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DNA Methylation

DNA methylation is an epigenetic modification where a methyl group is added to cytosine bases, typically at CpG sites, by DNA methyltransferases (DNMTs), influencing gene expression without altering the DNA sequence. This modification can be dynamically regulated, as ten-eleven translocation (TET) enzymes catalyze the oxidation of 5-methylcytosine (5-mC) into hydroxymethylcytosine (5-hmC) and further into formylcytosine (5-fC) and carboxylcytosine (5-caC), facilitating active DNA demethylation.

Chemical pathway illustrating cytosine methylation by DNMT and demethylation by TET enzymes, showing 5mC, 5hmC, 5fC, and 5caC.

Chemical pathway illustrating cytosine methylation by DNMT and demethylation by TET enzymes, showing 5mC, 5hmC, 5fC, and 5caC.

Global DNA Modification Quantification Assays

Bar chart comparing 5-mC levels

Quantify levels of 5-mC, 5-hmC, 5-fC, or 8-OHdG in total DNA

Enzymatic Assays

Representative data chart

Measure activity and inhibition of DNA methylases and demethylases

DNA Bisulfite Assays

Sanger sequencing chromatograms

Differentiate between methylated and unmethylated cytosines gene specifically or genome wide

Methylated DNA Immunoprecipitation Assays

Schematic workflow diagram

Enrich for 5-mC or 5-hmC DNA sequences

DNA Methylation Tools

DNA Bisulfite Conversion

Global DNA Methylation Quantification

DNA methylation is an important epigenetic modification that plays a key role in the regulation of gene expression. It involves the addition of a methyl group to cytosine bases in the DNA molecule and can occur at specific sites in the genome known as CpG islands. Methylation can affect the way that genes are expressed by either activating or silencing them, depending on the specific context and locat...

Methylated DNA Immunoprecipitation

Introduction Methylated DNA immunoprecipitation (MeDIP) is a technique used to identify regions of the genome that are methylated, and involves the specific capture and enrichment of methylated DNA fragments. Methylation is an epigenetic modification that can affect gene expression and is involved in a variety of biological processes, including development, differentiation, and genomic imprinting. Method The DNA is fragmented An antibody that specifically recognizes methylated cytosine residues in DNA is used The DNA is incubated with the antibody The antibody binds to the methylated DNA, forming a DNA-antibody complex The DNA-antibody complex is isolated using a process called immunoprecipitation The isolated DNA is analyzed using techniques such as polymerase chain reaction (PCR) or sequencing to identify the specific regions of the genome that are methylated Applications MeDIP is often used in conjunction with other techniques, such as microarray analysis or next-generation sequencing, to study the role of DNA methylation in various biological processes and to identify potential therapeutic targets for diseases related to abnormal DNA methylation patterns. Methylamp Methylated DNA Capture (MeDIP) KitCat #P-1015EpiQuik Hydroxymethylated DNA Immunoprecipitation (hMeDIP) KitCat #P-1038EpiQuik MeDIP Ultra KitCat #P-1052EpiQuik Methylated DNA Immunoprecipitation KitCat #P-2019EpiQuik Tissue Methylated DNA Immunoprecipitation KitCat #P-2020 More Details More Details More Details More Details More Details Purpose Enrich and capture methylated DNA fragments Enrich and capture hydroxymethylated DNA fragments Enrich and capture methylated DNA fragments from low starting input Enrich and capture methylated DNA fragments directly from cell lysates Enrich and capture methylated DNA fragments directly from tissues Key Points Ideal for PCR and microarray Illumina workflow compatible Compatible with downstream workflows including hMeDIP-PCR and hMeDIP-chip Ideal for MeDIP-Seq Illumina workflow compatible Most sensitive and specific Eliminates DNA

DNA Methyltransferase & Demethylase Assays

DNA methyltransferases, or DNMTs, catalyze DNA methylation by adding methyl groups to the 5-carbon position of the cytosine ring, resulting in 5-methylcytosine. The various types of DNMTs are responsible for the maintenance and establishment of DNA methylation patterns. The ten-eleven translocation, or TET, family of 5-mC hydroxylase enzymes including TET1 are responsible for oxidizing 5-methylcytosine into 5-hydroxymethylcytosine. Measuring DNMT and TET amounts, activity, and inhibition levels will allow us to better understand the relationships between cytosine, 5-methylcytosine, and 5-hydroxymethylcytosine as part of the DNA methylation and DNA demethylation cycle. This in turn can help us start to better understand and use these findings to find and fight epigenetic diseases.

