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EpiQuik MBD2 Binding Activity/Inhibition Assay Kit

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Suggested Workflow
Nuclear Protein Extraction
 
 
Demethylase Assay
 
Schematic procedure for using the EpiQuik MBD2 Binding Activity/Inhibition Assay Kit.
Input Type: DNA
Research Area: DNA Methylation
Target Application: Activity Measurement
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-3021-4848 assays $369.00 
P-3021-9696 assays $549.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

The EpiQuik™ MBD2 Binding Activity/Inhibition Assay Kit is a convenient set of tools that allows the experimenter to measure binding activity of MBD2 to methylated DNA. The kit is ready-to-use and provides all the essential components needed to carry out a successful activity/inhibition experiment without the need for radioactivity or any special equipment. The EpiQuik™ MBD2 Binding Activity Assay Kit is suitable for measuring MBD2 binding activity in human cells/tissues. The kit has the following advantages:

  • The fastest procedure available, which can be finished within 3 hours.
  • Innovative colorimetric assay to quantitatively measure MBD2 binding.
  • Strip microplate format makes the assay flexible: manual or high throughput analysis.
  • Simple, reliable, and consistent assay conditions.

Principle & Procedure
The EpiQuik™ MBD2 Binding Activity Assay Kit is designed for measuring in vitro or intracellular MBD2 binding activity. In an assay with this kit, activated MBD2 protein contained in nuclear extract binds to methylated DNA and forms the MBD2/methylated DNA complex. The complex is then captured to a specifically-treated strip well plate. MBD2 is recognized with a specific antibody and measured colorimetrically.

 
Fig. 1. Schematic procedure for using the EpiQuik MBD2 Binding Activity/Inhibition Assay Kit.

Product Components

MB1 (10X Wash Buffer)
MB2 (Assay Buffer)
MB3 (Methylated DNA, 25 µg/ml)*
MB4 (Affinity Antibody, 50 µg/ml)*
MB5 (Detection Antibody, 200 µg/ml)*
MB6 (Developing Solution)
MB7 (Stop Solution)
8-Well Assay Strips (with frame)
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]
Replace Your Santa Cruz Abs
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