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   Home  »  Research Kits  »  Histone Acetylation  »  Histone Acetyltransferase (HAT) Assay  »  EpiQuik HAT Activity/Inhibition Assay Kit 
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Base Cat #P-4003

EpiQuik HAT Activity/Inhibition Assay Kit

Write a Review|96 Citations

Suggested Workflow
Nuclear Protein Extraction
 
 
HAT Assay
 
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▼
Schematic procedure for using the EpiQuik HAT Activity/Inhibition Assay Kit.
Illustrated standard curve generated with HAT assay standard.
Nuclear proteins were prepared from different cell lines and HAT activity was measured using the EpiQuik™ HAT Activity/Inhibition Assay Kit.
Input Type: Nuclear Extracts, Purified Enzymes
Research Area: Histone Acetylation
Target Application: Activity Measurement
Vessel Format: 96-Well Plate
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Catalog No.SizePriceQty
P-4003-4848 assays $269.00 
P-4003-9696 assays $470.00 
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Product Overview

The EpiQuik™ HAT Activity/Inhibition Assay Kit is a ready-to-use set of essential components needed to measure histone acetyltransferase activity/inhibition safely and quickly. It is suitable for measuring histone acetyltransferase activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria. The kit has the following advantages:

  • Very quick procedure, which can be completed within only 3 hours.
  • Innovative colorimetric assay without radioactivity, extraction, and chromatography.
  • Direct measurement of HAT activity and inhibition by quantifying the amount of acetylated histone substrate, thereby avoiding the false inhibitory effect on HATs.
  • Strip microplate format makes the assay flexible in both manual and high throughput analysis formats.
  • Simple, reliable, and consistent assay conditions.

Principle & Procedure
The EpiQuik™ HAT Activity/Inhibition Assay Kit is designed for measuring total histone aceyltransferase activity or inhibition. In an assay with this kit, the unique histone substrate is stably captured on the strip wells. Active HATs bind to and acetylate histone substrate. The acetylated substrate can be recognized with a high affinity anti-acetylated histone antibody. The ratio or amount of the acetylated histone, which is directly proportional to HAT enzyme activity, can then be colorimetrically quantified through an ELISA-like reaction.

Datasheet & SDS

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Datasheet/User Guide: P-4003.pdf If the above file is not available online, you can request a copy by contacting us.
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Product Citations

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  5. Paz NE et. al. (September 2025). Targeting histone acetylation to enhance somatic embryogenesis in Quercus suber L. Tree Physiol.
View all publications (96)
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  3. Lumpp T et. al. (January 2026). Impact of Arsenite on Transient and Persistent Histone H3 Modifications and Transcriptional Response. Chem Res Toxicol.
  4. Shang T et. al. (October 2025). The noncanonical function of liver-type phosphofructokinase potentiates the efficacy of HDAC inhibitors in cancer. Signal Transduct Target Ther. 10(1):341.
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