EpiQuik In Vivo HDAC1 Sumoylation Assay Kit

The EpiQuik™ In Vivo HDAC1 Sumoylation Assay Kit is a convenient set of tools that allows the experimenter to measure in vivo HDAC1 sumoylation. The kit is ready-to-use and provides all the essential components needed for measuring in vivo HDAC1 sumoylation from multiple mammalian cells/tissues including human, mouse, and rat. The kit has the following advantages:

• Fast procedure, which can be finished within 3 hours.
• One-step colorimetric assay without the need for affinity chromatography and Western blotting.
• Included SUMO protein as the positive control allows HDAC1 sumoylation to be quantified.
• Strip microplate format makes the assay flexible: manual or high throughput analysis.
• Reliable and consistent assay conditions.

Principle & Procedure
The EpiQuik™ In Vivo HDAC1 Sumoylation Assay Kit is designed for measuring sumoylation of targeted proteins. Sumoylation of HDAC1 is indicated by SUMO conjugated to this protein. In assay with this kit, the antibody specific for the HDAC1 is stably coated on the strip wells. HDAC1 contained in the nuclear extracts is captured by the antibody. Sumoylation of HDAC1 can then be detected by recognition of SUMO conjugated to HDAC1 with an anti-SUMO antibody. The ratio or intensity of the sumoylation, which is proportional to the conjugated SUMO amount, can be quantified through the signal report-color development system.

 
Fig. 1. Schematic procedure for using the EpiQuik In Vivo HDAC1 Sumoylation Assay Kit.