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Base Cat #P-3019

EpiQuik DNA Demethylase Activity/Inhibition Assay Kit

Suggested Workflow
Nuclear Protein Extraction
 
 
Demethylase Assay
 
We recommend using an alternative (Catalog #P-3008): EpiQuik DNA Demethylase Activity/Inhibition Assay Ultra Kit
Input Type: Nuclear Extracts, Purified Enzymes
Research Area: DNA Methylation
Target Application: Activity Measurement
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Application Guarantee for AntibodiesRecombinant Rabbit Monoclonal Abs
Catalog No.SizePriceQty
P-3019-4848 assays $470.00 
P-3019-9696 assays $699.00 
Order now & get it by Wednesday, September 30th  

Product Overview

The EpiQuikâ„¢ DNA Demethylase Activity/Inhibition Assay Kit is a convenient package of tools that allows the experimenter to measure DNA demethylase activity/inhibition at tremendously fast speeds and consistency; superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed for measuring total DNA demethylase activity/inhibition. The EpiQuikâ„¢ DNA Demethylase Activity/Inhibition Assay Kit is suitable for measuring DNA demethylase activity/inhibition using purified enzyme or protein extracts from the cultured cells and tissues.

WHY CHOOSE THE EPIQUIKâ„¢ DNA DEMETHYLASE ACTIVITY/INHIBITION ASSAY KIT,

  • Quick and efficient procedure, which can be finished within 3 hours.
  • Innovative colorimetric assay without the need for radioactivity, electrophoresis, and chromatography.
  • Strip microwell format makes the assay flexible: manual or high throughput.
  • Simple, reliable, and consistent assay conditions.

Product Details

The EpiQuik™ DNA Demethylase Activity/Inhibition Assay Kit is designed for measuring total DNA demethylase activity/inhibition. In an assay with this kit, the unique methylated DNA substrate is stably captured on the strip wells. Active DNA demethylases bind to and demethylate DNA substrate. The methylated DNA can be recognized with a high affinity 5-methycytosine antibody. The ratio or amount of methylated DNA, which is inversely proportional to enzyme activity, can then be colorimetrically quantified through an ELISA-like reaction.


SCHEMATIC PROCEDURE:



Nuclear extracts were prepared from MCF-7 cells using the EpiQuik Nuclear Extraction Kit and total DNA demethylase activity was measured.

Datasheet & SDS

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