Epigentek Home
 
EpiGentek Guarantee
      »    »      Quote Lookup
Refine Your Results
Save on CRISPR Cas9 (SaCas9)
DNA Methylation

DNA Methylation

Figure of methyl group added to DNA known as DNA methylation, an epigenetic mechanismDNA methylation is a major epigenetic modification involving the addition of a methyl group to the 5 position of cytosine by DNA methyltransferase to form 5-methylcytosine (5-mC). This epigenetic mark has the power to turn genes on or off and can be inherited through cell division. DNA methylation was the first epigenetic mark to be discovered and it plays an important role in normal human development, aging, tumorigenesis, and other genetic and epigenetic diseases. Similarly, DNA hydroxymethylation, caused by oxidation of 5-mC through the TET family of enzymes, was further discovered to be involved in controlling gene expression and is a key DNA demethylation process as part of the DNA methylation cycle.

Techniques to measure 5-mC have developed over the years and can vary from heat-based to chemical-based methods. Bisulfite conversion is a popular technique used for preparing DNA for methylation analysis on a gene-specific level. This process converts cytosine to uracil while leaving 5-methylcytosine intact to allow methylation analysis at single-nucleotide resolution. Not only is efficient conversion necessary, but recovery must be optimal due to the harsh chemical reaction which often degrades the DNA.

Obtaining an overall view of a sample’s methylation status is a smart way to pre-screen your samples before pursuing downstream applications. Our MethylFlash global DNA methylation pre-screening tools can be used prior to more in depth investigation and costly sequencing. Conventional methods such as mass spectrometry or chromatography, which require expensive equipment or specialized expertise, are not necessary for this ELISA-based method. Also, unlike LUMA or LINE-1, Alu, and LTR-based assays, MethylFlash technology directly quantifies actual global DNA methylation. Methylated cytosine chemical structure from cytosine to 5-methylcytosine, 5-mC

Additional methods for investigating DNA methylation include an antibody-based technique known as methylated DNA immunoprecipitation (MeDIP) that is used for studying gene-specific DNA methylation on a genome-wide scale, activity/inhibition assays that measure DNA methyltransferase (DNMT) and other enzymes related to the process of DNA methylation, as well as an amplification technique involving methylation-specific PCR (MS-qPCR) that is used for studying gene or sequence-specific DNA methylation.

DNA Methylation Tools

DNA Bisulfite Conversion
DNA Methylation Quantification
Methylated DNA Immunoprecipitation (MeDIP)
DNA Methyltransferase & Demethylase Assays
Methylated DNA Amplification (MS-qPCR)
Gene-specific, base-resolution DNA methylation Global DNA methylation Gene-specific DNA methylation on a genome wide scale Activity/inhibition of enzymes involved in DNA methylation cycle Gene or sequence-specific DNA methylation
 
DNA Bisulfite Conversion
Bisulfite conversion is a popular technique used to study DNA methylation that involves converting cytosine to uracil while leaving 5-methylcytosine (5-mC) intact. Bisulfite and sodium bisulfite based methods are common approaches for preparing genomic DNA ...

DNA Methylation Quantification
DNA methylation plays an important role in normal organismal development and in cellular differentiation in higher organisms. Gene expression, as well as the development of nearly all types of cancer, are also tied to DNA methylation. For example, global decrease in 5-methylcytosine content (DNA hypomethylation) is likely caused by methyl-deficiency due to a variety of environmental influences, and has been proposed as a molecular marker in multiple biological processes such as cancer. Global quantification of DNA methylation is crucial for understanding the roles that gene expression and silencing play in the development of cancer and other diseases.

Methylated DNA Immunoprecipitation
Methylated DNA immunoprecipitation (meDIP) is a large scale antibody-based technique that is used to enrich and capture methylated DNA fragments for use in gene-specific DNA methylation studies on a genome wide scale. Following meDIP approaches, DNA methylation can be analyzed using a variety of downstream applications including meDIP-PCR, meDIP-ChIP, and meDIP-sequencing.

DNA Methyltransferase & Demethylase Assays
DNA methyltransferases, or DNMTs, catalyze DNA methylation by adding methyl groups to the 5-carbon position of the cytosine ring, resulting in 5-methylcytosine. The various types of DNMTs are responsible for the maintenance and establishment of DNA methylation patterns. The ten-eleven translocation, or TET, family of 5-mC hydroxylase enzymes including TET1 are responsible for oxidizing 5-methylcytosine into 5-hydroxymethylcytosine. Measuring DNMT and TET amounts, activity, and inhibition levels will allow us to better understand the relationships between cytosine, 5-methylcytosine, and 5-hydroxymethylcytosine as part of the DNA methylation and DNA demethylation cycle.

