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EpiQuik General Protein-DNA Binding Assay Kit (Fluorometric)

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Suggested Workflow
Nuclear Protein Extraction
 
 
In Vitro Protein-DNA Interaction Assay
 
 
PCR Analysis
 
Schematic procedure for using the EpiQuik General Protein-DNA Binding Assay Kit (Fluorometric).
Nuclear extracts treated with H2O2 as well as untreated extracts were prepared from MCF-7 cells and used in the detection of estrogen receptor-DNA binding with the EpiQuik™ General Protein-DNA Binding Assay Kit (Fluorometric).
Input Type: Nuclear Extracts
Research Area: Chromatin & Transcription
Target Application: Activity Measurement
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-2005-9696 assays $380.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

The EpiQuik™ General Protein-DNA Binding Assay Kit (Fluorometric) is a convenient package of tools that allows the experimenter to investigate protein-DNA interaction in vitro efficiently. The entire procedure can be completed within 3 hours and produces far more superior results than any competitor kits. The EpiQuik™ General Protein-DNA Binding Assay Kits are suitable for analyzing protein-DNA interactions in vitro, specifically for detecting transcription factor activation using mammalian tissue and cell extracts. The kit has the following advantages:

  • The fastest procedure available: a complete assay can be finished within 3 hours.
  • Strip microplate format makes the assay flexible: manual or high throughput analysis.
  • Fluorometrically qualify or quantify protein activation and use radioactive-free materials: safer to handle.
  • No requirement for coating antibodies, or for using secondary antibodies, or for labeling oligo-nucleotides: save time and reduce labor.
  • Simple, reliable, and consistent assay conditions.

Principle & Procedure
The EpiQuik™ General Protein-DNA Binding Assay Kit (Fluorometric) is designed for measuring the transcription factor (TF) of DNA binding activity in nuclear extracts. In an assay using this kit, target TF in nuclear extract is captured by high affinity antibodies onto the assay microwells. A double strand oligonucleotide (oligo) containing DNA binding consensus sequence for the target TF is added into the microwell and bound to active TF in the binding assay buffer. The bound oligo is then fluorometrically detected.

 
Fig. 1. Schematic procedure for using the EpiQuik General Protein-DNA Binding Assay Kit (Fluorometric).

Fig. 2. Nuclear extracts treated with H2O2 as well as untreated extracts were prepared from MCF-7 cells and used in the detection of estrogen receptor-DNA binding with the EpiQuik™ General Protein-DNA Binding Assay Kit (Fluorometric).

Product Components

PF1 (10X Wash Buffer)
PF2 (Antibody Buffer)
PF3 (Extract Cleaner)*
PF4 (Assay Buffer)
PF5 (Oligo Release Solution)
PF6 (Fluoro Developer)*
DTT (500X)*
96-Well Assay Plate
8-Well Assay Strips (with Frame)
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]
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