EpiQuik HDAC Activity/Inhibition Assay Kit (Colorimetric)

We recommend using a newer version of this product: Epigenase HDAC Activity/Inhibition Direct Assay Kit (Colorimetric)

The EpiQuik™ HDAC Activity/Inhibition Assay Kit is a ready-to-use set of essential components needed to measure HDAC activity/inhibition safely and quickly. It is suitable for measuring HDAC activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria.

WHY CHOOSE THE EPIQUIK™ HDAC ACTIVITY/INHIBITION ASSAY KIT (COLORIMETRIC),

• Very quick procedure, which can be completed within 3 hours.
• Innovative colorimetric assay without radioactivity, extraction, and chromatography.
• Direct measurement of HDAC activity and inhibition without the use of lysyl endopeptidase, thereby avoiding the false inhibitory effect on HDACs.
• Strip microplate format makes the assay flexible in both manual and high throughput analysis formats.
• Simple, reliable, and consistent assay conditions.

The EpiQuik™ HDAC Activity/Inhibition Assay Kit is designed for measuring total HDAC activity/inhibition. In an assay with this kit, the unique acetylated histone substrate is stably captured on the strip wells. Active HDACs bind to and deacetylate histone substrate. The remaining un-deacetylated substrate can be recognized with a high affinity acetylated histone antibody. The ratio or amount of the un-deacetylated histone, which is inversely proportional to HDAC enzyme activity, can then be colorimetrically quantified through an ELISA-like reaction.

SCHEMATIC PROCEDURE: