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ChromaFlash Plant Chromatin Extraction Kit


For isolation of intact chromatin from plant cells or tissues

Citations (1) | (1) | Write a Review
Schematic procedure of the ChromaFlash™ Plant Chromatin Extraction Kit.
ChIP analysis of tri-methyl H3-K27 enriched in AGAMOUS gene with chromatin extract prepared from 2-week-old icu2-1/icu2-1 seedlings using the ChromaFlash™ Plant Chromatin Extraction Kit.
Input Type: Unisolated Samples
Research Area: Chromatin & Transcription
Target Application: Sample Isolation
Vessel Format: Columns/Tubes
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-2022-05050 extractions $156.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

The ChromaFlash™ Plant Chromatin Extraction Kit is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein complexes from plants in a simple and rapid format. Chromatin prepared by this kit can be used in a variety of chromatin immunoprecipitation methods. It is also the recommended method for obtaining chromatin required by Epigentek’s one-hour ChIP method using the ChromaFlash™ One-Step ChIP Kit or the ChromaFlash™ One-Step Magnetic ChIP Kit. The isolated chromatin can also be used in other chromatin-related applications such as in vitro protein-DNA binding assays, or nuclear enzyme assays.

  • Extremely fast procedure: the entire procedure from plant samples to ready-to-use chromatin is less than 2 hours.
  • Convenient and flexible: the kit is suitable for preparing both native chromatin and cross-linked chromatin from monolayer or suspension cells, or from tissues. 
  • Unsheared chromatin makes it customizable for various analysis workflows that require either intact or fragmented chromatin, including ChIP, in vitro protein-DNA interaction analysis, nuclear enzyme assays, etc.

Background Information
Chromatin immunoprecipitation (ChIP) offers an advantageous tool for studying protein-DNA interaction. With ChIP, the experimenter can determine if a specific protein binds to the specific sequences of a gene in living cells by combining it with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) techniques. For example, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated with a specific gene promoter region under various conditions can be achieved through a ChIP-PCR assay, while recruitment of meH3-K9 to the promoters on a genome-wide scale can be detected by ChIP-chip. In particular, a ChIP method with specific antibodies directly against various transcriptional factors is widely in demand.

Principle & Procedure
The ChromaFlash™ Plant Chromatin Extraction Kit contains all the reagents required for carrying out a successful chromatin extraction directly from plants. Cell membranes of the sample, with or without cross-linking, are broken down using the provided lysis buffer. Chromatin or DNA-protein complex is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and stored at the appropriate temperature.

Fig. 1. Schematic procedure of the ChromaFlash Plant Chromatin Extraction Kit.

Fig. 2. ChIP analysis of tri-methyl H3-K27 enriched in AGAMOUS gene with chromatin extract prepared from 2-week-old icu2-1/icu2-1 seedlings using the ChromaFlash Plant Chromatin Extraction Kit.

Product Components

5X Lysis Buffer
Extraction Buffer A
Extraction Buffer B
Extraction Buffer C 
Chromatin Buffer
Protease Inhibitor Cocktails (1000X)*
User Guide

* Spin the solution down to the bottom prior to use.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

Product Citations

Brocklehurst S et. al. (February 2018). Induction of epigenetic variation in Arabidopsis by over-expression of DNA METHYLTRANSFERASE1 (MET1). PLoS One. 13(2):e0192170.

Customer Reviews

Rating by l**********@ars.usda.gov Verified Purchase Reviewed on: Thursday 08 December, 2016
Application Description
Chromatin isolation is very easy by using this kit. It worked fine with tomato fruit tissues.
MethylFlash ELISA Easy
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