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EpiQuik Plant ChIP Kit


For immunoprecipitating chromatin specifically from plant input samples

Citations (71) | (2) | Write a Review
Suggested Workflow
Chromatin Isolation
Chromatin Shearing
PCR Analysis
Schematic procedure of the EpiQuik™ Plant ChIP Kit.
DNA was immunoprecipitated from 2-week-old icu2-1/icu2-1 seedlings. PCR was used to amplify the ORNITHINE TRANSCARBAMILASE (OTC) gene and regions of the AGAMOUS gene.
Input Type: Chromatin
Research Area: Chromatin & Transcription
Target Application: Immunoprecipitation
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-2014-2424 reactions $308.00 
P-2014-4848 reactions $509.00 
Order now & get it by Tuesday, November 26th  
Product Overview

The EpiQuik™ Plant ChIP Kit is a convenient package of tools that allows the experimenter to investigate protein-DNA interaction in vivo efficiently. The entire procedure can be completed within 6 hours and produces far superior results than any competitor kits. The EpiQuik™ Plant ChIP Kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, ChIP-Seq, and ChIP-on-chip. This kit has the following advantages:

  • The fastest procedure available, which can be finished within 6 hours.
  • Strip microwell format makes the assay flexible: manual or high throughput.
  • Columns for DNA purification are included: save time and reduce labor.
  • Compatible with all DNA amplification-based approaches.
  • Simple, reliable, and consistent assay conditions.

See also a quick chart to compare ChIP kits.

Background Information
Protein-DNA interaction play a critical role for cellular functions such as signal transduction, gene transcription, chromosome segregation, DNA replication and recombination, and epigenetic silencing. In plants, interactions between the DNA-binding proteins and cognate promoter sequences are primary determinants in establishing spatial and temporal expression patterns of genes that effect homeostasis, development, and adaptation. Chromatin Immunoprecipitation (ChIP) offers an advantageous tool for identifying direct genomewide associations between specific regulatory proteins and their target genes. Unlike other methods such as EMASA, DNA microarrays, and report gene assays, which analyze direct interactions between protein and DNA in vitro, ChIP can detect that a specific protein binds to the specific sequences of a gene in living cells.

Principle & Procedure
This ChIP kit includeds all reagents required for carrying out a successful chromatin immunoprecipitation from plant cells. Particularly, this kit includes a ChIP-grade dimethyl-histone H3-K9 antibody and a negative control normal mouse IgG. Chromatin from the cells is extracted, sheared, and added into the microwell immobilized with the antibody. DNA is released from the antibody-captured protein-DNA complex, reversed and purified through the specifically designed F-Spin Column. Eluted DNA can be used for various down-stream applications.

Starting Materials
Starting materials can include various plant tissue (flowers, leaves, young seedlings). In general, the input amount should be from 20 to 50 mg of plant tissue for each reaction.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Safety Data Sheet]
Product Citations

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Han Q et. al. (October 2019). ZmDREB1A regulates RAFFINOSE SYNTHASE controlling raffinose accumulation and plant chilling stress tolerance in maize. Plant Cell Physiol.

Wang F et. al. (October 2019). Crosstalk of PIF4 and DELLA modulates CBF transcript and hormone homeostasis in cold response in tomato. Plant Biotechnol J.

Hou J et al. et. al. (September 2019). Glutaredoxin S25 and its interacting TGACG motif-binding factor TGA2 mediate brassinosteroid-induced chlorothalonil metabolism in tomato plants Environmental Pollution.

Song N et. al. (July 2019). An ERF2-like transcription factor regulates production of the defense sesquiterpene capsidiol upon Alternaria alternata infection. J Exp Bot.

Nguyen NH et. al. (June 2019). Chromatin remodeling for the transcription of type 2C protein phosphatase genes in response to salt stress. Plant Physiol Biochem. 141:325-331.

Liu D et. al. (May 2019). An R1R2R3 MYB Transcription Factor, MnMYB3R1, Regulates the Polyphenol Oxidase Gene in Mulberry (<i>Morus notabilis</i>). Int J Mol Sci. 20(10)

Sato H et. al. (May 2019). NF-YB2 and NF-YB3 have functionally diverged and differentially induce drought and heat stress-specific genes. Plant Physiol.

