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EpiQuik Plant ChIP Kit

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For immunoprecipitating chromatin specifically from plant input samples

Citations (48) | (2) | Write a Review
Suggested Workflow
Chromatin Isolation
 
 
Chromatin Shearing
 
 
ChIP
 
 
PCR Analysis
 
Schematic procedure of the EpiQuik™ Plant ChIP Kit.
DNA was immunoprecipitated from 2-week-old icu2-1/icu2-1 seedlings. PCR was used to amplify the ORNITHINE TRANSCARBAMILASE (OTC) gene and regions of the AGAMOUS gene.
Input Type: Chromatin
Research Area: Chromatin & Transcription
Target Application: Immunoprecipitation
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-2014-2424 reactions $299.00 
P-2014-4848 reactions $499.00 
Availability: Usually Ships In 1-2 Days 
Product Overview

The EpiQuik™ Plant ChIP Kit is a convenient package of tools that allows the experimenter to investigate protein-DNA interaction in vivo efficiently. The entire procedure can be completed within 6 hours and produces far superior results than any competitor kits. The EpiQuik™ Plant ChIP Kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, ChIP-Seq, and ChIP-on-chip. This kit has the following advantages:

  • The fastest procedure available, which can be finished within 6 hours.
  • Strip microwell format makes the assay flexible: manual or high throughput.
  • Columns for DNA purification are included: save time and reduce labor.
  • Compatible with all DNA amplification-based approaches.
  • Simple, reliable, and consistent assay conditions.

See also a quick chart to compare ChIP kits.

Background Information
Protein-DNA interaction play a critical role for cellular functions such as signal transduction, gene transcription, chromosome segregation, DNA replication and recombination, and epigenetic silencing. In plants, interactions between the DNA-binding proteins and cognate promoter sequences are primary determinants in establishing spatial and temporal expression patterns of genes that effect homeostasis, development, and adaptation. Chromatin Immunoprecipitation (ChIP) offers an advantageous tool for identifying direct genomewide associations between specific regulatory proteins and their target genes. Unlike other methods such as EMASA, DNA microarrays, and report gene assays, which analyze direct interactions between protein and DNA in vitro, ChIP can detect that a specific protein binds to the specific sequences of a gene in living cells.

Principle & Procedure
This ChIP kit includeds all reagents required for carrying out a successful chromatin immunoprecipitation from plant cells. Particularly, this kit includes a ChIP-grade dimethyl-histone H3-K9 antibody and a negative control normal mouse IgG. Chromatin from the cells is extracted, sheared, and added into the microwell immobilized with the antibody. DNA is released from the antibody-captured protein-DNA complex, reversed and purified through the specifically designed F-Spin Column. Eluted DNA can be used for various down-stream applications.

Starting Materials
Starting materials can include various plant tissue (flowers, leaves, young seedlings). In general, the input amount should be from 20 to 50 mg of plant tissue for each reaction.

Product Components

CP1 (Wash Buffer)
CP2 (Antibody Buffer)
CP3C (5X Lysis Buffer I)
CP3D (Lysis Buffer II)
CP3E (Lysis Buffer III)
CP3F (Lysis Buffer IV)
CP4 (ChIP Dilution Buffer)
CP5 (DNA Release Buffer)
CP6 (Reverse Buffer)
CP7 (Binding Buffer)
CP8 (Elution Buffer)
Protease Inhibitor Cocktail (100X)*
Normal Mouse IgG (1 mg/ml)*
Anti-Dimethyl H3-K9 (1 mg/ml)*
Proteinase K (10 mg/ml)*
8-Well Assay Strips (with Frame)
8-Well Strip Caps
F-Spin Column
F-Collection Tube
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]
Product Citations

Cai SY et. al. (January 2017). HsfA1a upregulates melatonin biosynthesis to confer cadmium tolerance in tomato plants. J Pineal Res.

Yanan He et. al. (December 2016). Phytochrome B Negatively Affects Cold Tolerance by Regulating OsDREB1 Gene Expression through Phytochrome Interacting Factor-Like Protein OsPIL16 in Rice Frontiers in Plant Science.

Liu G et. al. (October 2016). Local Transcriptional Control of YUCCA Regulates Auxin Promoted Root-Growth Inhibition in Response to Aluminium Stress in Arabidopsis. PLoS Genet. 12(10):e1006360.

Mondal S et. al. (August 2016). Characterization of histone modifications associated with DNA damage repair genes upon exposure to gamma rays in Arabidopsis seedlings. J Radiat Res.

