Sitewide 2017 Summer Sale Site-wide summer sale! Take 10% off your entire order when using code E1707SUN. » Learn More
Epigentek Home
 
Epigentek Free Shipping

Methylamp Coupled DNA Isolation & Modification Kit

Share:

Citations (2) | Write a Review
Schematic procedure for using the Methylamp Coupled DNA Isolation & Modification Kit (Colorimetric).
Input Type: DNA
Research Area: DNA Methylation
Target Application: Sample Isolation, Sample Modification
Vessel Format: Columns/Tubes
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-1002-4040 samples $225.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

The Methylamp™ Coupled DNA Modification Kit is a complete set of essential components which enables the experimenter to isolate and modify DNA efficiently.The entire procedure can be completed within 2 hours and 20 minutes and produces far superior results than any competitor kits. The Methylamp™ Coupled DNA Modification Kit is suitable for MS-PCR, real time MS-PCR, methylation sequencing, and pyrosequencing, as well as methylation microarray. The kit has the following advantages:

  • The fastest procedure available, which can be finished within 2 hours and 20 minutes with consistent reaction conditions.
  • High efficiency of DNA isolation from multiple resources of biological material containing tiny amounts of DNA (as low as 0.1 ng), especially from serum, plasma and body fluids.
  • Completely converts unmethylated cytosine into uracil: modified DNA > 99.98%.
  • The lowest degradation of DNA in the modification process: more than 90% of DNA loss can be prevented.
  • The lowest requirement of starting DNA for modification: only 50 pg or 20 cells. Simple, reliable, and consistent modification conditions.

Principle & Procedure
The Methylamp™ Coupled DNA Isolation and Modification Kit contains all reagents required for DNA isolation and bisulfite conversion. DNA is isolated and chemically denatured to allow bisulfite reagent to react specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. The unique DNA protection reagents contained in the modification buffer can prevent the chemical and thermophilic degradation of DNA in the bisulfite treatment. The non-toxic modified DNA capture buffer enables DNA to tightly bind to the column filter, thus DNA cleaning can be carried out on the column to effectively remove residual sodium bisulfite and salts. Modified DNA then can be eluted and stably stored at -20°C for up to 2 months.

 
Fig. 1. Schematic procedure for using the Methylamp Coupled DNA Isolation & Modification Kit (Colorimetric).

Product Components

MR1 (DNA Digestion Solution)
MR2 (DNA Digestion Powder)
MR3 (DNA Isolation Buffer)
MR4 (DNA Isolation Enhancer)
MR5 (DNA Denature Solution)
MR6 (DNA Modifier Powder)
MR7 (DNA Modifier Solution)
MR8 (DNA Binding Buffer)
MR9 (DNA Washing Buffer)
MR10 (DNA Elution buffer)
1X TE Buffer
F-Spin Column
F-Collection Tube
User Guide

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

Epigentek Product Reviews

Researchers Also Purchased
MethylFlash Methylated DNA 5-mC Quantification Kit (Colorimetric)
MethylFlash Methylated DNA 5-mC Quantification Kit (Colorimetric)
EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit
EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit
Methylamp DNA Modification Kit
Methylamp DNA Modification Kit
EpiQuik Nuclear Extraction Kit II (Nucleic Acid-Free)
EpiQuik Nuclear Extraction Kit II (Nucleic Acid-Free)
Epigentek is an epigenetics company that specializes in epigenetic kits, antibodies, reagents, and services for epigenetic research in DNA methylation, histone modification, and chromatin studies. Terms & Conditions | Privacy Policy | Site Map
Copyright © 2017 Epigentek Group Inc. All rights reserved.