LoadingLoading
  • Browse By Category

  • Products
  • Services
  • Support
  • Corporate
  • Back
  • Products

  • Antibodies
  • Instruments
  • Research Kits
  • Proteins & Enzymes
  • Peptides
  • COVID-19 Assays
  • Back
  • Antibodies

  • Primary Antibodies
  • Secondary Antibodies
  • Tag Antibodies
  • Antibody Panel Packs
  • Back
  • Primary Antibodies

  • DNA Methylation Antibodies
  • Cell Structure & Function Antibodies
  • RNA Processing & Modification Antibodies
  • Chromatin & Transcription Antibodies
  • Other Primary Antibodies
  • Histone Modification Antibodies
  • Signal Transduction Antibodies
  • Metabolism Antibodies
  • Immunology & Inflammation Antibodies
  • Back
  • Cell Structure & Function Antibodies

  • DNA Damage & Repair Antibodies
  • Apoptosis & Cell Death Antibodies
  • Cell Cycle Antibodies
  • Cellular Structure Antibodies
  • Trafficking Antibodies
  • Back
  • Chromatin & Transcription Antibodies

  • DNA Replication & Transcription Antibodies
  • Chromatin Remodeling Antibodies
  • Cell Growth Antibodies
  • Development & Differentiation Antibodies
  • Back
  • Histone Modification Antibodies

  • Histone Methylation (Lysine) Antibodies
  • Histone Acetylation Antibodies
  • Histone Phosphorylation Antibodies
  • Ubiquitination & SUMOylation Antibodies
  • Histone Methylation (Arginine) Antibodies
  • Other Histone Methylation Antibodies
  • Other Histone Acetylation Antibodies
  • Histone Deacetylation Antibodies
  • Other Histone Antibodies
  • Back
  • Signal Transduction Antibodies

  • Cell Surface Receptor & Kinase Antibodies
  • Intracellular Kinase & Adaptor Protein Antibodies
  • Phosphatase Antibodies
  • Secreted Growth Factor Antibodies
  • Back
  • Metabolism Antibodies

  • Glucose Metabolism Antibodies
  • Lipid Metabolism Antibodies
  • Protein Metabolism Antibodies
  • Nucleotide Metabolism Antibodies
  • Back
  • Immunology & Inflammation Antibodies

  • Cell Marker & Receptor Antibodies
  • Cytokine Antibodies
  • Other Immunology Antibodies
  • Back
  • Antibody Panel Packs

  • Histone Modification
  • DNA Methylation
  • Back
  • Instruments

  • Sonication Devices
  • Magnets & Sample Mixers
  • Back
  • Research Kits

  • DNA Methylation
  • Chromatin & Transcription
  • Histone Methylation
  • Acetylation & Deacetylation
  • Gene Editing & Silencing
  • Sample Preparation
  • DNA Damage & Repair
  • Next Generation Sequencing
  • RNA Methylation
  • Other Histone Modifications
  • Back
  • DNA Methylation

  • DNA Bisulfite Conversion
  • DNA Methylation Quantification
  • DNA Methyltransferase & Demethylase Assays
  • Methylated DNA Immunoprecipitation
  • Methylated DNA Amplification
  • Back
  • Chromatin & Transcription

  • General Chromatin Immunoprecipitation
  • In Vitro Protein-DNA Interaction
  • Methyl-Histone Chromatin IP
  • Acetyl-Histone Chromatin IP
  • Methyl-DNA Binding Protein ChIP
  • Chromatin Analysis
  • Back
  • Histone Methylation

  • Histone Methyltransferase Assay
  • Histone Methylation Quantification
  • Histone Demethylase Assay
  • Back
  • Acetylation & Deacetylation

  • Histone Acetyltransferase (HAT) Assay
  • Histone Deacetylase (HDAC) Assay
  • Histone Acetylation Quantification
  • Back
  • Sample Preparation

  • DNA Preparation
  • Protein Preparation
  • Chromatin Preparation
  • RNA Preparation
  • Back
  • Other Histone Modifications

  • Sumoylation
  • Histone Phosphorylation Assay
  • Histone Citrullination
  • Back
  • Proteins & Enzymes