Huang YC et. al. (August 2016). The heat-stress factor HSFA6b connects ABA signaling and ABA-mediated heat responses. Plant Physiol.

Li T et. al. (August 2016). Apple (Malus domestica) MdERF2 Negatively Affects Ethylene Biosynthesis During Fruit Ripening by Suppressing MdACS1 Transcription. Plant J.

Yan JY et. al. (May 2016). A WRKY transcription factor regulates Fe translocation under Fe deficiency in Arabidopsi. Plant Physiol.

Bai S et. al. (April 2016). Epigenetic regulation of MdMYB1 is associated with paper bagging-induced red pigmentation of apples. Planta.

Yu Y et. al. (February 2016). Salt stress and ethylene antagonistically regulate nucleocytoplasmic partitioning of COP1 to control seed germination. Plant Physiol.

Zhang S et. al. (December 2015). Two domain-disrupted hda6 alleles have opposite epigenetic effects on transgenes and some endogenous targets. Sci Rep. 5:17832.

Wang Y et. al. (November 2015). Tomato HsfA1a plays a critical role in plant drought tolerance by activating ATG genes and inducing autophagy. Autophagy. 11(11):2033-2047.

Su L et. al. (October 2015). OsHAL3, a new component interacts with the floral regulator Hd1 to activate flowering in rice. Mol Plant.

Ding ZJ et. al. (August 2015). Transcription factor WRKY46 modulates the development of Arabidopsis lateral roots in osmotic/salt stress conditions via regulation of ABA signaling and auxin homeostasis. Plant J.

Gao Q et. al. (August 2015). Overexpression of a novel cold-responsive transcript factor LcFIN1 from sheepgrass enhances tolerance to low temperature stress in transgenic plants. Plant Biotechnol J.

Qiu K et. al. (July 2015). EIN3 and ORE1 Accelerate Degreening during Ethylene-Mediated Leaf Senescence by Directly Activating Chlorophyll Catabolic Genes in Arabidopsis. PLoS Genet. 11(7):e1005399.

Yang W et. al. (June 2015). Intronic promoter-mediated feedback loop regulates bean PvSR2 gene expression. Biochem Biophys Res Commun.

Zhang W et. al. (May 2015). Genome-wide histone acetylation correlates with active transcription in maize. Genomics.

Wang F et. al. (May 2015). GmWRKY27 interacts with GmMYB174 to reduce expression of GmNAC29 for stress tolerance in soybean plants. Plant J.

Yan D et. al. (April 2015). CURVED CHIMERIC PALEA 1 encoding an EMF1-like protein maintains epigenetic repression of OsMADS58 in rice palea development. Plant J. 82(1):12-24.

Sun N et. al. (March 2015). Bean metal-responsive element-binding transcription factor confers cadmium resistance in tobacco. Plant Physiol. 167(3):1136-48.

Majerová E et. al. (November 2014). Chromatin features of plant telomeric sequences at terminal vs. internal positions. Front Plant Sci. 5:593.

Saito T et. al. (October 2014). Histone modification and signaling cascade of the dormancy-associated MADS-box gene, PpMADS13-1, in Japanese pear (Pyrus pylifolia) during endodormancy. Plant Cell Environ.

Han M et. al. (July 2014). OsWRKY42 Represses OsMT1d and Induces Reactive Oxygen Species and Leaf Senescence in Rice. Mol Cells. 37(7):532-9.

Li C et. al. (July 2014). An ABA-responsive DRE-binding protein gene from Setaria italica, SiARDP, the target gene of SiAREB, plays a critical role under drought stress. J Exp Bot.

Ding ZJ et. al. (June 2014). WRKY41 controls Arabidopsis seed dormancy via direct regulation of ABI3 transcript levels not downstream of ABA. Plant J.

Luna E et. al. (May 2014). Role of NPR1 and KYP in long-lasting induced resistance by β-aminobutyric acid. Front Plant Sci. 5:184.

Lu D et. al. (May 2014). Transcriptional control of ROS homeostasis by KUODA1 regulates cell expansion during leaf development. Nat Commun. 5:3767.

Ding ZJ et. al. (April 2014). Transcription factor WRKY46 regulates osmotic stress responses and stomatal movement independently in Arabidopsis. Plant J.

Schmidt R et. al. (February 2014). SALT-RESPONSIVE ERF1 is a negative regulator of grain filling and gibberellin-mediated seedling establishment in rice. Mol Plant. 7(2):404-21.

