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Chromatin & Transcription

Chromatin studies, including chromatin immunoprecipitation (ChIP) and chromatin accessibility assays, are essential for understanding how DNA is packaged and regulated within the nucleus. These techniques help identify protein-DNA interactions, protein post-translational modifications, and accessible regulatory regions that influence gene expression, cell differentiation, and disease states.

Illustration of chromatin organization showing open, nuclease-accessible euchromatin and condensed, nuclease-resistant heterochromatin with nucleosomes.

Researchers study chromatin structure and dynamics to uncover mechanisms of gene regulation, epigenetic inheritance, and potential therapeutic targets for diseases.

Chromatin Immunoprecipitation Assays

Bar graph comparing DNA recovery percentages from DNA fragments of varying sizes (50 bp, 100 bp, 200 bp, 500 bp, 1 kb, 2 kb, and 5 kb), showing higher recovery efficiency for larger DNA fragment sizes.

Enrich for target protein-associated DNA sequences

CUT&RUN-Fast & More

Flowchart comparing traditional ChIP-seq and high-throughput ChIP-seq (HT ChIP-seq) methods, highlighting fewer steps, reduced complexity, and shorter processing time in the HT ChIP-seq workflow.

Improved DNA enrichment without the drawbacks of traditional ChIP and CUT&RUN

Chromatin Accessibility Assays

Bar graph displaying enrichment percentage (% Input) from ChIP assays comparing Histone H3-K4 methylation levels at GAPDH and MLH1 gene promoters in HeLa cells, indicating higher enrichment at GAPDH.

Assess open and closed chromatin configurations gene specifically or genome wide

In Vitro Protein-DNA Interaction Assays

Bar graph showing absorbance at 450 nm comparing DNA concentrations ranging from 0 to 200 ng, demonstrating a linear relationship with increasing absorbance as DNA concentration increases.

Measure activity and inhibition of HATs, HDACs, HMTs, and HDMTs

Chromatin Immunoprecipitation

Chromatin immunoprecipitation (ChIP) is a laboratory technique used to study the interaction between proteins and DNA in a specific region of the genome. It allows researchers to identify which proteins bind to specific DNA sequences and to determine the relative strength of these interactions. The basic principle of ChIP is to isolate chromatin (the complex of DNA and proteins that make up the chromosomes in cells) from cells or tissue, and then use an antibody specific to a particular protein of interest to pull down (precipitate) the DNA sequences that are bound to that protein. The precipitated DNA sequences can then be analyzed using techniques such as polymerase chain reaction (PCR) or DNA sequencing to identify the specific DNA sequences that are bound to the protein of interest. ChIP is a powerful tool for studying gene regulation and has been widely used to identify regulatory elements in the genome, including promoters, enhancers, and other regulatory elements that control gene expression. It has also been used to study the role of specific proteins in various biological processes, including development, differentiation, and disease. ChromaFlash High Sensitivity ChIP Kit

CUT&RUN-Fast and More

Chromatin immunoprecipitation (ChIP) is a popular tool for studying genome-wide protein/DNA interactions in cells, whereby target histone or transcription factor-complexed DNA is enriched, and the interaction regions are mapped, generally via next-generation sequencing. Due to the limitations of ChIP, mainly, 1) its requirement for large amounts of starting material, 2) long processing times, and 3) low resolution, Cleavage Under Target and Release Using Nuclease (CUT&RUN) emerged to address these issues. The technique, which is performed in situ on intact cells or nuclei without fixation, entails cleavage of chromatin at specific antibody-occupied sites by a pAG-MNase fusion protein and the subsequent release of protein/DNA complexes. CUT&RUN has several advantages over ChIP, including less starting material and higher resolution. However, the pAG-MNase fusion protein used in the procedure can generate non-specific cleavage by antibody un-coupled pAG-MNase, significantly limiting the specificity of CUT&RUN use for most transcription factors in different species and cell/tissue types and causing the target protein-interacted DNA region profiles to be seriously affected. In addition, CUT&RUN is still time-consuming. CUT&RUN-Fast and CUT&LUNCH An improved CUT&RUN technique, CUT&RUN-Fast, was developed by EpigenTek that employs a novel and unique nucleic acid cleavage enzyme mix, which has low sequence bias, to simultaneously fragment chromatin and cleave/remove any DNA sequences in both ends of the target protein/DNA complex without affecting DNA occupied by the target protein, thereby increasing specificity and resolution. This also greatly speeds up the process and avoids overnight incubation. Recently, EpigenTek has further refined the CUT&RUN-Fast method with the new Cleavage Under Target and Liberate Unique Nucleic Complex Homogenously (CUT&LUNCH), which features an extremely fast protocol, further enhanced specificity, and minimized background. Performing a CUT&LUNCH Experiment The CUT&LUNCH assay’s streamlined procedure allows you to selectively enrich target protein/DNA complexes very quickly. The rapid 3-step protocol can be completed in just under 2 hours, minimizing nuclear damage and chromatin loss while preserving native chromatin structure: Step 1: Antibody Binding to Target (30 minutes) Step 2: Enzyme Cleavage (10 minutes) Step 3: Selective Recovery and Purification (60 minutes) Advantages of CUT&LUNCH vs Traditional CUT&RUN and ChIP CUT&LUNCH Traditional CUT&RUN ChIP Workflow convenience Convenient

