Histone extraction is a critical step in epigenetics research, particularly in the study of histone post-translational modifications (PTMs). Histones are small, positively charged proteins around which DNA is wrapped in the cell nucleus. They play a fundamental role in gene regulation and chromatin structure. For accurate quantification and analysis of histone PTMs, researchers need to isolate histone proteins from cellular or tissue samples, an essential process for separating histones from other cellular components such as DNA, RNA, and non-histone proteins.
High-quality histone extracts are required for various downstream assays, among which include ELISA-based histone PTM quantification, mass spectrometry analysis, and western blotting. These assays provide insights into the epigenetic regulation of gene expression, which is pivotal in understanding various biological processes like development, disease, and cellular differentiation. EpigenTek’s total histone extraction kits offer a simple, selective, and rapid means of efficiently extracting histone proteins while preserving their modifications, including acetylation, methylation, phosphorylation, ubiquitination, and sumoylation.
For more information on histone preparation techniques and our protein preparation products and services, please contact our support team.