DNA Methylation Quantification

DNA methylation plays an important role in normal organismal development and in cellular differentiation in higher organisms. Gene expression, as well as the development of nearly all types of cancer, are also tied to DNA methylation. For example, global decrease in 5-methylcytosine content (DNA hypomethylation) is likely caused by methyl-deficiency due to a variety of environmental influences, and has been proposed as a molecular marker in multiple biological processes such as cancer. Global quantification of DNA methylation is crucial for understanding the roles that gene expression and silencing play in the development of cancer and other diseases.

	MethylFlash Methylated DNA Quantification Kit (Colorimetric)Cat #P-1034	MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric)Cat #P-1030	MethylFlash Global DNA Hydroxymethylation (5-hmC) ELISA Easy Kit (Colorimetric)Cat #P-1032	MethylFlash 5-Formylcytosine (5-fC) DNA Quantification Kit (Colorimetric)Cat #P-1041
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Purpose	Colorimetrically measure global 5mC in DNA	Colorimetrically measure global 5mC in DNA	Colorimetrically measure global 5hmC in DNA	Colorimetrically measure global 5fC in DNA
Key Points	Highly cited method in major journals Comparable results to HPLC-MS	Reduced cross-variation and improved accuracy Fast and convenient "one-step" assay reaction	Robust 4th-generation MethylFlash technology applied to 5hmC studies	Robust MethylFlash technology applied to 5fC studies
Starting Material	DNA	DNA	DNA	DNA
Input Range	50-200 ng	20-200 ng	50-200 ng	100-300 ng
Optimal Input Amount	100 ng	100 ng	100 ng	300 ng
Total Time	4 hours	2 hours	2 hours	4 hours
Detection Threshold	200 pg of meDNA	50 pg of meDNA	10 pg of hMeDNA	1 pg of fC DNA
Type of Methylation Detected	5-methylcytosine	5-methylcytosine	5-hydroxymethylcytosine	5-formylcytosine
Specificity	>1000:1 meDNA:unMeDNA	>1000:1 meDNA:unMeDNA	>1000:1 hMeDNA:unMeDNA	>1000:1 fC DNA:unMeDNA
Format	ELISA-like Microplate-based	ELISA-like Microplate-based	ELISA-like Microplate-based	ELISA-like Microplate-based