Epigentek Home
EpiGentek Guarantee

EpiQuik Chromatin Immunoprecipitation (ChIP) Kit


For immunoprecipitating chromatin specifically from cell input samples via microplate format

Citations (170) | (1) | Write a Review
Suggested Workflow
Chromatin Isolation
Chromatin Shearing
PCR Analysis
Schematic procedure of the EpiQuik™ Chromatin Immunoprecipitation (ChIP) Kit.
ChIP was performed according to the protocol of the EpiQuik™ Chromatin Immunoprecipitation (ChIP) Kit using U2OS cells (1 x 106/well).
Comparative overview of the EpiQuik™ Chromatin Immunoprecipitation (ChIP) Kit.
Input Type: Chromatin
Research Area: Chromatin & Transcription
Target Application: Immunoprecipitation
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-2002-124 reactions $249.00 
P-2002-248 reactions $399.00 
P-2002-396 reactions $589.00 
Order now & get it by Wednesday, November 20th  
Product Overview

The EpiQuik™ Chromatin Immunoprecipitation (ChIP) Kit is a complete set of optimized reagents to perform chromatin immunoprecipitation (ChIP) via a convenient, microplate-based format. The kit is ready-to-use and provides all the essential components needed to carry out a successful ChIP experiment. The EpiQuik™ ChIP kits are suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, ChIP-Seq, and ChIP-on-chip. This kit has the following advantages:

  • Fast and easy microplate-based procedure, that can be completed within 5 hours.
  • Strip microwell format makes the assay flexible: manual or high throughput.
  • Columns for DNA purification are included: save tremendous amounts of time and reduce unnecessary physical labor.
  • Compatible with all DNA amplification-based approaches.
  • Achieves very reliable and consistent assay conditions.

See also a quick chart to compare ChIP kits.

Background Information
Protein-DNA interaction plays a critical role in cellular functions such as signal transduction, gene transcription, chromosome segregation, DNA replication and recombination, and epigenetic silencing. Identifying the genetic targets of DNA binding proteins and knowing the mechanisms of protein-DNA interaction is important for understanding the cellular process. Chromatin Immunoprecipitation (ChIP) offers an advantageous tool for studying protein-DNA interactions. Unlike other methods such as EMASA, DNA microarrays, and report gene assays which analyze direct interactions between protein and DNA in vitro- ChIP can detect that a specific protein binds to the specific sequences of a gene in living cells. 

Principle & Procedure
This ChIP kit includes a positive control antibody (RNA polymerase II), a negative control normal mouse IgG, and GAPDH primers that can be used as a positive control to demonstrate the efficacy of the kit reagents and protocol. RNA polymerase II is considered to be enriched in the GAPDH gene promoter that is expected to be undergoing transcription in most growing mammalian cells and can be immunoprecipitated by RNA polymerase II but not by normal mouse IgG. In this ChIP, cells are cross-linked with formaldehyde and chromatin is extracted. The chromatin is then sheared and added into the microwell immobilized with affinity antibodies. Cross-linked DNA is released from antibody-captured protein-DNA complex, reversed and purified through the specifically designed F-Spin Column. Eluted DNA can be used for various downstream applications.

Starting Materials
Starting materials can include various cell samples. In general, the input amount should be from 500,000 to 2,000,000 cells for each reaction.

Frequently Asked Q's

1. Can IP be overnight?

2. Is there any background DNA by mouse IgG IP?
Yes, a little < 2 ng.

3. Can any species Ab can be used for the IP?
Yes, if it is an IgG antibody.

4. Can kit P-2002 be used for frozen tissues?
Yes, but it is not as good as fresh samples.

5. How much volume of elution should be added into PCR reaction?
2 µl.

6. Are cell extracts containing 1%SDS compatible with CP4?
No, the SDS concentration in IP solution should be less than 0.1%.

7. Why is there no difference of CT values between the negative control and samples?
A. Increase washing time by one.
B. Check if antibody is chip grade.
C. There is no enrichment on the promoter.

8. Why is the difference between negative control and positive control not significant?
A. For conventional PCR, reduce PCR cycles to 35-36 or optimize the PCR cycles.
B. Increase washing time by one additional wash.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Safety Data Sheet]
Product Citations

Hosseini M et. al. (September 2019). UVB-induced DHODH upregulation, which is driven by STAT3, is a promising target for chemoprevention and combination therapy of photocarcinogenesis. Oncogenesis. 8(10):52.

Bai R et. al. (September 2019). The NF-κB-modulated miR-19a-3p enhances malignancy of human ovarian cancer cells through inhibition of IGFBP-3 expression. Mol Carcinog.

Zhen Z et. al. (September 2019). Transcription factor E2F4 is a positive regulator of milk biosynthesis and proliferation of bovine mammary epithelial cells. Cell Biol Int.

Kim BG et. al. (August 2019). Compression-induced expression of glycolysis genes in CAFs correlates with EMT and angiogenesis gene expression in breast cancer. Commun Biol. 2:313.

