The Methylamp™ Coupled DNA Modification Kit is a complete set of essential components which enables the experimenter to isolate and modify DNA efficiently.The entire procedure can be completed within 2 hours and 20 minutes and produces far superior results than any competitor kits. The Methylamp™ Coupled DNA Modification Kit is suitable for MS-PCR, real time MS-PCR, methylation sequencing, and pyrosequencing, as well as methylation microarray. The kit has the following advantages:
Principle & ProcedureThe Methylamp™ Coupled DNA Isolation and Modification Kit contains all reagents required for DNA isolation and bisulfite conversion. DNA is isolated and chemically denatured to allow bisulfite reagent to react specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. The unique DNA protection reagents contained in the modification buffer can prevent the chemical and thermophilic degradation of DNA in the bisulfite treatment. The non-toxic modified DNA capture buffer enables DNA to tightly bind to the column filter, thus DNA cleaning can be carried out on the column to effectively remove residual sodium bisulfite and salts. Modified DNA then can be eluted and stably stored at -20°C for up to 2 months.
Fig. 1. Schematic procedure for using the Methylamp Coupled DNA Isolation & Modification Kit (Colorimetric).
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