In Vitro Protein-DNA Interaction

Measurement of direct interactions between protein and DNA in vitro has an advantage in analyzing the binding of different transcription factors to specific DNA consensus sequences located in the gene promoters. By investigating protein-DNA interaction in vitro, it is possible to identify the genetic targets of DNA, which leads to a better understanding of cellular processes.

Chromatin Accessibility

Chromatin is the complex of DNA and proteins that make up the chromosomes in a cell's nucleus. Chromatin can be more or less accessible, depending on the presence of specific proteins and chemical modifications. When chromatin is highly accessible, it is more likely to be transcribed into RNA. When it is less accessible, transcription is less likely to occur. Measuring chromatin accessibility can be important for a variety of reasons: First, chromatin accessibility ...

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Displaying 1 to 20 (of 33 products)

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EpiQuik Chromatin Accessibility Assay Kit

Citations (32) | (2)

Input Type:	Cell Extracts, Chromatin, Unisolated Samples
Research Area:	Chromatin & Transcription
Target Application:	Sample Modification
Vessel Format:	Columns/Tubes

P-1047-48

48 reactions

$585.00

Research Kits

EpiNext ISAC Library Preparation Kit new product

Input Type:	Cleaved DNA
Research Area:	Chromatin & Transcription
Target Application:	Library Construction
Vessel Format:	Tubes

P-1057-24	24 Reactions	~~$699.00~~ $599.00
P-1057-48	2x24 Reactions	~~$1,199.00~~ $999.00

Research Kits

EpiNext CUT&LUNCH Library Preparation Kit new product

Input Type:	Enriched DNA
Research Area:	Chromatin & Transcription
Target Application:	Library Construction
Vessel Format:	Tubes

P-1058-24	24 Reactions	~~$699.00~~ $599.00
P-1058-48	2x24 Reactions	~~$1,199.00~~ $999.00

Research Kits

EpiNext Chromatin Accessibility Sequencing Fast Kit

Input Type:	Cells, Tissues
Research Area:	Chromatin & Transcription
Target Application:	Library Construction, Sample Modification
Vessel Format:	Tubes

P-1071-12	12 Reactions	~~$678.00~~ $611.00
P-1071-24	24 Reactions	~~$1,325.00~~ $1,192.00

Research Kits

EpiNext In Situ Accessible Chromatin (ISAC) Preparation Kit new product

Input Type:	Cells, Tissues
Research Area:	Chromatin & Transcription
Target Application:	Sample Modification
Vessel Format:	Tubes

P-1072-24	24 Reactions	~~$249.00~~ $199.00
P-1072-48	48 Reactions	~~$349.00~~ $299.00

Research Kits

EpiNext In Situ Accessible Chromatin Sequencing (ISAC-seq) Kit new product

Input Type:	Cells, Tissues
Research Area:	Chromatin & Transcription
Target Application:	Library Construction, Sample Modification
Vessel Format:	Tubes

P-1073-12	12 Reactions	~~$599.00~~ $499.00
P-1073-24	24 Reactions	~~$999.00~~ $899.00

Research Kits

EpiQuik Chromatin Immunoprecipitation (ChIP) Kit

Citations (278) | (2)

Input Type:	Chromatin
Research Area:	Chromatin & Transcription
Target Application:	Protein-DNA Enrichment
Vessel Format:	96-Well Plate

P-2002-1	24 reactions	$292.00
P-2002-2	48 reactions	$468.00
P-2002-3	96 reactions	$692.00

Research Kits

EpiQuik Tissue Chromatin Immunoprecipitation (ChIP) Kit

Citations (55)

