Epigentek's 8-hydroxyguanosine antibody recognizes 8-hydroxyguanosine (8-OHG), a modified base which occurs in DNA as a result of oxidative stress. 8-hydroxy-2'-deoxyguanosine (8-OHdG) has become a sensitive marker of oxidative damage in cellular DNA and has been reported to be excreted in the urine. Levels of 8-OHdG have been shown to increase on exposure to ionizing radiation and tobacco smoke. Epigentek's 8-hydroxyguanosine polyclonal antibody cross reacts completely with 8-OHdG, and does not cross react with other naturally occurring nucleotides.
Serum - liquid
8-Hydroxyguanosine - conjugate
0.1% Sodium Azide
Store at +4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein.
Should this product contain a precipitate we recommend microcentrifugation before use. For maximum recovery of the products, centrifuge the vial prior to opening the cap.
8 hydroxy 2’ deoxyguanosine, 8 hydroxyguanine, 8 hydroxyguanosine, 8 OHG, 8-OHG, 8OG, 8OHdG, 8OHG
Applications & Suggested Dilutions
ELISA: 1/100,000 - 1/250,000 ; Immunohistology - Paraffin: 1/200; Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.