He H et. al. (August 2019). MiR-4429 prevented gastric cancer progression through targeting METTL3 to inhibit m<sup>6</sup>A-caused stabilization of SEC62. Biochem Biophys Res Commun.

He X et. al. (July 2019). Long noncoding RNA GAS5 induces abdominal aortic aneurysm formation by promoting smooth muscle apoptosis. Theranostics. 9(19):5558-5576.

Nazir SU et. al. (June 2019). Breast cancer invasion and progression by MMP-9 through Ets-1 transcription factor. Gene. 711:143952.

Yin Y et. al. (May 2019). The DNA endonuclease Mus81 regulates ZEB1 expression and serves as a target of BET4 inhibitors in gastric cancer. Mol Cancer Ther.

Xie JJ et. al. (May 2019). SP1-mediated overexpression of lncRNA LINC01234 as a ceRNA facilitates non-small-cell lung cancer progression via regulating OTUB1. J Cell Physiol.

Fu Y et. al. (April 2019). Bortezomib-inducible long non-coding RNA myocardial infarction associated transcript is an oncogene in multiple myeloma that suppresses miR-29b. Cell Death Dis. 10(4):319.

Song YS et. al. (April 2019). Interaction of BRAF-induced ETS factors with mutant TERT promoter in papillary thyroid cancer. Endocr Relat Cancer.

Takashina Y et. al. (March 2019). Cyanidin Increases the Expression of Mg<sup>2+</sup> Transport Carriers Mediated by the Activation of PPARα in Colonic Epithelial MCE301 Cells. Nutrients. 11(3)

Hwang HS et. al. (January 2019). Fibronectin fragment inhibits xylosyltransferase-1 expression by regulating Sp1/Sp3-dependent transcription in articular chondrocytes. Osteoarthritis Cartilage.

Manabe A et. al. (November 2018). Upregulation of transient receptor potential melastatin 6 channel expression by rosiglitazone and all-trans-retinoic acid in erlotinib-treated renal tubular epithelial cells. J Cell Physiol.

Chi S et. al. (November 2018). Knockdown of long non-coding HOTAIR enhances the sensitivity to progesterone in endometrial cancer by epigenetic regulation of progesterone receptor isoform B. Cancer Chemother Pharmacol.

Aubrecht TG et. al. (November 2018). Comparing effects of CDK inhibition and E2F1/2 ablation on neuronal cell death pathways in vitro and after traumatic brain injury. Cell Death Dis. 9(11):1121.

Jiang Y et. al. (October 2018). Oncogenic HBXIP enhances ZEB1 through Sp1 to accelerate breast cancer growth. Thorac Cancer.

Takashina Y et. al. (September 2018). Sodium Citrate Increases Expression and Flux of Mg<sup>2+</sup> Transport Carriers Mediated by Activation of MEK/ERK/c-Fos Pathway in Renal Tubular Epithelial Cells. Nutrients. 10(10)

Huang C et. al. (September 2018). The effects of ultrasound exposure on P-glycoprotein-mediated multidrug resistance in vitro and in vivo. J Exp Clin Cancer Res. 37(1):232.

De Souza RAG et. al. (August 2018). Computational Analysis of Transcriptional Regulation Sites at the HTT Gene Locus. J Huntingtons Dis. 7(3):223-237.

Takesue H et. al. (June 2018). Nucleosome positioning and gene regulation of the SGLT2 gene in the renal proximal tubular epithelial cells. Mol Pharmacol.

Yueran Li et. al. (June 2018). The Protective Role of Brain CYP2J in Parkinson’s Disease Models Oxidative Medicine and Cellular Longevity. 2018

Zhang X et. al. (April 2018). Sp1-regulated transcription of RasGRP1 promotes HCC proliferation. Liver Int.

You H et. al. (March 2018). HBX protein promotes LASP-1 expression through activation of c-Jun in human hepatoma cells. J Cell Physiol.

Prasad H et. al. (March 2018). Histone deacetylase-mediated regulation of endolysosomal pH. J Biol Chem.

Zhang F et. al. (March 2018). The involvement of PPARs in the selective regulation of brain CYP2D by growth hormone. Neuroscience.

Li H et. al. (February 2018). Brg1 promotes liver fibrosis via activation of hepatic stellate cells. Exp Cell Res.

Saigusa R et. al. (February 2018). Fli1-haploinsufficient dermal fibroblasts promote skin-localized transdifferentiation of Th2-like regulatory T cells. Arthritis Res Ther. 20(1):23.

Li H et. al. (February 2018). Brg1 promotes liver fibrosis via activation of hepatic stellate cells. Exp Cell Res.

Saigusa R et. al. (February 2018). Fli1-haploinsufficient dermal fibroblasts promote skin-localized transdifferentiation of Th2-like regulatory T cells. Arthritis Res Ther. 20(1):23.

Schartl M et. al. (January 2018). Sox5 is involved in germ-cell regulation and sex determination in medaka following co-option of nested transposable elements. BMC Biol. 16(1):16.

Joung H et. al. (January 2018). Sumoylation of histone deacetylase 1 regulates MyoD signaling during myogenesis. Exp Mol Med. 50(1):e427.