Input Type:	Chromatin
Research Area:	Chromatin & Transcription
Target Application:	Protein-DNA Enrichment
Vessel Format:	96-Well Plate

P-2003-1	24 reactions	$292.00
P-2003-2	48 reactions	$468.00
P-2003-3	96 reactions	$692.00

Research Kits

EpiQuik General Protein-DNA Binding Assay Kit (Colorimetric)

Citations (15)

Input Type:	Nuclear Extracts
Research Area:	Chromatin & Transcription
Target Application:	Activity Measurement
Vessel Format:	96-Well Plate

P-2004-96

96 assays

$465.00

Research Kits

EpiQuik General Protein-DNA Binding Assay Kit (Fluorometric)

Citations (5)

Input Type:	Nuclear Extracts
Research Area:	Chromatin & Transcription
Target Application:	Activity Measurement
Vessel Format:	96-Well Plate

P-2005-96

96 assays

$469.00

Research Kits

EpiQuik Methyl-Histone H3K9 ChIP Kit

Citations (3)

Input Type:	Chromatin
Research Area:	Chromatin & Transcription
Target Application:	Protein-DNA Enrichment
Vessel Format:	96-Well Plate

P-2006-24	24 reactions	$423.00
P-2006-48	48 reactions	$670.00

Research Kits

EpiQuik Tri-Methyl-Histone H3K9 ChIP Kit

Citations (3)

Input Type:	Chromatin
Research Area:	Chromatin & Transcription
Target Application:	Protein-DNA Enrichment
Vessel Format:	96-Well Plate

P-2006T-24	24 reactions	$423.00
P-2006T-48	48 reactions	$670.00

Research Kits

EpiQuik Methyl-Histone H3K4 ChIP Kit

Citations (4)

Input Type:	Chromatin
Research Area:	Chromatin & Transcription
Target Application:	Protein-DNA Enrichment
Vessel Format:	96-Well Plate

P-2007-24	24 reactions	$399.00
P-2007-48	48 reactions	$657.00

Research Kits

EpiQuik Tissue Methyl-Histone H3K9 ChIP Kit

Citations (7)

Input Type:	Chromatin
Research Area:	Chromatin & Transcription
Target Application:	Protein-DNA Enrichment
Vessel Format:	96-Well Plate

P-2008-24	24 reactions	$423.00
P-2008-48	48 reactions	$670.00

Research Kits

EpiQuik Tissue Methyl-Histone H3K4 ChIP Kit

Citations (6)

Input Type:	Chromatin
Research Area:	Chromatin & Transcription
Target Application:	Protein-DNA Enrichment
Vessel Format:	96-Well Plate

P-2009-24	24 reactions	$423.00
P-2009-48	48 reactions	$670.00

Research Kits

EpiQuik Acetyl-Histone H3 ChIP Kit

Citations (21) | (1)

Input Type:	Chromatin
Research Area:	Chromatin & Transcription
Target Application:	Protein-DNA Enrichment
Vessel Format:	96-Well Plate

P-2010-24	24 reactions	$423.00
P-2010-48	48 reactions	$670.00

Research Kits

EpiQuik Acetyl-Histone H4 ChIP Kit

Citations (5)

Input Type:	Chromatin
Research Area:	Chromatin & Transcription
Target Application:	Protein-DNA Enrichment
Vessel Format:	96-Well Plate

P-2011-24	24 reactions	$423.00
P-2011-48	48 reactions	$670.00

Research Kits

EpiQuik Tissue Acetyl-Histone H3 ChIP Kit

Citations (10) | (1)

Input Type:	Chromatin
Research Area:	Chromatin & Transcription
Target Application:	Protein-DNA Enrichment
Vessel Format:	96-Well Plate

P-2012-24	24 reactions	$423.00
P-2012-48	48 reactions	$670.00

Research Kits

EpiQuik Tissue Acetyl-Histone H4 ChIP Kit

Citations (2)

Input Type:	Chromatin
Research Area:	Chromatin & Transcription
Target Application:	Protein-DNA Enrichment
Vessel Format:	96-Well Plate

P-2013-24	24 reactions	$423.00
P-2013-48	48 reactions	$670.00

Research Kits

EpiQuik Plant ChIP Kit

Citations (191) | (2)

Input Type:	Chromatin
Research Area:	Chromatin & Transcription
Target Application:	Protein-DNA Enrichment
Vessel Format:	96-Well Plate

P-2014-24	24 reactions	$359.00
P-2014-48	48 reactions	$593.00

Research Kits

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