Reduced Representation Bisulfite Sequencing (RRBS) Service


Reduced Representation Bisulfite Sequencing (RRBS) is an enhanced bisulfite-based sequencing method utilized to detect up 7 – 8 million unique CpG sites, covering nearly all CpG islands, gene promoters, and most genetic regulatory elements, gene bodies, and repeated DNA sequences.

RRBS is an NGS platform used to analyze the genome-wide methylation at base resolution detail by the use of a restriction enzyme and bisulfite treatment to focus on the areas in the genome that have a high CpG content. This is typically designed for researchers who are looking for cost effective ways to perform the sequencing instead of having Whole Genome Bisulfite Sequencing (WGBS) performed.

RRBS is typically recommended for first time users with bisulfite sequencing based on the optimized price and data you would receive when performing this experiment.

Why Enhanced RRBS With Us?
Service #S-1EMS
Bisulfite sequencing method utilized to detect nearly
all CpG sites across the entire genome at
base resolution detail
End-to-End Package:
Includes everything from sample preparation to data analysis
Bioinformatics:
FASTQ raw data, FASTQC quality control insights, read mapping, methylation calling and differential methylation analysis
Rapid Turnaround Time:
4 – 8 weeks from sample submission*
Sequencing & Library Preparation Guarantee:
Library QC by Bioanalyzer, KAPA quantification
and Q30 Sequencing score > 75%
Enhanced Double RRBS:
Higher CpG coverage than standard RRBS while being more cost-effective than WGBS (ideal for pilot studies)
High Sequencing Depth
Greater than 10X sequencing depth generating ~ 20 million reads per library
Post-Completion Technical Support:
Complimentary high-quality support to assist with data analysis at no additional charge

*Time measured when pre-payment for service is received

**Assuming 12 isolated DNA samples submitted and placed on 1 SR50 lane

What EpiGentek Provides

Example Data

Reduced Representation Bisulfite Sequencing Example Data & Figures
*These are examples of data that may be provided. Please consult your service representative regarding the exact data you will receive.


Sample Requirements

Epigenetic Services Sample Requirements

We currently accept DNA, cell, and tissue samples for our Reduced Representation Bisulfite Sequencing service. Proper sample preparation along with the appropriate quality control methods on your end will allow the greatest chance for assay success. Please follow all guidelines for sample requirements, packaging and labeling, and shipping which are outlined on the

Submission Instructions

page.

Talk to a Scientist

talk-to-a-scientist2

Want more information on our Reduced Representation Bisulfite Sequencing service or interested in customizing your research project? We’re here to help! Get in touch with one of our highly knowledgeable epigenetic services experts by calling toll-free at 1-877-374-4368 or emailing services @ epigentek.com.