The EpiQuik™ General Protein-DNA Binding Assay Kit (Fluorometric) is a convenient package of tools that allows the experimenter to investigate protein-DNA interaction in vitro efficiently. The entire procedure can be completed within 3 hours and produces far more superior results than any competitor kits. The EpiQuik™ General Protein-DNA Binding Assay Kits are suitable for analyzing protein-DNA interactions in vitro, specifically for detecting transcription factor activation using mammalian tissue and cell extracts. The kit has the following advantages:
Principle & ProcedureThe EpiQuik™ General Protein-DNA Binding Assay Kit (Fluorometric) is designed for measuring the transcription factor (TF) of DNA binding activity in nuclear extracts. In an assay using this kit, target TF in nuclear extract is captured by high affinity antibodies onto the assay microwells. A double strand oligonucleotide (oligo) containing DNA binding consensus sequence for the target TF is added into the microwell and bound to active TF in the binding assay buffer. The bound oligo is then fluorometrically detected.
Fig. 1. Schematic procedure for using the EpiQuik General Protein-DNA Binding Assay Kit (Fluorometric).
Fig. 2. Nuclear extracts treated with H2O2 as well as untreated extracts were prepared from MCF-7 cells and used in the detection of estrogen receptor-DNA binding with the EpiQuik™ General Protein-DNA Binding Assay Kit (Fluorometric).
Gao Y The Univer et. al. (June 2014). Up-regulation of Hepatic VLDLR via PPARα Is Required for the Triglyceride-Lowering Effect of Fenofibrate. J Lipid Res.
Herrera F et. al. (December 2010). Synergistic effect of retinoic acid and cytokines on the regulation of glial fibrillary acidic protein expression. J Biol Chem. 285(50):38915-22.
Yu Z et. al. (March 2010). CREB trans-activation of disruptor of telomeric silencing-1 mediates forskolin inhibition of CTGF transcription in mesangial cells. Am J Physiol Renal Physiol. 298(3):F617-24.