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EpiQuik General Protein-DNA Binding Assay Kit (Colorimetric)

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Suggested Workflow
Nuclear Protein Extraction
 
 
In Vitro Protein-DNA Interaction Assay
 
 
PCR Analysis
 
Schematic procedure for using the EpiQuik General Protein-DNA Binding Assay Kit (Colorimetric).
Nuclear extracts treated with H2O2 as well as untreated extracts were prepared from MCF-7 cells and used in the detection of estrogen receptor-DNA binding with the EpiQuik™ General Protein-DNA Binding Assay Kit (Colorimetric).
Input Type: Nuclear Extracts
Research Area: Chromatin & Transcription
Target Application: Activity Measurement
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-2004-9696 assays $369.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

The EpiQuik™ General Protein-DNA Binding Assay Kit (Colorimetric) is a convenient package of tools that allows the experimenter to investigate protein-DNA interaction in vitro efficiently. The entire procedure can be completed within 3 hours and produces far more superior results than any competitor kits. The EpiQuik™ General Protein-DNA Binding Assay Kits are suitable for analyzing protein-DNA interactions in vitro, specifically for detecting transcription factor activation using mammalian tissue and cell extracts. The kit has the following advantages:

  • The fastest procedure available: a complete assay can be finished within 3 hours.
  • Strip microplate format makes the assay flexible: manual or high throughput analysis.
  • Colorimetrically quantifies protein activation and uses radioactive-free materials: safer to handle.
  • Simple, reliable, and consistent assay conditions.

Principle & Procedure
The EpiQuik™ General Protein-DNA Binding Assay Kit (Colorimetric) is designed for measuring the transcription factor of DNA binding activity in nuclear extracts. In an assay with the use of this kit, a biotin-labeled double stranded oligonucleotide (oligo) containing DNA binding consensus sequence for the target transcription factor is incubated with nuclear extract in the binding assay buffer. Active form of transcription factor in the nuclear extract binds to its consensus sequence. Oligo-protein complex is then captured onto the assay microwell. The target protein can be recognized by a high affinity antibody and colorimetrically measured through detection antibody-color development reagent reaction system.

 
Fig. 1. Schematic procedure for using the EpiQuik General Protein-DNA Binding Assay Kit (Colorimetric).

Fig. 2. Nuclear extracts treated with H2O2 as well as untreated extracts were prepared from MCF-7 cells and used in the detection of estrogen receptor-DNA binding with the EpiQuik™ General Protein-DNA Binding Assay Kit (Colorimetric).

Product Components

PD1 (10X Wash Buffer)
PD2 (Assay Binding Buffer)
PD3 (Antibody Dilution Buffer)
PD4 (Developing Solution)
PD5 (Stop Solution)
8-Well Assay Strips (with Frame)
User Guide

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]
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