Transcriptome-Specific RNA Methylation

Established methods used for epitranscriptome-wide m⁶A mapping like MeRIP-seq, PA-m⁶A-seq, and miCLIP have either been widely used but are unable to achieve high resolution in m⁶A profiling, or improve the profiling resolution but suffer from poor reproducibility and a complicated process. In particular, they are time-consuming (>2 days) and costly.

To address these issues, EpigenTek has developed a new method: CUT&RUN m⁶A MeRIP (Cleavage Under Target and Recover Using Nuclease for m⁶A enrichment). This innovative approach combines the advantages of MeRIP-seq and miCLIP with EpigenTek’s proprietary EpiQuik technology for:

• Higher Enrichment
• Lower Input
• Reduced Background
• Faster More Streamlined Procedure

CUT and RUN m⁶A MeRIP uses a state-of-the-art RNA cleavage enzyme mix to simultaneously fragment immunocaptured RNA and cleave/remove any RNA sequences in both ends of the target m⁶A -containing sequences, without affecting RNA regions occupied by the antibody. Short RNA fragments are consequently generated only bound with anti- m⁶A antibody. True target m⁶A -enriched regions can therefore be reliably identified, and high-resolution mapping achieved.

For 5mC RNA analysis, EpigenTek offers a complete set of optimized reagents for rapid RNA bisulfite conversion, as well as a convenient all-in-one kit that couples RNA bisulfite conversion with post-bisulfite library preparation for Illumina-compatible bisulfite sequencing.