Na Song et. al. (March 2019). Capsidiol, a defensive sesquiterpene produced by wild tobacco in response to attack from the fungal pathogen Alternaria alternata, is regulated by an ERF2-like transcription factor biorxiv.

M. Salehin et. al. (March 2019). Auxin-sensitive Aux/IAA proteins mediate drought tolerance in Arabidopsis by regulating glucosinolate levels biorxiv.

Nguyen NH et. al. (March 2019). AtMYB44 suppresses transcription of the late embryogenesis abundant protein gene AtLEA4-5. Biochem Biophys Res Commun.

Dai Z et. al. (February 2019). The OsmiR396-OsGRF8-OsF3H-flavonoid pathway mediates resistance to the brown planthopper in rice (Oryza sativa). Plant Biotechnol J.

Wang F et. al. (December 2018). SlHY5 Integrates Temperature, Light and Hormone Signaling to Balance Plant Growth and Cold Tolerance. Plant Physiol.

Ryu TH et. al. (December 2018). SOG1-dependent NAC103 modulates the DNA damage response as a transcriptional regulator in Arabidopsis. Plant J.

Nguyen NH et. al. (December 2018). AtMYB44 interacts with TOPLESS-RELATED corepressors to suppress protein phosphatase 2C gene transcription. Biochem Biophys Res Commun. 507(1-4):437-442.

Zhao P et. al. (September 2018). LcbHLH92 from sheepgrass acts as a negative regulator of anthocyanin/proanthocyandin accumulation and influences seed dormancy. J Exp Bot.

Frank A et. al. (July 2018). Circadian Entrainment in Arabidopsis by the Sugar-Responsive Transcription Factor bZIP63. Curr Biol.

Nguyen NH et. al. (June 2018). The AtMYB44 promoter is accessible to signals that induce different chromatin modifications for gene transcription. Plant Physiol Biochem. 130:14-19.

Kong X et. al. (June 2018). Ethylene Promotes Cadmium-induced Root Growth Inhibition through EIN3 controlled XTH33 and LSU1 expression in Arabidopsis. Plant Cell Environ.

Nguyen NH et. al. (April 2018). H2A.Z-containing nucleosomes are evicted to activate AtMYB44 transcription in response to salt stress. Biochem Biophys Res Commun.

Liu CC et. al. (March 2018). The bZip transscription factor HY5 mediates CRY1a-induced anthocyanin biosynthesis in tomato. Plant Cell Environ.

Brocklehurst S et. al. (February 2018). Induction of epigenetic variation in Arabidopsis by over-expression of DNA METHYLTRANSFERASE1 (MET1). PLoS One. 13(2):e0192170.

Uji Y et. al. (June 2017). Identification of OsMYC2-regulated senescence-associated genes in rice. Planta. 245(6):1241-1246.

Cai SY et. al. (January 2017). HsfA1a upregulates melatonin biosynthesis to confer cadmium tolerance in tomato plants. J Pineal Res.

Yanan He et. al. (December 2016). Phytochrome B Negatively Affects Cold Tolerance by Regulating OsDREB1 Gene Expression through Phytochrome Interacting Factor-Like Protein OsPIL16 in Rice Frontiers in Plant Science.

Liu G et. al. (October 2016). Local Transcriptional Control of YUCCA Regulates Auxin Promoted Root-Growth Inhibition in Response to Aluminium Stress in Arabidopsis. PLoS Genet. 12(10):e1006360.

Mondal S et. al. (August 2016). Characterization of histone modifications associated with DNA damage repair genes upon exposure to gamma rays in Arabidopsis seedlings. J Radiat Res.

Huang YC et. al. (August 2016). The heat-stress factor HSFA6b connects ABA signaling and ABA-mediated heat responses. Plant Physiol.

Li T et. al. (August 2016). Apple (Malus domestica) MdERF2 Negatively Affects Ethylene Biosynthesis During Fruit Ripening by Suppressing MdACS1 Transcription. Plant J.

Yan JY et. al. (May 2016). A WRKY transcription factor regulates Fe translocation under Fe deficiency in Arabidopsi. Plant Physiol.

