The EpiQuik™ MBD2 Binding Activity/Inhibition Assay Kit is a convenient set of tools that allows the experimenter to measure binding activity of MBD2 to methylated DNA. The kit is ready-to-use and provides all the essential components needed to carry out a successful activity/inhibition experiment without the need for radioactivity or any special equipment. The EpiQuik™ MBD2 Binding Activity Assay Kit is suitable for measuring MBD2 binding activity in human cells/tissues. The kit has the following advantages:
Principle & ProcedureThe EpiQuik™ MBD2 Binding Activity Assay Kit is designed for measuring in vitro or intracellular MBD2 binding activity. In an assay with this kit, activated MBD2 protein contained in nuclear extract binds to methylated DNA and forms the MBD2/methylated DNA complex. The complex is then captured to a specifically-treated strip well plate. MBD2 is recognized with a specific antibody and measured colorimetrically.
Fig. 1. Schematic procedure for using the EpiQuik MBD2 Binding Activity/Inhibition Assay Kit.
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