Save $100 on Antibodies Save $100 when you buy $500 in antibodies. Use code E1705ABR. Expires June 30th. » Learn More
Epigentek Home
 
Epigentek Free Shipping

EpiQuik Tissue Methylated DNA Immunoprecipitation Kit

Share:

Citations (12) | Write a Review
Suggested Workflow
DNA Shearing
 
 
MeDIP
 
 
PCR Analysis
 
Schematic procedure for using the EpiQuik Tissue Methylated DNA Immunoprecipitation Kit.
Input Type: Tissues
Research Area: DNA Methylation
Target Application: Immunoprecipitation
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-2020-2424 reactions $338.00 
P-2020-4848 reactions $549.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

The EpiQuik™ Tissue Methylated DNA Immunoprecipitation (MeDIP) Kit is a complete set of essential components which uses a proprietary and unique procedure/composition to enrich methylated DNA from various mammalian tissues. In the assay, an antibody specific to methyl cytosine is used to immunoprecipitate methylated genomic DNA. The immunoprecipitated methylated fractions can be then used for a standard DNA detection. The kit is suitable for combining the specificity of methylated DNA immunoprecipitation with qualitative and quantitative PCR, and southern blot as well as DNA microarray. The kit has the following advantages:

  • Directly immunoprecipitate methylated fractions of DNA from tissue lysates.
  • Highly efficient enrichment of methylated DNA: > 95%
  • The fastest procedure available, which can be finished within 3 and a half hours.
  • Strip microwell format makes the assay flexible: manual or high throughput.
  • Columns for DNA purification are included: save time and reduce labor.
  • Compatible with all DNA amplification-based approaches.
  • Simple, reliable, and consistent assay conditions.

Principle & Procedure
The EpiQuik™ Tissue Methylated DNA Immunopricipitation (MeDIP) Kit contains all reagents required for carrying out a successful methylated DNA immunoprecipitation directly from mammalian tissues. Particularly, this kit includes a ChIP-grade 5-methylcytosine antibody and a negative control normal mouse IgG. DNA in the cells is extracted, sheared, and added into the microwell immobilized with the antibody. DNA is released from the antibody-DNA complex, and purified through the specifically designed Fast-Spin Column. Eluted DNA can be used for various down-stream applications.

 
Fig. 1. Schematic procedure for using the EpiQuik Tissue Methylated DNA Immunoprecipitation Kit.

Product Components

CP1 (Wash Buffer)
CP2 (Antibody Buffer)
CP3 (Lysis Buffer)
CP4 (ChIP Dilution Buffer)
CP5 (DNA Release Buffer)
CP6 (Reverse Buffer)
CP7 (Binding Buffer)
CP8 (Elution Buffer)
Homogenizing Buffer
Normal Mouse IgG (1 mg/ml)*
Anti-5-Methylcytosine (1 mg/ml)*
Proteinase K (10 mg/ml)*
8-Well Assay Strips (with Frame)
8-Well Strip Caps
F-Spin Column
F-Collection Tube
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

Frequently Asked Q's

1. If I am using kit P-2020, starting with purified DNA rather than cells, would I have to modify any steps in the protocol?
When using kit P-2020, purified DNA cannot be used as starting material. The washing buffer, lysis buffer, and reversing buffer in the kit are not suitable for purified DNA.

2. What are the differences between the EpiQuik Methylated DNA IP Kit (#P-2020) and the MethylAmp Methyl DNA Capture Kit (#P-1015)?
Kit P-2020 is specifically designed for immunoprecipitating mDNA fraction directly from tissues. The main advantage of P-2019/2020 is that no pre-isolation/purification of DNA is required if mammalian cells/tissues are used.

3. On page 10 of the user guide, does the "input" vial need to add 150 ul CP7 as the samples do?
Yes, CP7 should also be added into the "input" vial for DNA binding to the column.

4. Why would WGA amplify both DNA from sample and control IgG?
10% background from IgG precipitatation, after WGA, it will also be amplified. It is not necessary for microarray use. Input should be used.

5. How can I enrich 1-4µg of DNA, as required for microarray?
Amplify IPed DNA by WGA.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]
Product Citations

Wu GC et. al. (April 2016). The Testis Is a Primary Factor That Contributes to Epigenetic Modifications in the Ovaries of the Protandrous Black Porgy, Acanthopagrus schlegelii. Biol Reprod.

Hong S et. al. (January 2015). Epigenetic regulation of genes that modulate chronic stress-induced visceral pain in the peripheral nervous system. Gastroenterology. 148(1):148-157.e7.

Tyagi E et. al. (January 2015). Interactive actions of Bdnf methylation and cell metabolism for building neural resilience under the influence of diet. Neurobiol Dis. 73:307-18.

Anier K et. al. (March 2014). Maternal separation is associated with DNA methylation and behavioural changes in adult rats. Eur Neuropsychopharmacol. 24(3):459-68.

Anier K et. al. (October 2013). S-adenosylmethionine modifies cocaine-induced DNA methylation and increases locomotor sensitization in mice. Int J Neuropsychopharmacol. 16(9):2053-66.

Calabrese F et. al. (August 2013). Lack of serotonin transporter alters BDNF expression in the rat brain during early postnatal development. Mol Neurobiol. 48(1):244-56.

Zhang X et. al. (January 2013). Promoter hypermethylation of ARID1A gene is responsible for its low mRNA expression in many invasive breast cancers. PLoS One. 8(1):e53931.

Anier K et. al. (November 2010). DNA methylation regulates cocaine-induced behavioral sensitization in mice. Neuropsychopharmacology. 35(12):2450-61.

Aoyama T et. al. (September 2010). Histone modifiers, YY1 and p300, regulate the expression of cartilage-specific gene, chondromodulin-I, in mesenchymal stem cells. J Biol Chem. 285(39):29842-50.

Bechtel W et. al. (May 2010). Methylation determines fibroblast activation and fibrogenesis in the kidney. Nat Med. 16(5):544-50.

Molteni R et. al. (March 2010). Reduced function of the serotonin transporter is associated with decreased expression of BDNF in rodents as well as in humans. Neurobiol Dis. 37(3):747-55.

Roth TL et. al. (May 2009). Lasting epigenetic influence of early-life adversity on the BDNF gene. Biol Psychiatry. 65(9):760-9.

Epigentek Product Reviews
Related Products

Researchers Also Purchased
EpiQuik Methylated DNA Immunoprecipitation Kit
EpiQuik Methylated DNA Immunoprecipitation Kit
5-Methylcytosine (5-mC) Monoclonal Antibody [33D3]
5-Methylcytosine (5-mC) Monoclonal Antibody [33D3]
MethylFlash Methylated DNA 5-mC Quantification Kit (Colorimetric)
MethylFlash Methylated DNA 5-mC Quantification Kit (Colorimetric)
EpiQuik Tissue Chromatin Immunoprecipitation (ChIP) Kit
EpiQuik Tissue Chromatin Immunoprecipitation (ChIP) Kit
Epigentek is an epigenetics company that specializes in epigenetic kits, antibodies, reagents, and services for epigenetic research in DNA methylation, histone modification, and chromatin studies. Terms & Conditions | Privacy Policy | Site Map
Copyright © 2017 Epigentek Group Inc. All rights reserved.