The EpiQuik™ Nuclear Extraction Kit is a complete set of optimized reagents to provide a simple and selective method for isolating nuclear proteins used for a variety of applications in just 60 minutes. These applications may include Western blot, protein-DNA binding assays, nuclear enzyme assays, and any other procedures requiring optimized nuclear proteins. The protocol is fast and easy-to-use, and also isolates very abundant yields of nuclear extract from mammalian cells or tissue samples. The kit is also specifically designed to meet the requirements of nuclear extracts used in various other epigenetic products from Epigentek, including DNMT, HDAC, and HAT assays. Some of the kit's highlighted features include the following:
- Pre-optimized and simple step-wise 1 hour protocol.
- Conveniently includes all essential reagents to carry out a nuclear extraction.
- Standardized procedure for reproducible results.
Principle & Procedure
The EpiQuik™ Nuclear Extraction Kit simply applies our proprietary nuclear protein isolation buffers to cell/tissues. After treatment with pre-lysis and lysis buffers, the nuclear proteins are easily extracted for immediate use or storage at proper conditions.
Starting Materials & Yield
A total of 100 standard extractions (using 10^7 cells or 20 mg tissues) can be isolated from mammalian cells or tissue samples with this kit. Total yield can be up to 100 µg per optimal extraction, although the results may somewhat vary depending on the cell or tissue type.
NE1 (10X Pre-Extraction Buffer)
NE2 (Extraction Buffer)
PIC (1000X Protease Inhibitor Cocktail)
1000X DTT Solution