Ma X et. al. (November 2017). Diallyl disulphide inhibits apolipoprotein(a) expression in HepG2 cells through the MEK1-ERK1/2-ELK-1 pathway. Lipids Health Dis. 16(1):223.

Hu J et. al. (November 2017). Sox5 contributes to prostate cancer metastasis and is a master regulator of TGF-β-induced epithelial mesenchymal transition through controlling Twist1 expression. Br J Cancer.

Hu J et. al. (November 2017). Sox5 contributes to prostate cancer metastasis and is a master regulator of TGF-β-induced epithelial mesenchymal transition through controlling Twist1 expression. Br J Cancer.

Lv Y et. al. (October 2017). Glucocorticoids suppressing the browning of adipose tissue via miR-19b in male mice. Endocrinology.

Taniguchi T et. al. (October 2017). A possible implication of reduced levels of LIF, LIFR, and gp130 in vasculopathy related to systemic sclerosis. Arch Dermatol Res.

Takiuchi Y et. al. (October 2017). HTLV-1 bZIP factor suppresses TDP1 expression through inhibition of NRF-1 in adult T-cell leukemia. Sci Rep. 7(1):12849.

Chen S et. al. (September 2017). Sulfur dioxide<sub> </sub>suppresses inflammatory response by sulfenylating NF-κB p65 at cysteine 38 in a rat model of acute lung injury. Clin Sci (Lond).

Lan J et. al. (September 2017). BRG1 promotes VEGF-A expression and angiogenesis in human colorectal cancer cells. Exp Cell Res.

Xu Z et. al. (August 2017). Coenzyme Q10 Improves Lipid Metabolism and Ameliorates Obesity by Regulating CaMKII-Mediated PDE4 Inhibition. Sci Rep. 7(1):8253.

Taniguchi T et. al. (May 2017). Fli1 Deficiency Induces CXCL6 Expression in Dermal Fibroblasts and Endothelial Cells, Contributing to the Development of Fibrosis and Vasculopathy in Systemic Sclerosis. J Rheumatol.

Skapenko A et. al. (May 2017). Defining Epigenetic Regulation of the Interleukin-9 Gene by Chromatin Immunoprecipitation. Methods Mol Biol. 1585:179-187.

Na S et. al. (April 2017). The induction of cytochrome P450 2E1 by ethanol leads to the loss of synaptic proteins via PPARα down-regulation. Toxicology.

Deng X et. al. (March 2017). Linc00152 promotes cancer progression in hepatitis B virus-associated hepatocellular carcinoma. Biomed Pharmacother. 90:100-108.

Kim BG et. al. (March 2017). Mechanical compression induces VEGFA overexpression in breast cancer via DNMT3A-dependent miR-9 downregulation. Cell Death Dis. 8(3):e2646.

Takahashi T et. al. (February 2017). Epithelial Fli1 deficiency drives systemic autoimmunity and fibrosis: Possible roles in scleroderma. J Exp Med.

Qu K et. al. (February 2017). Vitamin C down-regulate apo(a) expression via Tet2-dependent DNA demethylation in HepG2 cells. Int J Biol Macromol.

Huang X et. al. (February 2017). Nuclear Factor of κB1 Is a Key Regulator for the Transcriptional Activation of Milk Synthesis in Bovine Mammary Epithelial Cells. DNA Cell Biol.

Zhang R et. al. (December 2016). Upregulation of microRNA-574-3p in a human gastric cancer cell line AGS by TGF-β1. Gene.

Mi-Hwi Kima et. al. (December 2016). EX4 stabilizes and activates Nrf2 via PKCδ, contributing to the prevention of oxidative stress-induced pancreatic beta cell damage Science Direct. Toxicology and Applied Pharmacol

Read J et. al. (November 2016). Nuclear transportation of exogenous epidermal growth factor receptor and androgen receptor via extracellular vesicles. Eur J Cancer. 70:62-74.

Fujino T et. al. (November 2016). Alteration of histone H3K4 methylation in glomerular podocytes associated with proteinuria in patients with membranous nephropathy. BMC Nephrol. 17(1):179.

Hou S et. al. (October 2016). XAB2 functions in mitotic cell cycle progression via transcriptional regulation of CENPE. Cell Death Dis. 7(10):e2409.

Lee JH et. al. (August 2016). STAT3-induced WDR1 overexpression promotes breast cancer cell migration. Cell Signal.

Liu J et. al. (July 2016). Curcumin prevents adriamycin-induced nephropathy MCP-1 expression through blocking histone acetylation Int J Clin Exp Med. 9(7):12696-12704.

Qiao MX et. al. (July 2016). Regulation of DEK expression by AP-2α and methylation level of DEK promoter in hepatocellular carcinoma. Oncol Rep.

Gao Q et. al. (July 2016). A novel role of microRNA 17-5p in the modulation of circadian rhythm. Sci Rep. 6:30070.

Zhu S et. al. (June 2016). MiR-548an, transcriptionally downregulated by HIF-1α/HDAC1, suppresses tumorigenesis of pancreatic cancer by targeting vimentin expression. Mol Cancer Ther.