Methylated DNA Amplification
Methylation specific PCR (MSP or MS-PCR) and bisulfite sequencing are some of the most commonly used methods for analyzing gene or sequence-specific DNA methylation data, usually after bisulfite treatment of DNA. After conversion, selective methylation amplification of a target region of methylated sequences with methylation primers is often necessary in methylation analysis protocols.

Result Pages:  1  2  3  [Next >>]  Displaying 1 to 20 (of 44 products)
Image Description Cat. No. Size Price
Methylamp DNA Modification Kit
Methylamp DNA Modification Kit

Citations (145)
Input Type: DNA
Research Area: DNA Methylation
Target Application: Sample Modification
Vessel Format: Columns/Tubes
P-1001-140 reactions $111.00 Add to Cart
P-1001-280 reactions $199.00 Add to Cart
Research Kits
Methylamp Coupled DNA Isolation & Modification Kit
Methylamp Coupled DNA Isolation & Modification Kit

Citations (2)
Input Type: DNA
Research Area: DNA Methylation
Target Application: Sample Isolation, Sample Modification
Vessel Format: Columns/Tubes
P-1002-4040 samples $225.00 Add to Cart
Research Kits
Methylamp 96 DNA Modification Kit
Methylamp 96 DNA Modification Kit

Citations (1)
Input Type: DNA
Research Area: DNA Methylation
Target Application: Sample Modification
Vessel Format: 96-Well Plate
P-1008-196 samples $199.00 Add to Cart
P-1008-2192 samples $370.00 Discontinued
Research Kits
Methylamp One-Step DNA Modification Kit
Methylamp One-Step DNA Modification Kit

Citations (20)
Input Type: DNA
Research Area: DNA Methylation
Target Application: Sample Modification
Vessel Format: Columns/Tubes
P-1010-140 samples $112.00 Add to Cart
P-1010-280 samples $199.00 Add to Cart
Research Kits
Methylamp Universal Methylated DNA Kit
Methylamp Universal Methylated DNA Kit

Citations (4)
Input Type: DNA
Research Area: DNA Methylation
Target Application: Sample Modification
Vessel Format: Columns/Tubes
P-1011-110 modifications $225.00 Add to Cart
P-1011-220 modifications $338.00 Add to Cart
Research Kits
Methylamp Methylated DNA Capture (MeDIP) Kit
Methylamp Methylated DNA Capture (MeDIP) Kit

Citations (26)
Input Type: DNA
Research Area: DNA Methylation
Target Application: Immunoprecipitation
Vessel Format: 96-Well Plate
P-1015-2424 reactions $317.00 Add to Cart
P-1015-4848 reactions $534.00 Add to Cart
Research Kits
Methylamp Whole Cell Bisulfite Modification Kit
Methylamp Whole Cell Bisulfite Modification Kit

Citations (5)
Input Type: DNA
Research Area: DNA Methylation
Target Application: Sample Modification
Vessel Format: Columns/Tubes
P-1016-4040 samples $163.00 Add to Cart
P-1016-8080 samples $299.00 Add to Cart
Research Kits
DNA Methylation/Hydroxymethylation Standard Set
DNA Methylation/Hydroxymethylation Standard Set

Input Type: DNA
Research Area: DNA Methylation
Target Application: Assay Validation
Vessel Format: Columns/Tubes
P-1025-12 µg/standard x 3 $338.00 Add to Cart
Research Kits
BisulFlash DNA Modification Kit
BisulFlash DNA Modification Kit

Citations (60) | (2)
Input Type: DNA
Research Area: DNA Methylation
Target Application: Sample Modification
Vessel Format: Columns/Tubes
P-1026-05050 reactions $122.00 Add to Cart
Research Kits
Methylamp MS-qPCR Fast Kit
Methylamp MS-qPCR Fast Kit

Citations (1)
Input Type: DNA
Research Area: DNA Methylation
Target Application: PCR Amplification
Vessel Format: 96-Well Plate, Columns/Tubes
P-1028-100100 reactions $163.00 Add to Cart
P-1028-200200 reactions $297.00 Add to Cart
Research Kits
EpiQuik Quantitative PCR Fast Kit
EpiQuik Quantitative PCR Fast Kit

