ChromaFlash Plant Chromatin Extraction Kit

The ChromaFlash™ Plant Chromatin Extraction Kit is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein complexes from plants in a simple and rapid format. Chromatin prepared by this kit can be used in a variety of chromatin immunoprecipitation methods. It is also the recommended method for obtaining chromatin required by Epigentek’s one-hour ChIP method using the ChromaFlash™ One-Step ChIP Kit or the ChromaFlash™ One-Step Magnetic ChIP Kit. The isolated chromatin can also be used in other chromatin-related applications such as in vitro protein-DNA binding assays, or nuclear enzyme assays.

• Extremely fast procedure: the entire procedure from plant samples to ready-to-use chromatin is less than 2 hours.
• Convenient and flexible: the kit is suitable for preparing both native chromatin and cross-linked chromatin from monolayer or suspension cells, or from tissues. 
• Unsheared chromatin makes it customizable for various analysis workflows that require either intact or fragmented chromatin, including ChIP, in vitro protein-DNA interaction analysis, nuclear enzyme assays, etc.

Background Information
Chromatin immunoprecipitation (ChIP) offers an advantageous tool for studying protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the specific sequences of a gene in living cells by combining it with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) techniques. For example, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated with a specific gene promoter region under various conditions can be achieved through a ChIP-PCR assay, while recruitment of meH3-K9 to the promoters on a genome-wide scale can be detected by ChIP-chip. In particular, a ChIP method with specific antibodies directly against various transcriptional factors is widely in demand.