RNA methylation ELISAs are a simple and convenient way to rapidly assess global levels of a specific methylated RNA modification, before pursuing more in-depth and costly applications such as qPCR and NGS. While analogous in terms of assay workflow (e.g., sample binding to assay wells, target capture by primary antibody, signal detection with enzyme-conjugated secondary antibody), compared with conventional ELISAs (enzyme-linked immunosorbent assay), RNA methylation ELISAs are extremely sensitive with much lower detection limits, allowing for the assessment of a wider range of sample types. Whether the target is 5mC or m6A, RNA methylation ELISAs provide a high-throughput, cost-effective means of analyzing a variety of modified RNA forms.
As pioneers in the R&D of epigenetics-based research tools, EpigenTek has leveraged its proprietary MethylFlash and EpiQuik technologies to develop rapid, accurate, and highly cited quantitation immunoassays for global RNA methylation analysis. These assays have been specially designed to use fully intact input RNA as starting material, eliminating the need for additional sample processing (e.g., denaturation, fragmentation) required by similar assays.