Wang P et. al. (June 2016). p-CREB-1 promotes hepatic fibrosis through the transactivation of transforming growth factor-β1 expression in rats. Int J Mol Med.

Angrisano T et. al. (June 2016). Cyclical DNA Methylation and Histone Changes Are Induced by LPS to Activate COX-2 in Human Intestinal Epithelial Cells. PLoS One. 11(6):e0156671.

Duan R et. al. (May 2016). Histone Acetylation Regulation in Sleep Deprivation-Induced Spatial Memory Impairment. Neurochem Res.

Takahashi T et. al. (April 2016). A potential contribution of antimicrobial peptide LL-37 to tissue fibrosis and vasculopathy in systemic sclerosis. Br J Dermatol.

Wang X et. al. (March 2016). ICP4-induced miR-101 attenuates HSV-1 replication. Sci Rep. 6:23205.

Ursini G et. al. (February 2016). BDNF rs6265 methylation and genotype interact on risk for schizophrenia. Epigenetics. :1-13.

Shi H et. al. (January 2016). The oncoprotein HBXIP up-regulates FGF4 through activating transcriptional factor Sp1 to promote the migration of breast cancer cells. Biochem Biophys Res Commun.

Li Y et. al. (January 2016). HBXIP and LSD1 Scaffolded by lncRNA Hotair Mediate Transcriptional Activation by c-Myc. Cancer Res. 76(2):293-304.

Zhuang C et. al. (January 2016). A double-negative feedback loop between EZH2 and miR-26a regulates tumor cell growth in hepatocellular carcinoma Int J Oncol.

Yamashita T et. al. (December 2015). A potential contribution of altered cathepsin L expression to the development of dermal fibrosis and vasculopathy in systemic sclerosis. Exp Dermatol.

Narita T et. al. (December 2015). Lower expression of activating transcription factors 3 and 4 correlates with shorter progression-free survival in multiple myeloma patients receiving bortezomib plus dexamethasone therapy. Blood Cancer J. 5:e373.

Maeng YS et. al. (November 2015). Histone methylation levels correlate with TGFBIp and extracellular matrix gene expression in normal and granular corneal dystrophy type 2 corneal fibroblasts. BMC Med Genomics. 8(1):74.

Saigusa R et. al. (September 2015). Fli1 deficiency contributes to the downregulation of endothelial protein C receptor in systemic sclerosis: a possible role in pro-thrombotic condition. Br J Dermatol.

Yu J et. al. (August 2015). Molecular hydrogen attenuates hypoxia/reoxygenation injury of intrahepatic cholangiocytes by activating Nrf2 expression. Toxicol Lett. 238(3):11-19.

Ichimura Y et. al. (August 2015). Progranulin overproduction due to Fli1 deficiency contributes to the resistance of dermal fibroblasts to tumor necrosis factor α in systemic sclerosis. Arthritis Rheumatol.

Li H et. al. (July 2015). The Oncoprotein HBXIP Modulates the Feedback Loop of MDM2/p53 to Enhance the Growth of Breast Cancer. J Biol Chem.

Lee JD et. al. (March 2015). Synergic chemoprevention with dietary carbohydrate restriction and supplementation of AMPK-activating phytochemicals: the role of SIRT1. Eur J Cancer Prev.

Takahashi T et. al. (March 2015). A possible contribution of lipocalin-2 to the development of dermal fibrosis, pulmonary vascular involvement, and renal dysfunction in systemic sclerosis. Br J Dermatol.

Arai T et. al. (March 2015). Translocation of forkhead box O1 to the nuclear periphery induces histone modifications that regulate transcriptional repression of PCK1 in HepG2 cells. Genes Cells.

Akamata K et. al. (February 2015). Endothelin receptor blockade ameliorates vascular fragility in endothelial cell-specific Fli1 knockout mice by increasing Fli1 DNA-binding ability. Arthritis Rheumatol.

Li J et. al. (February 2015). The Core Promoter and Redox-sensitive <i>Cis</i>-elements as Key Targets for Inactivation of the Lysyl Oxidase Gene by Cadmium. J Nat Sci. 1(2):e38.

Zhu M et. al. (January 2015). Alpha-fetoprotein activates AKT/mTOR signaling to promote CXCR4 expression and migration of hepatoma cells. Oncoscience. 2(1):59-70.

Tyagi E et. al. (January 2015). Interactive actions of Bdnf methylation and cell metabolism for building neural resilience under the influence of diet. Neurobiol Dis. 73:307-18.

Li Y et. al. (December 2014). The oncoprotein HBXIP enhances migration of breast cancer cells through increasing filopodia formation involving MEKK2/ERK1/2/Capn4 signaling. Cancer Lett. 355(2):288-96.

Cheng G et. al. (December 2014). NNK, a Tobacco-Specific Carcinogen, Inhibits the Expression of Lysyl Oxidase, a Tumor Suppressor. Int J Environ Res Public Health. 12(1):64-82.

Akamata K et. al. (December 2014). Increased expression of chemerin in endothelial cells due to Fli1 deficiency may contribute to the development of digital ulcers in systemic sclerosis. Rheumatology (Oxford).

