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Histone H3R17 Dimethyl Asymmetric (H3R17me2a) Polyclonal Antibody

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Western blot analysis of extracts of various cell lines, using Asymmetric DiMethyl-Histone H3-R17 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Dot-blot analysis of all sorts of methylation peptides using Asymmetric DiMethyl-Histone H3-R17 antibody.
Immunohistochemistry of paraffin-embedded rat lung using Asymmetric DiMethyl-Histone H3-R17 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using Asymmetric DiMethyl-Histone H3-R17 antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using Asymmetric DiMethyl-Histone H3-R17 antibody at dilution of 1:200 (40x lens).
Immunofluorescence analysis of 293T cells using Asymmetric DiMethyl-Histone H3-R17 antibody. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using Asymmetric DiMethyl-Histone H3-R17 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Application: IF, IHC, IP, WB
Areas of Research: Histone Methylation & Demethylation
Clonality: Polyclonal
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
Purification: Affinity Purified
Reactivity: Broad Range, Human, Mouse, Rat
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-3709-05050 µl $253.00 
A-3709-100100 µl $399.00 
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Product Overview

Description
Histone H3R17 Dimethyl Asymmetric (H3R17me2a) Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Liquid. PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Specificity
Human, Mouse, Rat, Broad Range

Isotype
IgG

Uniprot ID
P68431

Purification
Affinity Purified

Immunogen
Synthetic Peptide of Human Asymmetric DiMethyl-Histone H3-R17

Storage
Shipped at 4°C. Store at -20°C. Avoid multiple freeze/thaw cycles.

Alternative Names
H3R17me2a, HIST1H3J, H3/j , H3FJ , Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h , Histone H3/I, Histone H3/j , Histone H3/k, Histone H3/l, H3 Arginine 17 me2a

Application
WB, IHC, IF, IP; Recommended dilution: WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200 IP 1:50 - 1:200

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Safety Data Sheet]
BisulPlus Loci 5mC & 5hmC Detection PCR kit
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