-2)


Fig. 1. Schematic image of the EpiQuik™ Nuclear Extraction Kit II (Nucleic Acid-Free).

Product Components

NP1 (10X Pre-Extraction Buffer)
NP2 (Extraction Buffer)
NP3 (Extraction Pre-Cleaner)
NP4 (Extraction Cleaner)
1000X DTT Solution
1000X Protease Inhibitor Cocktail (PIC)
User Guide

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing along with your contact information and institution name.

[Material Safety Data Sheet]
Product Citations

Liu Y et. al. (July 2018). Integrated regulation of class II human endogenous retroviruses in a breast cancer cell line The Proceedings of the International Conference on Creationism. 8(16):191–199.

Yuyan Tan et. al. (April 2018). Upregulation of HDAC2 in Laser Capture Nigral Microglia in Parkinson’s Disease. Neurobiology of Aging.

Yana H et. al. (December 2015). The effects of LSD1 inhibition on self-renewal and differentiation of human induced pluripotent stem cells Exp Cell Res.

Haupt S et. al. (November 2015). Alternative promoter methylation regulates together with Foxp3 transcription of GARP. Biochim Biophys Acta.

Ivankovic D et. al. (October 2015). Mitochondrial and lysosomal biogenesis are activated following PINK1/parkin-mediated mitophagy. J Neurochem.

Wessam F. El-Hadidy et. al. (April 2014). Possible protective effect of procainamide as an epigenetic modifying agent in experimentally induced type 2 diabetes mellitus in rats. Alexandria Journal of Medicine. S2090-5068(14):00028-1. Abstract

Siuda D et. al. (April 2014). Social isolation-induced epigenetic changes in midbrain of adult mice. J Physiol Pharmacol. 65(2):247-55.

Yamashita K et. al. (March 2014). The effects of the heme precursor 5-aminolevulinic acid (ALA) on REV-ERBα activation. FEBS Open Bio. 4:347-52.

Wei W et. al. (May 2013). Codelivery of mTERT siRNA and paclitaxel by chitosan-based nanoparticles promoted synergistic tumor suppression. Biomaterials. 34(15):3912-23.

Galoian K et. al. (June 2012). mTORC1 inhibition and ECM-cell adhesion-independent drug resistance via PI3K-AKT and PI3K-RAS-MAPK feedback loops. Tumour Biol. 33(3):885-90.

Epigenetic haiku contest
Related Products

Researchers Also Purchased
EpiQuik Total Histone Extraction Kit
EpiQuik Total Histone Extraction Kit
EpiQuik DNMT Activity/Inhibition Assay Ultra Kit (Colorimetric)
EpiQuik DNMT Activity/Inhibition Assay Ultra Kit (Colorimetric)
EpiQuik Nuclear Extraction Kit
EpiQuik Nuclear Extraction Kit
EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit
EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit
Epigentek is an epigenetics company that specializes in epigenetic kits, antibodies, reagents, and services for epigenetic research in DNA methylation, histone modification, and chromatin studies. Terms & Conditions | Privacy Policy | Site Map
Copyright © 2017 Epigentek Group Inc. All rights reserved.