We recommend using a newer version of this product: EpiQuik DNMT Activity/Inhibition ELISA Easy Kit (Colorimetric)
The EpiQuik™ DNA Methyltransferase Activity/Inhibition Assay Kit is a complete set of optimized buffers and reagents that allows the experimenter to measure DNA methyltransferase activity or inhibition at extremely fast speeds. The kit is ready-to-use and provides all the essential components needed to carry out a successful DNMT activity/inhibition experiment without the need for radioactivity or any special equipment. The EpiQuik™ DNA Methyltransferase Activity/Inhibition Assay Kit is suitable for measuring DNMT activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria. The kit has the following advantages:
- Very rapid procedure, which can be completed within 3 hours.
- Safe and innovative colorimetric assay without radioactivity, extraction, and chromatography.
- Strip microplate format makes the assay flexible: manual or high throughput analysis.
- Extremely simple, reliable, and consistent assay conditions.
Principle & Procedure
The EpiQuik™ DNA Methyltransferase Activity/Inhibition Assay Kit is designed for measuring total DNMT activity (de novo, maintenance). In an assay with this kit, the unique cytosine-rich DNA substrate is stably coated on the strip wells. These wells are specifically treated to have a high DNA absorption ability. DNMT enzymes transfer a methyl group to cytosine from Adomet to methylate DNA substrate. The methylated DNA can be recognized with an anti-5-methylcytosine antibody. The ratio or amount of methylated DNA, which is proportional to enzyme activity, can then be colorimetrically quantified through an ELISA-like reaction.
M1 (10X Wash Buffer)
M2 (DNMT Assay Buffer)
M3 (Adomet, 8 mM)*
M4 (DNMT Positive Control)*
M5 (Capture Antibody)*
M6 (Detection Antibody, 200 µg/ml)*
M7 (Developing Solution)
M8 (Stop Solution)
8-Well Substrate-Coated Strip (with Frame)
*For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.