Sitewide 2017 Summer Sale Site-wide summer sale! Take 10% off your entire order when using code E1707SUN. » Learn More
Epigentek Home
 
Epigentek Free Shipping

EpiQuik Methylated DNA Immunoprecipitation Kit

Share:

Citations (12) | Write a Review
Suggested Workflow
DNA Shearing
 
 
MeDIP
 
 
PCR Analysis
 
Schematic procedure for using the EpiQuik EpiQuik Methylated DNA Immunoprecipitation Kit.
Input Type: Cells
Research Area: DNA Methylation
Target Application: Immunoprecipitation
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-2019-2424 reactions $338.00 
P-2019-4848 reactions $549.00 
Availability: Usually Ships in 1 Day or Same day delivery Same Day NY Delivery 
Product Overview

Please see the Methylamp DNA Capture Kit if you are working with purified DNA as the starting material. 


The EpiQuik™ Methylated DNA Immunoprecipitation (MeDIP) Kit is a complete set of essential components which uses a proprietary and unique procedure/composition to enrich methylated DNA from cells. In the assay, an antibody specific to methyl cytosine is used to immunoprecipitate methylated genomic DNA. The immunoprecipitated methylated fractions can be then used for a standard DNA detection. The kit is suitable for combining the specificity of methylated DNA immunoprecipitation with qualitative and quantitative PCR, and southern blot as well as DNA microarray. The kit has the following advantages:

  • Directly immunoprecipitate the methylated fractions of DNA from cell lysates.
  • Highly efficient enrichment of methylated DNA: > 95%.
  • Strip microplate format allows manual or high throughput.
  • Conveniently includes columns for DNA purification in order to save time and reduce labor.
  • Compatible with all DNA amplification-based approaches.
  • Achieves very reliable and consistent assay conditions.

Principle & Procedure
The EpiQuik™ Methylated DNA Immunoprecipitation (MeDIP) Kit contains all reagents required for carrying out a successful methylated DNA immunoprecipitation directly from mammalian cells. Particularly, this kit includes a ChIP-grade 5-methylcytosine antibody and a negative control normal mouse IgG. DNA in the cells is extracted, sheared, and added into the microwell immobilized with the antibody. DNA is released from the antibody-DNA complex, and purified through the specifically designed Fast-Spin Column. Eluted DNA can be used for various down-stream applications.

Product Components

CP1 (Wash Buffer)
CP2 (Antibody Buffer)
CP3A (Pre-Lysis Buffer)
CP3B (Lysis Buffer)
CP4 (ChIP Dilution Buffer)
CP5 (DNA Release Buffer)
CP6 (Reverse Buffer)
CP7 (Binding Buffer)
CP8 (Elution Buffer)
Normal Mouse IgG (1 mg/ml)*
Anti-5-Methylcytosine (1 mg/ml)*
Proteinase K (10 mg/ml)*
8-Well Assay Strips (with Frame)
8-Well Strip Caps
F-Spin Column
F-Collection Tube
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

Frequently Asked Q's

1. What is the difference between the Methylamp Methylated DNA Capture Kit (#P-1015) and the EpiQuik Methylated DNA Immunoprecipitation Kit (#P-2019)?
The Methylamp Methylated DNA Capture Kit is for purified DNA as the starting materials while the EpiQuik Methylated DNA Immunoprecipitation Kit is for nuclear extract as the starting materials. Also, kit P-2019 is specifically designed for immunoprecipitating meDNA fraction directly from cells. The main advantage of P-2019/2020 is that no pre-isolation/purification of DNA is required if mammalian cells/tissues are used.

2. If I am using kit P-2019 starting with purified DNA rather than cells, do I have to modify any steps in the protocol?
When using kit P-2019, purified DNA cannot be used as starting material. The washing buffer, lysis buffer, and reversing buffer in the kit are not suitable for purified DNA.

3. How can I enrich 200 ng of mDNA, as required for a microarray?
Pool the IPed mDNA from 10-20 wells and then purify the mDNA with 5-10 columns (2 wells/each column). It is also useful to generate sufficient DNA for a microarray by amplifying IP’ed mDNA using whole genome amplification (WGA).

4. On page 10 of the user guide, does the "input" vial need to add 150 µl CP7 as the samples do?
Yes, CP7 should also be added into the "input" vial for DNA binding to the column.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]
Product Citations

He Z et. al. (March 2017). <sup>TRIM36 hypermethylation is involved in polycyclic aromatic hydrocarbons-induced cell transformation</sup>. Environ Pollut. 225:93-103.

Arash Pooladi et. al. (September 2016). Potential Use of DOK7 Gene Methylation Level in the Peripheral Blood Cells as a Biomarker in Early Diagnosis of Sporadic Breast Cancer G3M. 14(3):4310-4317.

Yu HL et. al. (March 2015). Global DNA methylation was changed by a maternal high-lipid, high-energy diet during gestation and lactation in male adult mice liver. Br J Nutr. :1-8.

Chen J et. al. (January 2015). Association between DNA methylation and multidrug resistance in human glioma SHG-44 cells. Mol Med Rep. 11(1):43-52.

Michailidi C et. al. (April 2014). Genome-wide and gene-specific epigenomic platforms for hepatocellular carcinoma biomarker development trials. Gastroenterol Res Pract. 2014:597164.

Bellizzi D et. al. (December 2013). The control region of mitochondrial DNA shows an unusual CpG and non-CpG methylation pattern. DNA Res. 20(6):537-47.

Calabrese F et. al. (August 2013). Lack of serotonin transporter alters BDNF expression in the rat brain during early postnatal development. Mol Neurobiol. 48(1):244-56.

Lomniczi A et. al. (March 2013). Epigenetic control of female puberty. Nat Neurosci. 16(3):281-9.

Glait-Santar C et. al. (August 2012). Expression pattern of SVEP1 alternatively-spliced forms. Gene. 505(1):137-45.

Glait-Santar C et. al. (December 2011). SVEP1 promoter regulation by methylation of CpG sites. Gene. 490(1-2):6-14.

Schwarz JM et. al. (December 2011). Early-life experience decreases drug-induced reinstatement of morphine CPP in adulthood via microglial-specific epigenetic programming of anti-inflammatory IL-10 expression. J Neurosci. 31(49):17835-47.

Bechtel W et. al. (May 2010). Methylation determines fibroblast activation and fibrogenesis in the kidney. Nat Med. 16(5):544-50.

Epigentek Rewards
Related Products

Researchers Also Purchased
EpiQuik Hydroxymethylated DNA Immunoprecipitation (hMeDIP) Kit
EpiQuik Hydroxymethylated DNA Immunoprecipitation (hMeDIP) Kit
EpiQuik Tissue Methylated DNA Immunoprecipitation Kit
EpiQuik Tissue Methylated DNA Immunoprecipitation Kit
Methylamp Methylated DNA Capture (MeDIP) Kit
Methylamp Methylated DNA Capture (MeDIP) Kit
EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit
EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit
Epigentek is an epigenetics company that specializes in epigenetic kits, antibodies, reagents, and services for epigenetic research in DNA methylation, histone modification, and chromatin studies. Terms & Conditions | Privacy Policy | Site Map
Copyright © 2017 Epigentek Group Inc. All rights reserved.