  • DNA Methylation/Demethylation Proteins
  • Methylated DNA Binding Proteins
  • Unmodified Histone Proteins
  • Chromatin Binding/Transcription Proteins
  • ADP Ribose Proteins
  • DNA Damage & Repair Proteins
  • Modified Histone Proteins
  • Back
  • DNA Methylation/Demethylation Proteins

  • DNA Demethylation Proteins
  • DNA Methylation Proteins
  • Back
  • Modified Histone Proteins

  • Histone Methylation Proteins
  • Histone Demethylation Proteins
  • Histone Acetylation Proteins
  • Histone Deacetylation Proteins
  • Histone Phosphorylation Proteins
  • Histone Sumoylation Proteins
  • Back
  • Peptides

  • Histone Methylation Peptides
  • Histone Acetylation Peptides
  • Histone Phosphorylation Peptides
  • Back
  • COVID-19 Assays

  • COVID-19 Kits
  • COVID-19 Antibodies
  • COVID-19 Proteins
  • Back
  • Services

  • DNA-based NGS Services
  • Chromatin-based NGS Services
  • qPCR-based Services
  • ELISA-based Services
  • Back
  • DNA-based NGS Services

  • Reduced Representation Bisulfite Sequencing (RRBS)
  • Whole Genome Bisulfite Sequencing (WGBS)
  • Targeted Bisulfite Sequencing
  • Back
  • Chromatin-based NGS Services

  • ChIP Sequencing (Histone Modifications)
  • Back
  • qPCR-based Services

  • Methylation-Specific qPCR (MSP)
  • Back
  • ELISA-based Services

  • Global DNA 5-mC Quantification
  • Global DNA 5-hmC Quantification
  • Global RNA 5-mC Quantification
  • Global RNA m6A Quantification
  • Histone Modification Quantification
  • Back
  • Support

  • Technical Support
  • Quality Assurance
  • Resources
  • Back
  • Technical Support

  • Submit Support Ticket
  • Back
  • Quality Assurance

  • Epigentek Gurantee
  • Risk-Free Testing: Pioneer Program
  • Product Review Program
  • Back
  • Resources

  • Epigenetics Newsletter
  • Intro to Epigenetics
  • What is Epigenetics?
  • Back
  • Corporate

  • Company Information
  • Procurement
  • Communication
  • Back
  • Company Information

  • About Epigentek
  • Press Release
  • Career Opportunities
  • Back
  • Procurement

  • Ordering Information
  • New Lab Startup Program
  • Distributors & Resellers
  • Back
  • Communication

  • Contact Us
  • Customer Feedback Survey
  • Events Calendar
Epigentek Home
Cart (0)
Track
Account
  • Products

    Antibodies

    • DNA Methylation
    • Histone Modification
      • Histone Methylation (Lysine)
      • Histone Methylation (Arginine)
      • Other Histone Methylation
      • Histone Acetylation
      • Histone Deacetylation
      • Other Histone Acetylation
      • Histone Phosphorylation
      • Ubiquitination & SUMOylation
      • Other Histone Antibodies
    • RNA Processing & Modification
    • Chromatin & Transcription
      • Chromatin Remodeling
      • DNA Replication & Transcription
      • Cell Growth
      • Development & Differentiation
    • Cell Structure & Function
      • Cell Cycle
      • DNA Damage & Repair
      • Apoptosis & Cell Death
      • Cellular Structure
      • Trafficking
    • Signal Transduction
      • Cell Surface Receptor & Kinase
      • Intracellular Kinase & Adaptor Protein
      • Phosphatase
      • Secreted Growth Factor
    • Metabolism
      • Glucose Metabolism
      • Lipid Metabolism
      • Protein Metabolism
      • Nucleotide Metabolism
    • Immunology & Inflammation
      • Cell Marker & Receptor
      • Cytokine
      • Other Immunology Antibodies
    • Other Primary Antibodies

    • Secondary Antibodies
    • Tag Antibodies
    • Antibody Panel Packs
      • Histone Modification Panel
      • DNA Methylation Panel

