Histone H3R17 Dimethyl Asymmetric (H3R17me2a) Polyclonal Antibody

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Western blot analysis of lysates from HeLa cells using Histone H3R17 Dimethyl Asymmetric (H3R17me2a) Polyclonal Antibody at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 10s.

Dot-blot analysis of all sorts of methylation peptides using Histone H3R17 Dimethyl Asymmetric (H3R17me2a) Polyclonal Antibody.

Immunofluorescence analysis of 293T cells using Histone H3R17 Dimethyl Asymmetric (H3R17me2a) Polyclonal Antibody. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using Histone H3R17 Dimethyl Asymmetric (H3R17me2a) Polyclonal Antibody at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Application:	ELISA, IF/ICC, WB
Areas of Research:	Histone Methylation & Demethylation
Clonality:	Polyclonal
Conjugate:	Unconjugated
Host:	Rabbit
Isotype:	IgG
Purification:	Affinity Purified
Reactivity:	Broad Range, Human, Mouse, Rat
100% Guarantee:	6 months

Catalog No.	Size	Price	Qty
A-3709-050	50 µl	$306.00
A-3709-100	100 µl	$477.00

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Product Overview
Datasheet & SDS
Product FAQ

Product Overview

Description
Histone H3R17 Dimethyl Asymmetric (H3R17me2a) Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Specificity
Human, Mouse, Rat, Broad Range

Isotype
IgG

Uniprot ID
Q16695/P68431

Purification
Affinity Purified

Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human histone H3 (NP_003520.1).

Storage
Shipped at 4°C. Upon receipt, store at -20°C. Avoid repeated freeze.

Alternative Names
H3R17me2a, HIST1H3J, H3/j , H3FJ , Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h , Histone H3/I, Histone H3/j , Histone H3/k, Histone H3/l, H3 Arginine 17 me2a

Application
WB, IF/ICC, ELISA; Recommended dilution: WB 1:500 - 1:1000, IF 1:50 - 1:200, ELISA - recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Datasheet & SDS

Datasheet/User Guide
If the above file is not available online, you can request a copy by contacting us.

[Safety Data Sheet]

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Histone H3R17 Dimethyl Asymmetric (H3R17me2a) Polyclonal Antibody