Methylated DNA Amplification

Methylation-specific PCR (MSP or MS-PCR) and bisulfite sequencing are techniques used to study the methylation status of cytosine bases in DNA. DNA methylation is a chemical modification that involves the addition of a methyl group to the cytosine base of a cytosine-phosphate-guanine (CpG) dinucleotide. DNA methylation is an important epigenetic mechanism that plays a role in the regulation of gene expression, and changes in DNA methylation pattern...

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Displaying 1 to 20 (of 49 products)

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Methylamp DNA Modification Kit

Methylamp DNA Modification Kit

Citations (221)

Input Type:	DNA
Research Area:	DNA Methylation
Target Application:	Sample Modification
Vessel Format:	Columns/Tubes

P-1001-1	40 reactions	$140.00
P-1001-2	80 reactions	$243.00

Research Kits

Methylamp Coupled DNA Isolation & Modification Kit

Methylamp Coupled DNA Isolation & Modification Kit

Citations (2)

Input Type:	DNA
Research Area:	DNA Methylation
Target Application:	Sample Isolation, Sample Modification
Vessel Format:	Columns/Tubes

P-1002-40

40 samples

$238.00

Discontinued

Research Kits

Methylamp 96 DNA Modification Kit

Methylamp 96 DNA Modification Kit

Citations (2)

Input Type:	DNA
Research Area:	DNA Methylation
Target Application:	Sample Modification
Vessel Format:	96-Well Plate

P-1008-1	96 samples	$237.00
P-1008-2	192 samples	$370.00	Discontinued

Research Kits

Methylamp One-Step DNA Modification Kit

Methylamp One-Step DNA Modification Kit

Citations (26)

Input Type:	DNA
Research Area:	DNA Methylation
Target Application:	Sample Modification
Vessel Format:	Columns/Tubes

P-1010-1	40 samples	$139.00
P-1010-2	80 samples	$237.00

Research Kits

Methylamp Universal Methylated DNA Kit

Methylamp Universal Methylated DNA Kit

Citations (8)

Input Type:	DNA
Research Area:	DNA Methylation
Target Application:	Sample Modification
Vessel Format:	Columns/Tubes

P-1011-1	10 modifications	$279.00
P-1011-2	20 modifications	$423.00

Research Kits

Methylamp Methylated DNA Capture (MeDIP) Kit

Methylamp Methylated DNA Capture (MeDIP) Kit

Citations (56)

Input Type:	DNA
Research Area:	DNA Methylation
Target Application:	Immunoprecipitation
Vessel Format:	96-Well Plate

P-1015-24	24 reactions	$398.00
P-1015-48	48 reactions	$667.00

Research Kits

Methylamp Whole Cell Bisulfite Modification Kit

Methylamp Whole Cell Bisulfite Modification Kit

Citations (7)

Input Type:	DNA
Research Area:	DNA Methylation
Target Application:	Sample Modification
Vessel Format:	Columns/Tubes

P-1016-40	40 samples	$199.00
P-1016-80	80 samples	$356.00

Research Kits

DNA Methylation/Hydroxymethylation Standard Set

DNA Methylation/Hydroxymethylation Standard Set

Citations (1)

Input Type:	DNA
Research Area:	DNA Methylation
Target Application:	Assay Validation
Vessel Format:	Columns/Tubes

P-1025-1

2 µg/standard x 3

$359.00

Discontinued

Research Kits

BisulFlash DNA Modification Kit

BisulFlash DNA Modification Kit

Citations (139) | (3)

Input Type:	DNA
Research Area:	DNA Methylation
Target Application:	Sample Modification
Vessel Format:	Columns/Tubes

P-1026-050

50 reactions

$152.00

Research Kits

Methylamp MS-qPCR Fast Kit

Methylamp MS-qPCR Fast Kit

Citations (14)

Input Type:	DNA
Research Area:	DNA Methylation
Target Application:	PCR Amplification
Vessel Format:	96-Well Plate, Columns/Tubes

P-1028-100	100 reactions	$204.00
P-1028-200	200 reactions	$364.00

Research Kits

EpiQuik Quantitative PCR Fast Kit

EpiQuik Quantitative PCR Fast Kit

Citations (5)