    Research Kits

    • Sample Preparation
      • DNA Preparation
      • RNA Preparation
      • Protein Preparation
      • Chromatin Preparation
    • Next Generation Sequencing
    • DNA Methylation
      • DNA Bisulfite Conversion
      • DNA Methylation Quantification
      • Methylated DNA Immunoprecipitation (meDIP)
      • DNMT & DNA Demethylase Assay
      • Methylated DNA Amplification
    • RNA Methylation
    • Chromatin & Transcription
      • Chromatin Immunoprecipitation
      • ChIP-seq
      • CUT&RUN
      • In Vitro Protein-DNA Interaction
      • Chromatin Accessibility
    • Histone Methylation
      • Histone Methylation Quantification
      • Histone Methytransferase Assay
      • Histone Demethylase Assay
    • Histone Acetylation
      • Histone Acetylation Quantification
      • Histone Acetylase (HAT) Assay
      • Histone Deacetylase (HDAC) Assay
    • Other Histone Modifications
      • Histone Citrullination
      • Histone Phosphorylation
      • SUMOylation
    • DNA Damage & Repair
    • Gene Editing & Silencing

    Proteins & Enzymes

    • DNA Methylation
      • DNA Methylation
      • DNA Demethylation
    • Modified Histones
      • Histone Methylation
      • Histone Demethylation
      • Histone Acetylation
      • Histone Deacetylation
      • Histone Phosphorylation
      • Histone SUMOylation
    • Unmodified Histones
    • Methylated DNA Binding
    • ADP Ribose
    • Chromatin Binding/Transcription
    • DNA Damage & Repair

    Peptides

    • Histone Methylation
    • Histone Acetylation/Deacetylation
    • Histone Phosphorylation

    Instruments

    • Sonication Devices
    • Magnets & Sample Mixers

    COVID-19 Assays

    • COVID-19 Kits
    • COVID-19 Antibodies
    • COVID-19 Proteins
  • Services

    DNA-based NGS Services

    • Reduced Representation Bisulfite Sequencing (RRBS)
    • Whole Genome Bisulfite Sequencing (WGBS)
    • Targeted Bisulfite Sequencing

    Chromatin-based NGS Services

    • ChIP Sequencing (Histone Modifications)

    qPCR-based Services

    • Methylation-Specific qPCR (MSP)

    ELISA-based Services

    • Global DNA 5-mC Quantification
    • Global DNA 5-hmC Quantification
    • Global RNA 5-mC Quantification
    • Global RNA m6A Quantification
    • Histone Modification Quantification
  • Support

    Technical Support

    • Submit Support Ticket
    • Download Datasheet

    Quality Assurance

    • EpigenTek Guarantee
    • Risk-Free Testing: Pioneer Program
    • Product Review Program

    Resources

    • Epigenetics Newsletter
    • Intro to Epigenetics
    • What Is Epigenetics?
  • Corporate

    Company Information

    • About EpigenTek
    • Corporate Sustainability
    • Press Releases
    • Career Opportunities

    Procurement

    • Ordering Information
    • New Lab Startup Program
    • Distributors & Resellers

    Communication

    • Contact Us
    • Customer Feedback Survey
    • Events Calendar
 
EpiGentek Guarantee
   Home  »  Research Kits  »  DNA Methylation  »  DNA Bisulfite Conversion 
  Quote Lookup

DNA Bisulfite Conversion

bisulfite conversion and PCR amplification of methylated DNABisulfite conversion is a technique in which cytosine is converted to uracil using sodium bisulfite, while 5-methylcytosine (5-mC) remains intact. Bisulfite and sodium bisulfite-based methods are common approaches used to study DNA methylation and help in preparing genomic DNA for gene-specific DNA methylation analysis. Bisulfite conversion is often followed by downstream applications such as PCR or sequencing.

Unmethylated cytosine residues are deaminated to uracil and methylated cytosine (5-mC) residues remain unaffected, enabling PCR amplification to recognize uracils as thymines and 5-mC or 5-hmC as cytosines. This allows researchers to differentiate between methylated and unmethylated cytosine residues, offering single-nucleotide resolution information about the methylated areas of DNA. Complete conversion is necessary for a successful DNA methylation study, along with reducing the amount of DNA degradation that will occur as a result of the typically harsh chemical reaction.

chemical structure showing bisulfite conversion of cytosine to uracilPopular downstream methods for analyzing DNA methylation on a gene-specific basis following bisulfite treatment include bisulfite sequencing, methylation specific PCR (MS-PCR), and methylation-based microarrays. Considering your downstream application can help in determining which bisulfite conversion method is ideal, especially due to the variation in post-conversion fragment size. The BisulFlash DNA Modification Kit is geared specifically toward real-time MSP whereas the best-selling Methylamp DNA Modification Kit is designed to be compatible with Illumina workflows and next-generation sequencing (NGS). If a quicker and easier bisulfite conversion alternative is desired prior to sequencing, the BisulFlash DNA Bisulfite Conversion Easy Kit can be completed in just 60 minutes and uses a liquid bisulfite conversion reagent for convenience.