Saigusa R et. al. (November 2014). A possible contribution of endothelial CCN1 downregulation due to Fli1 deficiency to the development of digital ulcers in systemic sclerosis. Exp Dermatol.

Leo E et. al. (November 2014). DNA Methyltransferase 1 Drives Transcriptional Down-Modulation of β Catenin Antagonist Chibby1 Associated With the BCR-ABL1 Gene of Chronic Myeloid Leukemia. J Cell Biochem.

Taniguchi T et. al. (November 2014). Fli1 haploinsufficiency induces fibrosis, vascular activation and immune abnormalities resembling systemic sclerosis in bleomycin-treated mice. Arthritis Rheumatol.

Bradley WD et. al. (November 2014). EZH2 Inhibitor Efficacy in Non-Hodgkin's Lymphoma Does Not Require Suppression of H3K27 Monomethylation. Chem Biol. 21(11):1463-1475.

Li J et. al. (October 2014). Sex-dependent regulation of hepatic CYP3A by growth hormone: Roles of HNF6, C/EBPα, and RXRα Biochem Pharmacol.

Ren ZJ et. al. (August 2014). Mir-509-5p joins the Mdm2/p53 feedback loop and regulates cancer cell growth. Cell Death Dis. 5:e1387.

Zheng LY et. al. (August 2014). Effect of angiotensin II on STAT3 mediated atrial structural remodeling. Eur Rev Med Pharmacol Sci. 18(16):2365-77.

Kudo H et. al. (June 2014). Decreased interleukin-20 expression in scleroderma skin contributes to cutaneous fibrosis. Arthritis Rheumatol. 66(6):1636-47.

Zhang W et. al. (May 2014). Hepatitis B virus X protein accelerates hepatocarcinogenesis with partner survivin through modulating miR-520b and HBXIP. Mol Cancer. 13(1):128.

Lu D et. al. (May 2014). Transcriptional control of ROS homeostasis by KUODA1 regulates cell expansion during leaf development. Nat Commun. 5:3767.

Ichimura Y et. al. (May 2014). Fli1 deficiency contributes to the suppression of endothelial CXCL5 expression in systemic sclerosis. Arch Dermatol Res. 306(4):331-8.

Sanders YY et. al. (May 2014). Histone deacetylase inhibition promotes fibroblast apoptosis and ameliorates pulmonary fibrosis in mice. Eur Respir J. 43(5):1448-58.

Zurlo D et. al. (April 2014). Cladosporol A, a new peroxisome proliferator-activated receptor γ (PPARγ) ligand, inhibits colorectal cancer cells proliferation through β-catenin/TCF pathway inactivation. Biochim Biophys Acta. 1840(7):2361-2372.

Du J et. al. (April 2014). Hydrogen sulfide suppresses oxidized low-density lipoprotein (ox-LDL)-stimulated monocyte chemoattractant protein 1 generation from macrophages via the nuclear factor κB (NF-κB) pathway. J Biol Chem. 289(14):9741-53.

Akamata K et. al. (April 2014). Bosentan reverses the pro-fibrotic phenotype of systemic sclerosis dermal fibroblasts via increasing DNA binding ability of transcription factor Fli1. Arthritis Res Ther. 16(2):R86.

Kuroda N et. al. (April 2014). Apoptotic response through a high mobility box 1 protein-dependent mechanism in LPS/GalN-induced mouse liver failure and glycyrrhizin-mediated inhibition. PLoS One. 9(4):e92884.

You X et. al. (January 2014). Hepatitis B virus X protein upregulates Lin28A/Lin28B through Sp-1/c-Myc to enhance the proliferation of hepatoma cells. Oncogene. 33(4):449-60.

Sui WG et. al. (January 2014). ChIP-seq analysis of histone H3K9 trimethylation in peripheral blood mononuclear cells of membranous nephropathy patients. Braz J Med Biol Res. 47(1):42-9.

Sato S et. al. (January 2014). A circadian clock gene, Rev-erbα, modulates the inflammatory function of macrophages through the negative regulation of Ccl2 expression. J Immunol. 192(1):407-17.

Hanzu FA et. al. (December 2013). Histone demethylase KDM1A represses inflammatory gene expression in preadipocytes. Obesity (Silver Spring). 21(12):E616-25.

Mellor P et. al. (December 2013). CREB3L1 is a metastasis suppressor that represses expression of genes regulating metastasis, invasion, and angiogenesis. Mol Cell Biol. 33(24):4985-95.

Piao J et. al. (October 2013). Sirt6 regulates postnatal growth plate differentiation and proliferation via Ihh signaling. Sci Rep. 3:3022.

Herpin A et. al. (October 2013). Divergent expression regulation of gonad development genes in medaka shows incomplete conservation of the downstream regulatory network of vertebrate sex determination. Mol Biol Evol. 30(10):2328-46.

Mancini M et. al. (September 2013). Chibby drives β catenin cytoplasmic accumulation leading to activation of the unfolded protein response in BCR-ABL1+ cells. Cell Signal. 25(9):1820-7.

