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Methylated DNA Immunoprecipitation

Methylated DNA immunoprecipitation (meDIP) is a large scale antibody-based technique that is used to enrich and capture methylated DNA fragments for use in gene-specific DNA methylation studies on a genome wide scale. Following meDIP approaches, DNA methylation can be analyzed using a variety of downstream applications including meDIP-PCR, meDIP-ChIP, and meDIP-sequencing.


 

Methylamp Methylated DNA Capture (MeDIP) KitCat #P-1015

EpiQuik Hydroxymethylated DNA Immunoprecipitation (hMeDIP) KitCat #P-1038

EpiQuik MeDIP Ultra KitCat #P-1052

EpiQuik Methylated DNA Immunoprecipitation KitCat #P-2019

EpiQuik Tissue Methylated DNA Immunoprecipitation KitCat #P-2020

  More Details More Details More Details More Details More Details
Purpose Enrich and capture methylated DNA fragments Enrich and capture hydroxymethylated DNA fragments Enrich and capture methylated DNA fragments from low starting input Enrich and capture methylated DNA fragments directly from cell lysates Enrich and capture methylated DNA fragments directly from tissues
Key Points
  • Ideal for PCR and microarray
  • Illumina workflow compatible
  • Compatible with downstream workflows including hMeDIP-PCR and hMeDIP-chip
  • Ideal for MeDIP-Seq
  • Illumina workflow compatible
  • Most sensitive and specific
  • Eliminates DNA
    isolation step by immunoprecipitating meDNA fragments directly from cells
  • Eliminates DNA
    isolation step by immunoprecipitating meDNA fragments directly from tissues
Starting Material DNA DNA DNA Cells Tissues
Lowest Input Amount 0.2-1 µg 0.1 µg 50 ng DNA
(10,000 cells)
100,000 cells 10 mg
Total Protocol Time < 3 hours < 3 hours < 3 hours < 4 hours < 3.5 hours
Enrichment Efficiency > 98% > 99% > 99% > 95% > 95%
Conversion Efficiency 99.9% 99.9% 99.9% 99.9% 99.9%
Highly Specific Antibody Included
Columns for DNA Purification Included  
Vessel Format 96-Well Plate 96-Well Plate 96-Well Plate 96-Well Plate 96-Well Plate
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