Beginning with high quality DNA is critical, especially because the acidic nature of the conversion process will fragment the DNA. For samples containing low amounts of DNA, the Methylamp Whole Cell Bisulfite Modification Kit is recommended, as it can directly convert from cells, tissues, blood, and other starting materials. When handling large scale bisulfite conversion experiments, high-throughput options are critical in order to save time and reduce cost. In this case, the BisulFlash DNA Bisulfite Conversion Mag-96 Kit is specifically streamlined for high-throughput and uses a magnetic bead-based 96-well format. For a detailed comparison of bisulfite conversion kits, see the table below.

 

Methylamp DNA Modification KitCat #P-1001

BisulFlash DNA Modification KitCat #P-1026

Methylamp Whole Cell Bisulfite Modification KitCat #P-1016

  More DetailsMore DetailsMore Details
Codename"Classic""Fast""Direct"
PurposeChemical-based DNA bisulfite conversionFast heat-based DNA bisulfite conversionDirectly convert from cells, tissues, saliva, urine, or blood
Key Points
  • Illumina workflow compatible
  • Most robust and reliable
  • Ideal for bisulfite sequencing, NGS, and various MSP
  • Extremely fast
  • Ideal for real-time MSP
  • Optional sequencing protocol
  • Eliminates separate DNA isolation step
  • Ideal for samples containing low quantities of DNA
Starting MaterialDNADNAcells, tissue, blood
Lowest Input Amount50 pg200 pg100 cells, 1 µl blood
Total Protocol Time< 2 hours30 minutes< 3 hours
Elution Volume8-20 µl10-20 µl8-20 µl
Conversion Efficiency99.9%99.9%99.9%
Desulfonation/Clean-Up
Recovery>75%>75%>85%
Post-Conversion DNA Fragment Size200-2000 bp; Avg. Peak of 800 bp100-400 bp; Avg. Peak of 250 bp200-2000 bp; Avg. Peak of 800 bp
Vessel FormatSpin ColumnSpin ColumnSpin Column
 
Result Pages:  1  Displaying 1 to 12 (of 12 products)
Image Description Cat. No. Size Price
Methylamp DNA Modification Kit
Methylamp DNA Modification Kit

Citations (211)
Input Type: DNA
Research Area: DNA Methylation
Target Application: Sample Modification
Vessel Format: Columns/Tubes
P-1001-140 reactions $136.00 Add to Cart
P-1001-280 reactions $236.00 Add to Cart
Research Kits
Methylamp Coupled DNA Isolation & Modification Kit
Methylamp Coupled DNA Isolation & Modification Kit

Citations (2)
Input Type: DNA
Research Area: DNA Methylation
Target Application: Sample Isolation, Sample Modification
Vessel Format: Columns/Tubes
P-1002-4040 samples $238.00 Discontinued
Research Kits
Methylamp 96 DNA Modification Kit
Methylamp 96 DNA Modification Kit

Citations (1)
Input Type: DNA
Research Area: DNA Methylation
Target Application: Sample Modification
Vessel Format: 96-Well Plate
P-1008-196 samples $230.00 Add to Cart
P-1008-2192 samples $370.00 Discontinued
Research Kits
Methylamp One-Step DNA Modification Kit
Methylamp One-Step DNA Modification Kit

Citations (25)
Input Type: DNA
Research Area: DNA Methylation
Target Application: Sample Modification
Vessel Format: Columns/Tubes
P-1010-140 samples $135.00 Add to Cart
P-1010-280 samples $230.00 Add to Cart
Research Kits
Methylamp Universal Methylated DNA Kit
Methylamp Universal Methylated DNA Kit