Matsumoto T et. al. (June 2013). BMP-2 Induced Expression of Alx3 That Is a Positive Regulator of Osteoblast Differentiation. PLoS One. 8(6):e68774.

Xu F et. al. (June 2013). The oncoprotein HBXIP up-regulates Skp2 via activating transcription factor E2F1 to promote proliferation of breast cancer cells. Cancer Lett. 333(1):124-32.

Zhang Y et. al. (May 2013). The oncoprotein HBXIP upregulates PDGFB via activating transcription factor Sp1 to promote the proliferation of breast cancer cells. Biochem Biophys Res Commun. 434(2):305-10.

Ejarque M et. al. (April 2013). Neurogenin3 cooperates with Foxa2 to autoactivate its own expression. J Biol Chem. 288(17):11705-17.

D'Amici S et. al. (April 2013). TNFα modulates Fibroblast Growth Factor Receptor 2 gene expression through the pRB/E2F1 pathway: identification of a non-canonical E2F binding motif. PLoS One. 8(4):e61491.

Mikami N et. al. (April 2013). Calcitonin gene-related peptide and cyclic adenosine 5'-monophosphate/protein kinase A pathway promote IL-9 production in Th9 differentiation process. J Immunol. 190(8):4046-55.

Gao S et. al. (April 2013). Hypoxia-response element (HRE)-directed transcriptional regulation of the rat lysyl oxidase gene in response to cobalt and cadmium. Toxicol Sci. 132(2):379-89.

Yue L et. al. (April 2013). The oncoprotein HBXIP activates transcriptional coregulatory protein LMO4 via Sp1 to promote proliferation of breast cancer cells. Carcinogenesis. 34(4):927-35.

Algamas-Dimantov A et. al. (April 2013). Epigenetic control of HNF-4α in colon carcinoma cells affects MUC4 expression and malignancy. Cell Oncol (Dordr). 36(2):155-67.

Siles L et. al. (April 2013). ZEB1 imposes a temporary stage-dependent inhibition of muscle gene expression and differentiation via CtBP-mediated transcriptional repression. Mol Cell Biol. 33(7):1368-82.

Latrasse D et. al. (March 2013). Dual function of MIPS1 as a metabolic enzyme and transcriptional regulator. Nucleic Acids Res. 41(5):2907-17.

Chiarella S et. al. (March 2013). Nucleophosmin mutations alter its nucleolar localization by impairing G-quadruplex binding at ribosomal DNA. Nucleic Acids Res. 41(5):3228-39.

Sanders YY et. al. (January 2013). Histone modifications in senescence-associated resistance to apoptosis by oxidative stress. Redox Biol. 1(1):8-16.

Pelascini LP et. al. (January 2013). Histone deacetylase inhibition activates transgene expression from integration-defective lentiviral vectors in dividing and non-dividing cells. Hum Gene Ther. 24(1):78-96.

Noda S et. al. (November 2012). Constitutive activation of c-Abl/protein kinase C-δ/Fli1 pathway in dermal fibroblasts derived from patients with localized scleroderma. Br J Dermatol. 167(5):1098-105.

Mikami N et. al. (November 2012). Calcitonin gene-related peptide enhances experimental autoimmune encephalomyelitis by promoting Th17-cell functions. Int Immunol. 24(11):681-91.

Liu S et. al. (August 2012). The oncoprotein HBXIP uses two pathways to up-regulate S100A4 in promotion of growth and migration of breast cancer cells. J Biol Chem. 287(36):30228-39.

Mancini M et. al. (August 2012). Gadd45a transcriptional induction elicited by the Aurora kinase inhibitor MK-0457 in Bcr-Abl-expressing cells is driven by Oct-1 transcription factor. Leuk Res. 36(8):1028-34.

Mancini M et. al. (August 2012). Cytoplasmatic compartmentalization by Bcr-Abl promotes TET2 loss-of-function in chronic myeloid leukemia. J Cell Biochem. 113(8):2765-74.

Du Y et. al. (July 2012). Elevation of highly up-regulated in liver cancer (HULC) by hepatitis B virus X protein promotes hepatoma cell proliferation via down-regulating p18. J Biol Chem. 287(31):26302-11.

Morillo SM et. al. (July 2012). Nerve growth factor-induced cell cycle reentry in newborn neurons is triggered by p38MAPK-dependent E2F4 phosphorylation. Mol Cell Biol. 32(14):2722-37.

Fan-xin M et. al. (June 2012). Heat shock factor 1 regulates the expression of the TRPV1 gene in the rat preoptic-anterior hypothalamus area during lipopolysaccharide-induced fever. Exp Physiol. 97(6):730-40.

Mahan AL et. al. (March 2012). Epigenetic modulation of Homer1a transcription regulation in amygdala and hippocampus with pavlovian fear conditioning. J Neurosci. 32(13):4651-9.

Yamamura S et. al. (February 2012). MicroRNA-34a suppresses malignant transformation by targeting c-Myc transcriptional complexes in human renal cell carcinoma. Carcinogenesis. 33(2):294-300.

