Histone phosphorylation has been shown to impact an assortment of processes such as chromosome condensation, transcription, DNA repair, and apoptosis. An increase in phosphorylation is linked to gene activation and cell growth. Investigating the changes in histone phosphorylation levels using histone phosphorylation proteins such as Aurora Kinase A (AURKA) and MSK1 will aid researchers in understanding diseases and their pathological processes and can advance the creation of protein kinase-targeted drug development.