Input Type:	DNA
Research Area:	DNA Methylation
Target Application:	PCR Amplification
Vessel Format:	96-Well Plate, Columns/Tubes

P-1029-100	100 reactions	$204.00
P-1029-200	200 reactions	$364.00

Research Kits

MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric)

MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric)

Citations (335) | (5)

Input Type:	DNA
Research Area:	DNA Methylation
Target Application:	Amount Quantitation
Vessel Format:	96-Well Plate

P-1030-48	48 reactions	$338.00
P-1030-96	96 reactions	$612.00

Research Kits

MethylFlash Global DNA Hydroxymethylation (5-hmC) ELISA Easy Kit (Colorimetric)

MethylFlash Global DNA Hydroxymethylation (5-hmC) ELISA Easy Kit (Colorimetric)

Citations (82) | (1)

Input Type:	DNA
Research Area:	DNA Methylation
Target Application:	Amount Quantitation
Vessel Format:	96-Well Plate

P-1032-48	48 reactions	$360.00
P-1032-96	96 reactions	$633.00

Research Kits

MethylFlash Methylated DNA 5-mC Quantification Kit (Colorimetric)

MethylFlash Methylated DNA 5-mC Quantification Kit (Colorimetric)

Citations (472) | (6)

Input Type:	DNA
Research Area:	DNA Methylation
Target Application:	Amount Quantitation
Vessel Format:	96-Well Plate

P-1034-48	48 assays	$363.00
P-1034-96	96 assays	$669.00

Research Kits

MethylFlash Methylated DNA Quantification Kit (Fluorometric)

MethylFlash Methylated DNA Quantification Kit (Fluorometric)

Citations (72) | (1)

Input Type:	DNA
Research Area:	DNA Methylation
Target Application:	Amount Quantitation
Vessel Format:	96-Well Plate

P-1035-48	48 assays	$399.00
P-1035-96	96 assays	$699.00

Research Kits

MethylFlash Hydroxymethylated DNA 5-hmC Quantification Kit (Colorimetric)

MethylFlash Hydroxymethylated DNA 5-hmC Quantification Kit (Colorimetric)

Citations (105) | (1)

Input Type:	DNA
Research Area:	DNA Methylation
Target Application:	Amount Quantitation
Vessel Format:	96-Well Plate

P-1036-48	48 assays	$399.00
P-1036-96	96 assays	$699.00

Research Kits

MethylFlash Hydroxymethylated DNA Quantification Kit (Fluorometric)

MethylFlash Hydroxymethylated DNA Quantification Kit (Fluorometric)

Citations (16)

Input Type:	DNA
Research Area:	DNA Methylation
Target Application:	Amount Quantitation
Vessel Format:	96-Well Plate

P-1037-48	48 assays	$443.00
P-1037-96	96 assays	$743.00

Research Kits

EpiQuik Hydroxymethylated DNA Immunoprecipitation (hMeDIP) Kit

EpiQuik Hydroxymethylated DNA Immunoprecipitation (hMeDIP) Kit

Citations (48) | (2)

Input Type:	DNA
Research Area:	DNA Methylation
Target Application:	Immunoprecipitation
Vessel Format:	96-Well Plate

P-1038-24	24 reactions	$418.00
P-1038-48	48 reactions	$719.00
P-1038-96	96 reactions	$1,297.00

Research Kits

MethylFlash Urine 5-Methylcytosine (5-mC) Quantification Kit (Colorimetric)

MethylFlash Urine 5-Methylcytosine (5-mC) Quantification Kit (Colorimetric)

Input Type:	Urine
Research Area:	DNA Methylation
Target Application:	Amount Quantitation
Vessel Format:	96-Well Plate

P-1039-48	48 assays	$345.00
P-1039-96	96 assays	$599.00

Research Kits

MethylFlash Urine 5-Methylcytosine (5-mC) Quantification Kit (Fluorometric)

MethylFlash Urine 5-Methylcytosine (5-mC) Quantification Kit (Fluorometric)

Input Type:	Urine
Research Area:	DNA Methylation
Target Application:	Amount Quantitation
Vessel Format:	96-Well Plate

P-1040-48	48 assays	$345.00
P-1040-96	96 assays	$599.00

Research Kits

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Displaying 1 to 20 (of 49 products)

CUT&LUNCH Assay Kit

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