Citations (1)
Input Type: DNA
Research Area: DNA Methylation
Target Application: PCR Amplification
Vessel Format: 96-Well Plate, Columns/Tubes
P-1029-100100 reactions $163.00 Add to Cart
P-1029-200200 reactions $299.00 Add to Cart
Research Kits
MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric)
MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric)

Citations (5) | (2)
Input Type: DNA
Research Area: DNA Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
P-1030-4848 reactions $299.00 Add to Cart
P-1030-9696 reactions $539.00 Add to Cart
Research Kits
MethylFlash Global DNA Hydroxymethylation (5-hmC) ELISA Easy Kit (Colorimetric)
MethylFlash Global DNA Hydroxymethylation (5-hmC) ELISA Easy Kit (Colorimetric)

Citations (1) | (1)
Input Type: DNA
Research Area: DNA Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
P-1032-4848 reactions $318.00 Add to Cart
P-1032-9696 reactions $559.00 Add to Cart
Research Kits
MethylFlash Methylated DNA 5-mC Quantification Kit (Colorimetric)
MethylFlash Methylated DNA 5-mC Quantification Kit (Colorimetric)

Citations (273) | (4)
Input Type: DNA
Research Area: DNA Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
P-1034-4848 assays $299.00 Add to Cart
P-1034-9696 assays $539.00 Add to Cart
Research Kits
MethylFlash Methylated DNA Quantification Kit (Fluorometric)
MethylFlash Methylated DNA Quantification Kit (Fluorometric)

Citations (24) | (1)
Input Type: DNA
Research Area: DNA Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
P-1035-4848 assays $317.00 Add to Cart
P-1035-9696 assays $559.00 Add to Cart
Research Kits
MethylFlash Hydroxymethylated DNA 5-hmC Quantification Kit (Colorimetric)
MethylFlash Hydroxymethylated DNA 5-hmC Quantification Kit (Colorimetric)

Citations (57)
Input Type: DNA
Research Area: DNA Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
P-1036-4848 assays $318.00 Add to Cart
P-1036-9696 assays $559.00 Add to Cart
Research Kits
MethylFlash Hydroxymethylated DNA Quantification Kit (Fluorometric)
MethylFlash Hydroxymethylated DNA Quantification Kit (Fluorometric)

Citations (8)
Input Type: DNA
Research Area: DNA Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
P-1037-4848 assays $348.00 Add to Cart
P-1037-9696 assays $599.00 Add to Cart
Research Kits
EpiQuik Hydroxymethylated DNA Immunoprecipitation (hMeDIP) Kit
EpiQuik Hydroxymethylated DNA Immunoprecipitation (hMeDIP) Kit

Citations (6) | (1)
Input Type: DNA
Research Area: DNA Methylation
Target Application: Immunoprecipitation
Vessel Format: 96-Well Plate
P-1038-2424 reactions $338.00 Add to Cart
P-1038-4848 reactions $599.00 Add to Cart
P-1038-9696 reactions $1,059.00 Add to Cart
Research Kits
MethylFlash Urine 5-Methylcytosine (5-mC) Quantification Kit  (Colorimetric)
MethylFlash Urine 5-Methylcytosine (5-mC) Quantification Kit (Colorimetric)

Input Type: Urine
Research Area: DNA Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
P-1039-4848 assays $277.00 Add to Cart
P-1039-9696 assays $493.00 Add to Cart
Research Kits
MethylFlash Urine 5-Methylcytosine (5-mC) Quantification Kit  (Fluorometric)
MethylFlash Urine 5-Methylcytosine (5-mC) Quantification Kit (Fluorometric)

Input Type: Urine
Research Area: DNA Methylation
Target Application: Amount Quantitation
Vessel Format: 96-Well Plate
P-1040-4848 assays $277.00 Add to Cart
P-1040-9696 assays $493.00 Add to Cart
Research Kits
Result Pages:  1  2  3  [Next >>]  Displaying 1 to 20 (of 44 products)
Epigentek is an epigenetics company that specializes in epigenetic kits, antibodies, reagents, and services for epigenetic research in DNA methylation, histone modification, and chromatin studies. Terms & Conditions | Privacy Policy | Site Map
Copyright © 2017 Epigentek Group Inc. All rights reserved.