Bai S et. al. (April 2016). Epigenetic regulation of MdMYB1 is associated with paper bagging-induced red pigmentation of apples. Planta.

Yu Y et. al. (February 2016). Salt stress and ethylene antagonistically regulate nucleocytoplasmic partitioning of COP1 to control seed germination. Plant Physiol.

Zhang S et. al. (December 2015). Two domain-disrupted hda6 alleles have opposite epigenetic effects on transgenes and some endogenous targets. Sci Rep. 5:17832.

Wang Y et. al. (November 2015). Tomato HsfA1a plays a critical role in plant drought tolerance by activating ATG genes and inducing autophagy. Autophagy. 11(11):2033-2047.

Su L et. al. (October 2015). OsHAL3, a new component interacts with the floral regulator Hd1 to activate flowering in rice. Mol Plant.

Ding ZJ et. al. (August 2015). Transcription factor WRKY46 modulates the development of Arabidopsis lateral roots in osmotic/salt stress conditions via regulation of ABA signaling and auxin homeostasis. Plant J.

Gao Q et. al. (August 2015). Overexpression of a novel cold-responsive transcript factor LcFIN1 from sheepgrass enhances tolerance to low temperature stress in transgenic plants. Plant Biotechnol J.

Qiu K et. al. (July 2015). EIN3 and ORE1 Accelerate Degreening during Ethylene-Mediated Leaf Senescence by Directly Activating Chlorophyll Catabolic Genes in Arabidopsis. PLoS Genet. 11(7):e1005399.

Yang W et. al. (June 2015). Intronic promoter-mediated feedback loop regulates bean PvSR2 gene expression. Biochem Biophys Res Commun.

Zhang W et. al. (May 2015). Genome-wide histone acetylation correlates with active transcription in maize. Genomics.

Wang F et. al. (May 2015). GmWRKY27 interacts with GmMYB174 to reduce expression of GmNAC29 for stress tolerance in soybean plants. Plant J.

Yan D et. al. (April 2015). CURVED CHIMERIC PALEA 1 encoding an EMF1-like protein maintains epigenetic repression of OsMADS58 in rice palea development. Plant J. 82(1):12-24.

Sun N et. al. (March 2015). Bean metal-responsive element-binding transcription factor confers cadmium resistance in tobacco. Plant Physiol. 167(3):1136-48.

Majerová E et. al. (November 2014). Chromatin features of plant telomeric sequences at terminal vs. internal positions. Front Plant Sci. 5:593.

Saito T et. al. (October 2014). Histone modification and signaling cascade of the dormancy-associated MADS-box gene, PpMADS13-1, in Japanese pear (Pyrus pylifolia) during endodormancy. Plant Cell Environ.

Han M et. al. (July 2014). OsWRKY42 Represses OsMT1d and Induces Reactive Oxygen Species and Leaf Senescence in Rice. Mol Cells. 37(7):532-9.

Li C et. al. (July 2014). An ABA-responsive DRE-binding protein gene from Setaria italica, SiARDP, the target gene of SiAREB, plays a critical role under drought stress. J Exp Bot.

Ding ZJ et. al. (June 2014). WRKY41 controls Arabidopsis seed dormancy via direct regulation of ABI3 transcript levels not downstream of ABA. Plant J.

Luna E et. al. (May 2014). Role of NPR1 and KYP in long-lasting induced resistance by β-aminobutyric acid. Front Plant Sci. 5:184.

Lu D et. al. (May 2014). Transcriptional control of ROS homeostasis by KUODA1 regulates cell expansion during leaf development. Nat Commun. 5:3767.

Ding ZJ et. al. (April 2014). Transcription factor WRKY46 regulates osmotic stress responses and stomatal movement independently in Arabidopsis. Plant J.

Schmidt R et. al. (February 2014). SALT-RESPONSIVE ERF1 is a negative regulator of grain filling and gibberellin-mediated seedling establishment in rice. Mol Plant. 7(2):404-21.

Ding ZJ et. al. (December 2013). WRKY46 functions as a transcriptional repressor of ALMT1, regulating aluminum-induced malate secretion in Arabidopsis. Plant J. 76(5):825-35.

Schmidt R et. al. (October 2013). MULTIPASS, a rice R2R3-type MYB transcription factor, regulates adaptive growth by integrating multiple hormonal pathways. Plant J. 76(2):258-73.

