Sitewide 2017 Summer Sale Site-wide summer sale! Take 10% off your entire order when using code E1707SUN. » Learn More
Epigentek Home
 
Epigentek Free Shipping

EpiQuik DNA Demethylase Activity/Inhibition Assay Kit

Share:

Citations (1) | Write a Review
Suggested Workflow
Nuclear Protein Extraction
 
 
Demethylase Assay
 
Schematic procedure for the SuperSense Methylated DNA Quantification Kit.
Nuclear extracts were prepared from MCF-7 cells using the EpiQuik Nuclear Extraction Kit and total DNA demethylase activity was measured.
Input Type: Nuclear Extracts, Purified Enzyme
Research Area: DNA Methylation
Target Application: Activity Measurement
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-3019-4848 assays $369.00 
P-3019-9696 assays $549.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

 

 

We recommend using a newer version of this product: EpiQuik DNA Demethylase Activity/Inhibition Assay Ultra Kit



The EpiQuik™ DNA Demethylase Activity/Inhibition Assay Kit is a convenient package of tools that allows the experimenter to measure DNA demethylase activity/inhibition at tremendously fast speeds and consistency; superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed for measuring total DNA demethylase activity/inhibition. The EpiQuik™ DNA Demethylase Activity/Inhibition Assay Kit is suitable for measuring DNA demethylase activity/inhibition using purified enzyme or protein extracts from the cultured cells and tissues.

WHY CHOOSE THE EPIQUIK™ DNA DEMETHYLASE ACTIVITY/INHIBITION ASSAY KIT,

  • Quick and efficient procedure, which can be finished within 3 hours.
  • Innovative colorimetric assay without the need for radioactivity, electrophoresis, and chromatography.
  • Strip microwell format makes the assay flexible: manual or high throughput.
  • Simple, reliable, and consistent assay conditions.
Product Details

The EpiQuik™ DNA Demethylase Activity/Inhibition Assay Kit is designed for measuring total DNA demethylase activity/inhibition. In an assay with this kit, the unique methylated DNA substrate is stably captured on the strip wells. Active DNA demethylases bind to and demethylate DNA substrate. The methylated DNA can be recognized with a high affinity 5-methycytosine antibody. The ratio or amount of methylated DNA, which is inversely proportional to enzyme activity, can then be colorimetrically quantified through an ELISA-like reaction.


SCHEMATIC PROCEDURE:



Nuclear extracts were prepared from MCF-7 cells using the EpiQuik Nuclear Extraction Kit and total DNA demethylase activity was measured.
Product Components

DD1 (10X Wash Buffer)
DD2 (Demethylase Assay Buffer)
DD3 (Demethylase Substrate)*
DD4 (Demethylation Standard, 10 µg/ml)*
DD5 (Capture Antibody, 1000 µg/ml)*
DD6 (Detecting Antibody, 200 µg/ml)*
DD7 (Developing Solution)
DD8 (Stop Solution)
8-Well Assay Strips (with Frame)
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]
Product Citations

Horrillo A et. al. (April 2013). Zebularine regulates early stages of mESC differentiation: effect on cardiac commitment. Cell Death Dis. 4:e570.

Replace Your Santa Cruz Abs
Related Products

Researchers Also Purchased
EpiQuik Nuclear Extraction Kit
EpiQuik Nuclear Extraction Kit
EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit
EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit
EpiQuik HDAC Activity/Inhibition Assay Kit (Colorimetric)
EpiQuik HDAC Activity/Inhibition Assay Kit (Colorimetric)
EpiQuik Dnmt1 Assay Kit
EpiQuik Dnmt1 Assay Kit
Epigentek is an epigenetics company that specializes in epigenetic kits, antibodies, reagents, and services for epigenetic research in DNA methylation, histone modification, and chromatin studies. Terms & Conditions | Privacy Policy | Site Map
Copyright © 2017 Epigentek Group Inc. All rights reserved.