Global 5-hmC Quantification

EpiGentek’s ELISA-based assay service for global 5-hydroxymethylcytosine (5-hmC) quantification measures total genome-wide hydroxymethylated cytosine levels in DNA. This method is highly specific, separately detecting 5-hydroxymethylcytosine with no cross-reactivity to methylcytosine and unmethylated cytosine. By measuring global DNA hydroxymethylation status, researchers can investigate the distribution of this base (5-hmC) in various cell types and in different areas of the mammalian genome. Being able to identify hydroxymethylation status in human cells/tissues is particularly important when comparing diseased and healthy cells.

Global 5-hmC
Service #S-2GHQ
Quantitate total hydroxymethylated cytosine levels in DNA on a global scale.
Antibody-based processing of samples
Accurate readout of OD values
Calculation of total global 5-hmC levels

Sample Requirements

Epigenetic Services Sample Requirements
We currently accept DNA, cell, and tissue samples for our Global 5-hmC Quantification service. Proper sample preparation along with the appropriate quality control methods on your end will allow the greatest chance for assay success. Please follow all guidelines for sample requirements, packaging and labeling, and shipping which are outlined on the Submission Instructions page.

Talk to a Scientist

Want more information on our Global 5-hmC Quantification service or interested in customizing your research project? We’re here to help! Get in touch with one of our highly knowledgeable epigenetic services experts by calling toll-free at 1-877-374-4368 or emailing services @