Ding ZJ et. al. (December 2013). WRKY46 functions as a transcriptional repressor of ALMT1, regulating aluminum-induced malate secretion in Arabidopsis. Plant J. 76(5):825-35.

Schmidt R et. al. (October 2013). MULTIPASS, a rice R2R3-type MYB transcription factor, regulates adaptive growth by integrating multiple hormonal pathways. Plant J. 76(2):258-73.

Liu X et. al. (September 2013). Auxin controls seed dormancy through stimulation of abscisic acid signaling by inducing ARF-mediated ABI3 activation in Arabidopsis. Proc Natl Acad Sci U S A. 110(38):15485-90.

Křížová K et. al. (June 2013). Epigenetic switches of tobacco transgenes associate with transient redistribution of histone marks in callus culture. Epigenetics. 8(6):666-76.

Schmidt R et. al. (June 2013). Salt-responsive ERF1 regulates reactive oxygen species-dependent signaling during the initial response to salt stress in rice. Plant Cell. 25(6):2115-31.

Liu J et. al. (May 2013). An autoregulatory loop controlling Arabidopsis HsfA2 expression: role of heat shock-induced alternative splicing. Plant Physiol. 162(1):512-21.

Makarevitch I et. al. (March 2013). Genomic distribution of maize facultative heterochromatin marked by trimethylation of H3K27. Plant Cell. 25(3):780-93.

Eichten SR et. al. (December 2012). Spreading of heterochromatin is limited to specific families of maize retrotransposons. PLoS Genet. 8(12):e1003127.

Dalakouras A et. al. (September 2012). Transgenerational maintenance of transgene body CG but not CHG and CHH methylation. Epigenetics. 7(9):1071-8.

Ogrocká A et. al. (June 2012). Developmental silencing of the AtTERT gene is associated with increased H3K27me3 loading and maintenance of its euchromatic environment. J Exp Bot. 63(11):4233-41.

Yang X et. al. (May 2012). Evolution of double positive autoregulatory feedback loops in CYCLOIDEA2 clade genes is associated with the origin of floral zygomorphy. Plant Cell. 24(5):1834-47.

Luna E et. al. (February 2012). Next-generation systemic acquired resistance. Plant Physiol. 158(2):844-53.

Kim JS et. al. (December 2011). An ABRE promoter sequence is involved in osmotic stress-responsive expression of the DREB2A gene, which encodes a transcription factor regulating drought-inducible genes in Arabidopsis. Plant Cell Physiol. 52(12):2136-46.

Yamaguchi T et. al. (August 2010). Cortisol is involved in temperature-dependent sex determination in the Japanese flounder. Endocrinology. 151(8):3900-8.

Hirai S et. al. (April 2010). Loss of sense transgene-induced post-transcriptional gene silencing by sequential introduction of the same transgene sequences in tobacco. FEBS J. 277(7):1695-703.

Guo Z et. al. (April 2010). TCP1 modulates brassinosteroid biosynthesis by regulating the expression of the key biosynthetic gene DWARF4 in Arabidopsis thaliana. Plant Cell. 22(4):1161-73.

Shibuya K et. al. (February 2009). RNA-directed DNA methylation induces transcriptional activation in plants. Proc Natl Acad Sci U S A. 106(5):1660-5.

Aoyama T et. al. (January 2008). Cell-specific epigenetic regulation of ChM-I gene expression: crosstalk between DNA methylation and histone acetylation. Biochem Biophys Res Commun. 365(1):124-30.

Barrero JM et. al. (September 2007). INCURVATA2 encodes the catalytic subunit of DNA Polymerase alpha and interacts with genes involved in chromatin-mediated cellular memory in Arabidopsis thaliana. Plant Cell. 19(9):2822-38.

Customer Reviews

Rating By l**********@ars.usda.gov Verified Purchase Reviewed on: Thursday 08 December, 2016
Application Description
This kit works fine for plant polyclonal antibodies. We tried it for tomato and it worked fantastic.
Rating By r*****@iastate.edu Verified Purchase Reviewed on: Wednesday 04 February, 2015
Application Description
I would rate this product as a 4. While most of the problems I have had I think stem from using a difficult species model. The ease of use is a 5. It is extremely easy to use and fast in comparison to other established methods.

Other Thoughts
I was thinking about one minor issue with the kit that I thought might be helpful to others that might want to buy it. There really should be some note in the kit specifying which reagents are limiting for the number of tissues and such. Like, will one kit be sufficient to look at 6 tissues, or will I run out of CP3C.
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