Yamamura S et. al. (January 2012). MicroRNA-34a modulates c-Myc transcriptional complexes to suppress malignancy in human prostate cancer cells. PLoS One. 7(1):e29722.

Endo T et. al. (December 2011). Thyroid-specific gene expression in chondrocytes. Biochem Biophys Res Commun. 416(3-4):227-31.

Pasha Z et. al. (August 2011). Efficient non-viral reprogramming of myoblasts to stemness with a single small molecule to generate cardiac progenitor cells. PLoS One. 6(8):e23667.

Li W et. al. (August 2011). DNA methylation and histone modifications regulate de novo shoot regeneration in Arabidopsis by modulating WUSCHEL expression and auxin signaling. PLoS Genet. 7(8):e1002243.

Cho YH et. al. (July 2011). Identification of transcriptional regulatory elements required for the Mup2 expression in circadian clock mutant mice. Biochem Biophys Res Commun. 410(4):834-40.

Nocchi L et. al. (June 2011). Thrombomodulin is silenced in malignant mesothelioma by a poly(ADP-ribose) polymerase-1-mediated epigenetic mechanism. J Biol Chem. 286(22):19478-88.

Kashif M et. al. (June 2011). p45NF-E2 represses Gcm1 in trophoblast cells to regulate syncytium formation, placental vascularization and embryonic growth. Development. 138(11):2235-47.

Kazumori H et. al. (May 2011). Roles of Kruppel-like factor 4 in oesophageal epithelial cells in Barrett's epithelium development. Gut. 60(5):608-17.

Hu N et. al. (April 2011). miR-520b regulates migration of breast cancer cells by targeting hepatitis B X-interacting protein and interleukin-8. J Biol Chem. 286(15):13714-22.

Garaud S et. al. (April 2011). CD5 promotes IL-10 production in chronic lymphocytic leukemia B cells through STAT3 and NFAT2 activation. J Immunol. 186(8):4835-44.

Yamada N et. al. (November 2010). Expression of MUC5AC, an early marker of pancreatobiliary cancer, is regulated by DNA methylation in the distal promoter region in cancer cells. J Hepatobiliary Pancreat Sci. 17(6):844-54.

Subkhankulova T et. al. (October 2010). Bmi1 directly represses p21Waf1/Cip1 in Shh-induced proliferation of cerebellar granule cell progenitors. Mol Cell Neurosci. 45(2):151-62.

Kavak E et. al. (October 2010). Analysis of the Wnt/B-catenin/TCF4 pathway using SAGE, genome-wide microarray and promoter analysis: Identification of BRI3 and HSF2 as novel targets. Cell Signal. 22(10):1523-35.

Musri MM et. al. (September 2010). Histone demethylase LSD1 regulates adipogenesis. J Biol Chem. 285(39):30034-41.

Aoki T et. al. (September 2010). Ets-1 promotes the progression of cerebral aneurysm by inducing the expression of MCP-1 in vascular smooth muscle cells. Gene Ther. 17(9):1117-23.

Chung S et. al. (August 2010). Glutaredoxin 1 regulates cigarette smoke-mediated lung inflammation through differential modulation of I{kappa}B kinases in mice: impact on histone acetylation. Am J Physiol Lung Cell Mol Physiol. 299(2):L192-203.

Yamaguchi T et. al. (August 2010). Cortisol is involved in temperature-dependent sex determination in the Japanese flounder. Endocrinology. 151(8):3900-8.

Hu B et. al. (July 2010). Epigenetic regulation of myofibroblast differentiation by DNA methylation. Am J Pathol. 177(1):21-8.

Sánchez-Tilló E et. al. (June 2010). ZEB1 represses E-cadherin and induces an EMT by recruiting the SWI/SNF chromatin-remodeling protein BRG1. Oncogene. 29(24):3490-500.

Dandrea M et. al. (November 2009). MeCP2/H3meK9 are involved in IL-6 gene silencing in pancreatic adenocarcinoma cell lines. Nucleic Acids Res. 37(20):6681-90.

Bianconcini A et. al. (November 2009). Transcriptional activity of the murine retinol-binding protein gene is regulated by a multiprotein complex containing HMGA1, p54 nrb/NonO, protein-associated splicing factor (PSF) and steroidogenic factor 1 (SF1)/liver receptor homologue 1 (LRH-1). Int J Biochem Cell Biol. 41(11):2189-203.

Zhang L et. al. (September 2009). Thyrotropin receptor activation increases hyaluronan production in preadipocyte fibroblasts: contributory role in hyaluronan accumulation in thyroid dysfunction. J Biol Chem. 284(39):26447-55.

Garaud S et. al. (May 2009). IL-6 modulates CD5 expression in B cells from patients with lupus by regulating DNA methylation. J Immunol. 182(9):5623-32.

Romanelli MG et. al. (March 2009). Characterization and functional analysis of cis-acting elements of the human farnesyl diphosphate synthetase (FDPS) gene 5' flanking region. Genomics. 93(3):227-34.

