The DNA Methylation/Hydroxymethylation Standard Set is designed to be used as positive controls in DNA methylation experiments and various applications including MS/hMS-PCR and quantification of cytosine modifications in any species. It can also be used as substrates for studies involving 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) interacting proteins.
Fig. 1. Each DNA standard (500 ng) was run on a 2.5% agarose gel. All three DNA standards are 200 bp and either unmodified (cytosine, Lane 1), methylated (5-mC, Lane 2) or hydroxymethylated (5-hmC, Lane 3).
3 x 2 µg per standard (50 ng/ul)
Unmodified control primer (20 µM)Forward: 10 µlReverse: 10 ul
Modified control primer (20 µM)Forward: 10 µlReverse: 10 µl
Buzgoova K et. al. (April 2019). Brain derived neurotrophic factor expression and DNA methylation in response to subchronic valproic acid and/or aldosterone treatment. Croat Med J. 60(2):71-77.