Primary Antibodies

Epigentek's well characterized and highly validated DNA methylation antibodies and DNA demethylation antibodies may help to increase researchers' understanding of DNA methylation and its involvement in diseases as well as cell cycle regulation and embryonic development. DNA methylation is the most widely studied epigenetic mechanism that regulates gene expression, thereby affecting cell function, while aberrant DNA methylation is prevalent in many diseases, particularly cancer.

Histone modification antibodies are used to investigate epigenetic modifications to histones such as acetylation, methylation, and phosphorylation. Histone methylation is associated with transcriptional repression or activation via the transfer of methyl groups to lysine or arginine residues of histone proteins and is crucial to studying epigenetic gene regulation with the use of histone methylation antibodies. Histone methyltransferases (HMTs) are responsible for catalyzing this proce...

RNA processing and modifications to RNA are crucial to numerous cell functions and give insight into a multitude of biological processes that are influenced by RNA activities, such as RNA interference. RNA interference (RNAi) is a gene-regulating process in which genes are silenced by RNA molecules such as shRNA, siRNA, and miRNA, usually destroying mRNA molecules and lowering protein expression. RNAi and gene silencing antibodies target the proteins involved in the various steps of g...

Antibodies that target proteins associated with chromatin and transcription such as histone deacetylases (HDACs), histone acetyltransferases (HATs), histone methyltransferases (HMTs), and HP1 are important to understanding the epigenetic regulation of chromatin structure and its function. Chromatin is involved in crucial functions of the cell including division, replication, and gene expression.

Cell structure & function antibodies are used for investigating cell structure and function and include those linked to DNA damage & repair, cell cycle, apoptosis, cellular structure, and trafficking. Cell structure and function antibodies can be used to connect structural features of the cell, such as the cytoskeleton, including microtubules and microfilament, to their function. Cell cycle antibodies are targeted against proteins involved in the steps of the cell cycle, including the ...

Signal transduction, or cell signaling, is the process by which various signals are transmitted throughout the cell in a cascading series of extracellular and intracellular molecular events involving lipids, proteins, and ions. Often it involves a signal from a secreted growth factor, hormone or other extracellular signaling molecule with binding to receptors at the cell surface, including receptor tyrosine kinases and G-protein coupled receptors, which then transduce the signal intrac...

Metabolism is the chemical process catalyzed by certain enzymes by which organisms convert various substances to energy in order to sustain life. Metabolism antibodies are crucial for studying glucose, lipid, protein and nucleotide metabolism. It can be broken into two categories: catabolism and anabolism. Cellular respiration, for example, is a catabolic process as it breaks down substances. Building up nucleic acids and proteins, on the other hand, is anabolic. Understanding metaboli...

Immunology and inflammation antibodies include cell marker and receptor antibodies, cytokine antibodies, and others. Epigenetic mechanisms have been closely involved in inflammatory pathways and using antibodies that recognize specific antigens related to the immune system and inflammation offers researchers insight into the potential pathological epigenetic modifications and immunological and inflammatory complications linked to a multitude of diseases.

Antibodies used for various applications, including the recently discovered gene editing and genetic engineering system known as the CRISPR/Cas9 system. The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and Cas9 (CRISPR associated system or CRISPR associated protein 9 nuclease) system delivers RNA-guided nuclease (Cas9) and specific guide RNAs (gRNA) into a cell.