Liu X et. al. (September 2013). Auxin controls seed dormancy through stimulation of abscisic acid signaling by inducing ARF-mediated ABI3 activation in Arabidopsis. Proc Natl Acad Sci U S A. 110(38):15485-90.

Křížová K et. al. (June 2013). Epigenetic switches of tobacco transgenes associate with transient redistribution of histone marks in callus culture. Epigenetics. 8(6):666-76.

Schmidt R et. al. (June 2013). Salt-responsive ERF1 regulates reactive oxygen species-dependent signaling during the initial response to salt stress in rice. Plant Cell. 25(6):2115-31.

Liu J et. al. (May 2013). An autoregulatory loop controlling Arabidopsis HsfA2 expression: role of heat shock-induced alternative splicing. Plant Physiol. 162(1):512-21.

Makarevitch I et. al. (March 2013). Genomic distribution of maize facultative heterochromatin marked by trimethylation of H3K27. Plant Cell. 25(3):780-93.

Eichten SR et. al. (December 2012). Spreading of heterochromatin is limited to specific families of maize retrotransposons. PLoS Genet. 8(12):e1003127.

Dalakouras A et. al. (September 2012). Transgenerational maintenance of transgene body CG but not CHG and CHH methylation. Epigenetics. 7(9):1071-8.

Ogrocká A et. al. (June 2012). Developmental silencing of the AtTERT gene is associated with increased H3K27me3 loading and maintenance of its euchromatic environment. J Exp Bot. 63(11):4233-41.

Yang X et. al. (May 2012). Evolution of double positive autoregulatory feedback loops in CYCLOIDEA2 clade genes is associated with the origin of floral zygomorphy. Plant Cell. 24(5):1834-47.

Luna E et. al. (February 2012). Next-generation systemic acquired resistance. Plant Physiol. 158(2):844-53.

Kim JS et. al. (December 2011). An ABRE promoter sequence is involved in osmotic stress-responsive expression of the DREB2A gene, which encodes a transcription factor regulating drought-inducible genes in Arabidopsis. Plant Cell Physiol. 52(12):2136-46.

Yamaguchi T et. al. (August 2010). Cortisol is involved in temperature-dependent sex determination in the Japanese flounder. Endocrinology. 151(8):3900-8.

Hirai S et. al. (April 2010). Loss of sense transgene-induced post-transcriptional gene silencing by sequential introduction of the same transgene sequences in tobacco. FEBS J. 277(7):1695-703.

Guo Z et. al. (April 2010). TCP1 modulates brassinosteroid biosynthesis by regulating the expression of the key biosynthetic gene DWARF4 in Arabidopsis thaliana. Plant Cell. 22(4):1161-73.

Shibuya K et. al. (February 2009). RNA-directed DNA methylation induces transcriptional activation in plants. Proc Natl Acad Sci U S A. 106(5):1660-5.

Aoyama T et. al. (January 2008). Cell-specific epigenetic regulation of ChM-I gene expression: crosstalk between DNA methylation and histone acetylation. Biochem Biophys Res Commun. 365(1):124-30.

Barrero JM et. al. (September 2007). INCURVATA2 encodes the catalytic subunit of DNA Polymerase alpha and interacts with genes involved in chromatin-mediated cellular memory in Arabidopsis thaliana. Plant Cell. 19(9):2822-38.

Customer Reviews

Rating by l**********@ars.usda.gov Verified Purchase Reviewed on: Thursday 08 December, 2016
Application Description
This kit works fine for plant polyclonal antibodies. We tried it for tomato and it worked fantastic.
Rating by r*****@iastate.edu Verified Purchase Reviewed on: Wednesday 04 February, 2015
Application Description
I would rate this product as a 4. While most of the problems I have had I think stem from using a difficult species model. The ease of use is a 5. It is extremely easy to use and fast in comparison to other established methods.

Other Thoughts
I was thinking about one minor issue with the kit that I thought might be helpful to others that might want to buy it. There really should be some note in the kit specifying which reagents are limiting for the number of tissues and such. Like, will one kit be sufficient to look at 6 tissues, or will I run out of CP3C.
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