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ChromaFlash Chromatin Extraction Kit


For isolation of intact chromatin from mammalian cells or tissues

Citations (18) | Write a Review
Schematic procedure of chromation isolation with the ChromaFlash™ Chromatin Extraction Kit.
ChIP analysis of RNA polymerase II enriched in GAPDH and MLH1 promoters with chromatin extract prepared from formaldehyde fixed colon cancer cells (2x105) using the ChromaFlash™ Chromatin Extraction Kit.
Input Type: Unisolated Samples
Research Area: Chromatin & Transcription
Target Application: Sample Isolation
Vessel Format: Columns/Tubes
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-2001-100100 extractions $145.00 
Order now & get it by Wednesday, December 11th  
Product Overview

The ChromaFlash™ Chromatin Extraction Kit is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein complex from mammalian cells or tissues in a simple and rapid format. Chromatin prepared by this kit can be used in a variety of chromatin immunoprecipitation methods. It is also the recommended method for obtaining chromatin required by Epigentek’s one-hour ChIP method using the ChromaFlash™ One-Step ChIP Kit. The isolated chromatin can also be used in other chromatin-related applications such as in vitro protein-DNA binding assays and nuclear enzyme assays.

  • Extremely fast procedure: the entire procedure from cell/tissue sample to ready-to-use chromatin is less than 60 minutes. 
  • Convenient and flexible: the kit is suitable for preparing both native chromatin and cross-linked chromatin from monolayer or suspension cells, or from tissues. 
  • Unsheared chromatin makes it customizable for various analysis workflows that require either intact or fragmented chromatin, including ChIP, in vitro protein-DNA interaction analysis, nuclear enzyme assay, etc.

Background Information
Chromatin immunoprecipitaton (ChIP) offers an advantageous tool for studying protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the specific sequences of a gene in living cells by combining with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) techniques. For example, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated with a specific gene promoter region under various conditions can be achieved through a ChIP-PCR assay, while recruitment of meH3-K9 to the promoters on a genome-wide scale can be detected by ChIP-chip. In particular, the ChIP method with specific antibodies directly against various transcriptional factors is widely demanded. 

Principle & Procedure
The ChromaFlash™ Chromatin Extraction Kit contains all reagents required for carrying out successful chromatin extraction directly from mammalian cells or tissues. Cell membranes of the sample, with or without cross-linking, are broken down using the provided lysis buffer. Chromatin or DNA-protein complex is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and stored at the appropriate temperature.

Starting Materials & Input Amount
Starting materials can include various tissue or cell samples such as cells from flask or microplate cultured cells, fresh and frozen tissues, etc. The amount of cells and tissues for each preparation can be 1 x 105 to 5 x 106 cells and 10 mg to 200 mg, respectively. For optimal preparation, the input amount should be 1 to 5 x 106 cells or 50 to 200 mg tissues.  Yield of chromatin is approximately 4 ug per 106  cells or per 50 mg tissues.

User Guide & MSDS

[User Guide]*
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[Safety Data Sheet]
Product Citations

Gomes AP et. al. (September 2019). Dynamic Incorporation of Histone H3 Variants into Chromatin Is Essential for Acquisition of Aggressive Traits and Metastatic Colonization. Cancer Cell.

Burgess S et. al. (August 2019). Gut microbiome communication with bone marrow regulates susceptibility to amebiasis bioRxiv.

Bhargavan B et. al. (July 2019). Epigenetics, N-myrystoyltransferase-1 and casein kinase-2-alpha modulates the increased replication of HIV-1 CRF02_AG, compared to subtype-B viruses. Sci Rep. 9(1):10689.

Behera J et. al. (October 2018). Hydrogen Sulfide Promotes Bone Homeostasis by Balancing Inflammatory Cytokine Signaling in CBS-Deficient Mice through an Epigenetic Mechanism. Sci Rep. 8(1):15226.

Behera J et. al. (June 2018). Hydrogen sulfide epigenetically mitigates bone loss through OPG/RANKL regulation during hyperhomocysteinemia in mice. Bone.

Brocklehurst S et. al. (February 2018). Induction of epigenetic variation in Arabidopsis by over-expression of DNA METHYLTRANSFERASE1 (MET1). PLoS One. 13(2):e0192170.

Zhou S et. al. (August 2017). Intermittent hypoxia-induced cardiomyopathy and its prevention by Nrf2 and metallothionein. Free Radic Biol Med.

Mathison M et. al. (June 2017). Cardiac reprogramming factor Gata4 reduces postinfarct cardiac fibrosis through direct repression of the profibrotic mediator snail. J Thorac Cardiovasc Surg.

Upadhyaya B et. al. (April 2017). Prenatal Exposure to a Maternal High-Fat Diet Affects Histone Modification of Cardiometabolic Genes in Newborn Rats. Nutrients. 9(4)

R. Manoharlal et. al. (March 2017). Gibberellin A3 as an epigenetic determinant of global DNA hypo-methylation in tobacco Biologia Plantarum. :1-13.

Gonzalez-Vasconcellos I et. al. (February 2017). The Rb1 tumour suppressor gene modifies telomeric chromatin architecture by regulating TERRA expression. Sci Rep. 7:42056.

Bhargavan B et. al. (November 2015). Toll-like receptor-3 mediates HIV-1 transactivation via NFκB and JNK pathways and histone acetylation, but prolonged activation suppresses Tat and HIV-1 replication. Cell Signal.

Liu L et. al. (March 2015). Knockdown of SALL4 enhances all-trans retinoic acid-induced cellular differentiation in acute myeloid leukemia cells. J Biol Chem.

Gonzalez-Vasconcellos I et. al. (January 2015). Telomere Chromatin Condensation Assay (TCCA): a novel approach to study structural telomere integrity. Mutat Res. 771:51-5.

Weyler L et. al. (December 2014). Restriction Endonucleases from Invasive Neisseria gonorrhoeae Cause Double-Strand Breaks and Distort Mitosis in Epithelial Cells during Infection. PLoS One. 9(12):e114208.

Ranjan R et. al. (June 2014). The Transcription Factor Nuclear Factor of Activated T Cells c3 Modulates the Function of Macrophages in Sepsis. J Innate Immun.

Liu L et. al. (November 2013). Histone lysine-specific demethylase 1 (LSD1) protein is involved in Sal-like protein 4 (SALL4)-mediated transcriptional repression in hematopoietic stem cells. J Biol Chem. 288(48):34719-28.

Kim KI et. al. (May 2013). Changes in the epigenetic status of the SOX-9 promoter in human osteoarthritic cartilage. J Bone Miner Res. 28(5):1050-60.

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