Rapidly enrich and capture methylated or hydroxymethylated DNA
Let’s get to the point. If you’re looking for a quick and efficient way of identifying cytosine modifications in your DNA sample, then EpiGentek’s highly specific MeDIP and hMeDIP kits are right for you. Using superior quality antibodies with strong affinity to 5mC or 5hmC and without cross-reactivity to other cytosines, our kits accurately enrich and capture methylated or hydroxymethylated DNA using a convenient 96-well format. The immunoprecipitated DNA fractions can then be used in standardized DNA detection methods, such as PCR, microarrays, and sequencing.
Schematic procedure for the Methylamp Methylated DNA Capture (MeDIP) Kit (Cat# P-1015).
About Methylated DNA Immunoprecipitation
MeDIP is a genome-wide, large-scale technique to enrich and capture methylated DNA by isolating methylated DNA fragments with a 5-methylcytosine (5-mC) antibody. This technique has played an important role in helping to identify abnormally methylated genes in diseases such as cancer. Similarly, hydroxymethylated DNA can be isolated (hMeDIP) using a 5-hydroxymethylcytosine (5-hmC) antibody. The immunoprecipitated fractions can then be assessed by PCR to evaluate the methylation/hydroxymethylation state of individual regions. Additionally, the purified fractions can be input to high throughput DNA detection methods such as DNA microarrays (MeDIP-chip) and next-generation sequencing (MeDIP-seq), thus becoming a valuable approach for methylome-level analysis and for developing comprehensive profiles of DNA methylation on a genome-wide scale.
Fast: The entire procedure can be finished within 3 hours
Highly Efficient: Enrichment of methylated DNA: > 98%
Convenient: Contains all reagents required to enrich and capture methylated DNA fragments and compatible with all DNA amplification-based approaches
Flexible: Strip-well microplate format makes the assay flexible: manual or high throughput
Catalog #: P-1015
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EpiQuik MeDIP Ultra Kit
For antibody-based capture of methylated DNA fragments for microarray, PCR, or NGS applications
Fast: Total protocol time is less than 3 hours
Highly Efficient: Enrichment of methylated DNA with >99% specificity
Convenient: Contains all reagents required to enrich and capture methylated DNA fragments and compatible with various downstream analysis workflows including MeDIP-PCR, MeDIP-chip, and MeDIP-seq
Flexible: Strip-well microplate format makes the assay flexible: manual or high throughput
Highly Efficient: Enrichment ratio of positive/negative control > 100
Convenient: Contains all reagents required to enrich and capture methylated DNA fragments and compatible various downstream analysis workflows including hMeDIP-PCR and hMeDIP-chip
Flexible: Strip-well microplate format makes the assay flexible: manual or high throughput