Citations (8)
Input Type: DNA
Research Area: DNA Methylation
Target Application: Sample Modification
Vessel Format: Columns/Tubes
P-1011-110 modifications $266.00 Add to Cart
P-1011-220 modifications $402.00 Add to Cart
Research Kits
Methylamp Whole Cell Bisulfite Modification Kit
Methylamp Whole Cell Bisulfite Modification Kit

Citations (7)
Input Type: DNA
Research Area: DNA Methylation
Target Application: Sample Modification
Vessel Format: Columns/Tubes
P-1016-4040 samples $194.00 Add to Cart
P-1016-8080 samples $346.00 Add to Cart
Research Kits
BisulFlash DNA Modification Kit
BisulFlash DNA Modification Kit

Citations (132) | (2)
Input Type: DNA
Research Area: DNA Methylation
Target Application: Sample Modification
Vessel Format: Columns/Tubes
P-1026-05050 reactions $148.00 Add to Cart
Research Kits
BisulFlash DNA Bisulfite Conversion Mag-96 Kit
BisulFlash DNA Bisulfite Conversion Mag-96 Kit

Input Type: DNA
Research Area: DNA Methylation
Target Application: Sample Modification
Vessel Format: 96-Well Plate
P-1050-09696 reactions $195.00 Discontinued
P-1050-192192 reactions $359.00 Discontinued
Research Kits
BisulFlash DNA Bisulfite Conversion Easy Kit
BisulFlash DNA Bisulfite Conversion Easy Kit

Citations (3)
Input Type: DNA
Research Area: DNA Methylation
Target Application: Sample Modification
Vessel Format: Columns/Tubes
P-1054-05050 reactions $140.00 Discontinued
Research Kits
EpiNext High-Sensitivity Bisulfite-Seq Kit (Illumina)
EpiNext High-Sensitivity Bisulfite-Seq Kit (Illumina)

Citations (6) | (1)
Input Type: DNA
Research Area: DNA Methylation, Next Gen Sequencing
Target Application: Library Construction, Sample Modification
Vessel Format: Columns/Tubes
P-1056A-1212 reactions $624.00 Add to Cart
P-1056A-2424 reactions $1,114.00 Add to Cart
Research Kits
BisulPlus Loci 5mC & 5hmC Detection PCR Kit
BisulPlus Loci 5mC & 5hmC Detection PCR Kit

Citations (1)
Input Type: DNA
Research Area: DNA Methylation
Target Application: Sample Modification
Vessel Format: Columns/Tubes
P-1067-482x 24 reactions $525.00 Add to Cart
Research Kits
EpiNext RRBS Library Fast Kit

Input Type: DNA
Research Area: DNA Methylation, Next Gen Sequencing
Target Application: Library Construction, Sample Modification
Vessel Format: Columns/Tubes
P-1069-1212 reactions $655.00 $622.25 Add to Cart
P-1069-2424 reactions $1,123.00 $1,066.85 Add to Cart
Research Kits
Result Pages:  1  Displaying 1 to 12 (of 12 products)
Refine Your Results
(9)
(9)
(2)
(9)
(2)
(0)
(8)
(1)
BOGO-Conjugate Abs
Featured Areas of Study
DNA, Protein, & Chromatin Preparation
DNA Methylation & Demethylation Analysis
Chromatin Immunoprecipitation
Histone Methylation & Demethylation
Histone Acetylation & Deacetylation
Gene Expression & Silencing
DNA Damage & Repair
Histone Phosphorylation
SUMOylation
Next-Generation Sequencing
Products & Services
Ordering Information & Return Policy
Epigenetic Services
Discounts & Promotions
Rewards Program
Product Reviews
New Lab Startup Program
Risk-Free Testing: Pioneer Program

Support
Download Datasheets
Epigentek Guarantee
Technical Support
Corporate
About Epigentek
Press Releases
Product Newsletter
Events Calendar
Distributors & Resellers
EpigenCare
Career Opportunities
Contact Us

Stay Informed



Terms & Conditions | Privacy Policy | Site Map
Copyright © EpiGentek Group Inc. All rights reserved.
  • Stay Informed:
Terms & Conditions | Privacy Policy Copyright © 2020 EpiGentek
➤