Fabre S et. al. (September 2008). FOXO1 regulates L-Selectin and a network of human T cell homing molecules downstream of phosphatidylinositol 3-kinase. J Immunol. 181(5):2980-9.

Mora-López F et. al. (August 2008). Transcription of PRDM1, the master regulator for plasma cell differentiation, depends on an SP1/SP3/EGR-1 GC-box. Eur J Immunol. 38(8):2316-24.

Cheetham S et. al. (June 2008). SPARC promoter hypermethylation in colorectal cancers can be reversed by 5-Aza-2'deoxycytidine to increase SPARC expression and improve therapy response. Br J Cancer. 98(11):1810-9.

Yamada N et. al. (April 2008). MUC1 expression is regulated by DNA methylation and histone H3 lysine 9 modification in cancer cells. Cancer Res. 68(8):2708-16.

Ullerås E et. al. (March 2008). NFAT but not NF-kappaB is critical for transcriptional induction of the prosurvival gene A1 after IgE receptor activation in mast cells. Blood. 111(6):3081-9.

Venteclef N et. al. (February 2008). Regulation of anti-atherogenic apolipoprotein M gene expression by the orphan nuclear receptor LRH-1. J Biol Chem. 283(7):3694-701.

Tsai CT et. al. (January 2008). Angiotensin II activates signal transducer and activators of transcription 3 via Rac1 in atrial myocytes and fibroblasts: implication for the therapeutic effect of statin in atrial structural remodeling. Circulation. 117(3):344-55.

Teng Y et. al. (November 2007). AS1411 alters the localization of a complex containing protein arginine methyltransferase 5 and nucleolin. Cancer Res. 67(21):10491-500.

Mora-López F et. al. (November 2007). Human BSAP and BLIMP1 conform an autoregulatory feedback loop. Blood. 110(9):3150-7.

Liu DX et. al. (November 2007). Transcriptional activation of p53 by Pitx1. Cell Death Differ. 14(11):1893-907.

Gao S et. al. (August 2007). Cloning and characterization of the rat lysyl oxidase gene promoter: identification of core promoter elements and functional nuclear factor I-binding sites. J Biol Chem. 282(35):25322-37.

Sakurai T et. al. (August 2007). Functional roles of Fli-1, a member of the Ets family of transcription factors, in human breast malignancy. Cancer Sci. 98(11):1775-84.

Venteclef N et. al. (February 2007). Interleukin-1 receptor antagonist induction as an additional mechanism for liver receptor homolog-1 to negatively regulate the hepatic acute phase response. J Biol Chem. 282(7):4393-9.

Customer Reviews

Rating by m*****@ucdenver.edu Verified Purchase Reviewed on: Tuesday 20 December, 2016
Application Description
The product I used is EpiQuick Chromatin Immunoprecipitation (ChiP) Kit (P-2002), and CtBP1 antibody (A-2705). I used this for a Chip assay to examine genes that bind with CtBP1 protein.

Pros: The antibody works good with human cells on CHiP assay. The ChiP kit is quick. Does not require huge amount of cells to start with.

Cons: The tiny tubes provided are kind of hard to work with. Not easy to label or to put in and get out of the heater we used.

Other Thoughts
The user guide should write more details

#1: Since the kit is shipped in two parts. One is stored at room temperature and the other is stored at 4C. I once accidentally stored all at room temperature for a couple of days. I called the technique support and asked if I can still use the part should be stored at 4C. I hope this can be written in the storage section.

The following suggestions are for people who has no previous experience with ChiP assay:

#2: The negative (normal mouse IgG) and positive control (Anti-RNA Polymerase II) are for the whole assay, not for each individual sample.

#3: When collecting cells, what temperature should people centrifuge the cells with? room temperature or 4C? I assume it is room temperature because other steps are done at room temperature.

#4: step 1 during cell lysis: since use ice cold PBS, so centrifuge also at 4C? after centrifuge, remove PBS

#5: step 2 during cell lysis: after votext 10second, centrifuge at 4C?

#6: last step of DNA shearing: what is the temperature of centrifuge? cross-linked DNA reversal/DNA purification procedure:

#7: step 3: add 150ul of CP7 to the samples and transfer to the columns. How about "input DNA"?

#8: step 4-7: should people do the same thing for the "input DNA" vials?

I hope I described everything clearly. In general, please treat user as beginners and write as much details as possible. If you can, also write down the purpose of each step.
20% Off m6A Antibodies
Related Products

Researchers Also Purchased
EpiQuik Tissue Chromatin Immunoprecipitation (ChIP) Kit
EpiQuik Tissue Chromatin Immunoprecipitation (ChIP) Kit
EpiQuik Nuclear Extraction Kit
EpiQuik Nuclear Extraction Kit
Histone H3K4me3 (H3K4 Trimethyl) Polyclonal Antibody
Histone H3K4me3 (H3K4 Trimethyl) Polyclonal Antibody
Methylamp Global DNA Methylation Quantification Ultra Kit
Methylamp Global DNA Methylation Quantification Ultra Kit
Terms & Conditions | Privacy Policy | Site Map
Copyright © 2019 EpiGentek Group Inc. All rights reserved.