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EpiQuik Tissue Chromatin Immunoprecipitation (ChIP) Kit


For immunoprecipitating chromatin specifically from tissue input samples via microplate format

Citations (42) | Write a Review
Suggested Workflow
Chromatin Isolation
Chromatin Shearing
PCR Analysis
Schematic procedure of the EpiQuik™ Tissue Chromatin Immunoprecipitation (ChIP) Kit.Fig. 2. Comparative overview of the EpiQuik™ Tissue Chromatin Immunoprecipitation (ChIP) Kit.
Input Type: Chromatin
Research Area: Chromatin & Transcription
Target Application: Immunoprecipitation
Vessel Format: 96-Well Plate
100% Guarantee: 6 months
Catalog No.SizePriceQty
P-2003-124 reactions $249.00 
P-2003-248 reactions $399.00 
P-2003-396 reactions $589.00 
Availability: Usually Ships in 1 Day or Same day delivery Same Day NY Delivery 
Product Overview

The EpiQuik™ Tissue Chromatin Immunoprecipitation (ChIP) Kit is a convenient package of tools that allows the experimenter to quickly perform chromatin immunoprecipitation (ChIP) from mammalian tissue. The kit is ready-to-use and provides all the essential components needed to carry out a successful ChIP experiment on tissue. The EpiQuik™ ChIP kits are suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, ChIP-Seq, and ChIP-on-chip. This kit has the following advantages:

  • The fastest procedure available, which can be finished within 5 hours.
  • The ChIP procedure has been drastically simplified -- extremely short and easy to follow.
  • Strip microplate format makes the assay flexible: manual or high throughput.
  • Columns for DNA purification are included, which save time and reduce labor.
  • Compatible with all DNA amplification-based approaches.
  • Achieves very reliable and consistent assay conditions.

See also a quick chart to compare ChIP kits.

Background Information
Protein-DNA interaction play a critical role for cellular functions such as signal transduction, gene transcription, chromosome segregation, DNA replication and recombination, and epigenetic silencing. Identifying the genetic targets of DNA binding proteins and knowing the mechanisms of protein-DNA interaction is important for understanding cellular process. Chromatin Immunoprecipitation (ChIP) offers an advantageous tool for studying protein-DNA interactions. Unlike other methods such as EMASA, DNA microarrays, and report gene assays, which analyze direct interactions between protein and DNA in vitro, ChIP can detect that a specific protein binds to the specific sequences of a gene in living cells.   

Principle & Procedure
This ChIP kit includes all reagents required for carrying out a successful chromatin immunoprecipitation from mammalian tissue. Particularly, this kit includes a positive control antibody (RNA polymerase II), a negative control normal mouse IgG, and GAPDH primers that can be used as a positive control to demonstrate the efficacy of the kit reagents and protocol. RNA polymerase II is considered to be enriched in the GAPDH gene promoter that is expected to be undergoing transcription in most growing mammalian cells and can be immunoprecipitated by RNA polymerase II but not by normal mouse IgG. In this ChIP, cells are cross-linked with formaldehyde and chromatin is extracted. The chromatin is then sheared and added into the microwell immobilized with affinity antibodies. Cross-linked DNA is released from antibody-captured protein-DNA complex, reversed and purified through the specifically designed F-Spin Column. Eluted DNA can be used for various down-stream applications. 

Starting Materials
Starting materials can include various tissue samples. In general, the input amount should be from 10 to 30 mg of tissue for each reaction.

Fig. 1. Schematic procedure of the EpiQuik™ Tissue Chromatin Immunoprecipitation (ChIP) Kit.

Fig. 2. Comparative overview of the EpiQuik™ Tissue Chromatin Immunoprecipitation (ChIP) Kit.

Product Components

CP1 (Wash Buffer)
CP2 (Antibody Buffer)
CP3 (Lysis Buffer)
CP4 (ChIP Dilution Buffer)
CP5 (DNA Release Buffer)
CP6 (Reverse Buffer)
CP7 (Binding Buffer)
CP8 (Elution Buffer)
Homogenizing Buffer
Protease Inhibitor Cocktail (100X)*
Normal Mouse IgG (1 mg/ml)*
Anti-RNA Polymerase II (1 mg/ml)*
Proteinase K (10 mg/ml)*
Control Primer (GAPDH)
Forward (20 µM)*
Reverse (20 µM)*
8-Well Assay Strips (with Frame)
8-Well Strip Caps
F-Spin Column
F-Collection Tube
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

Frequently Asked Q's

1. Can IP be overnight?

2. Is there any background DNA by mouse IgG IP?
Yes, a little < 2 ng.

3. Can any species Ab be used for the IP?
Yes, if it is an IgG antibody.

4. Can kit P-2003 be used for frozen tissues?
Yes, but it is not as good as fresh samples.

5. How much volume of elution should be added into PCR reaction?
2 µl.

6. Are cell extracts containing 1%SDS compatible with CP4?
No, the SDS concentration in IP solution should be less than 0.1%.

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing info@epigentek.com along with your contact information and institution name.

[Safety Data Sheet]
Product Citations

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Heesch MW et. al. (July 2016). Transcriptional control, but not sub-cellular location, of PGC-1α is altered following exercise in a hot environment. J Appl Physiol (1985). :jap.01065.2015.

Chater-Diehl EJ et. al. (May 2016). Alteration of Gene Expression, DNA Methylation, and Histone Methylation in Free Radical Scavenging Networks in Adult Mouse Hippocampus following Fetal Alcohol Exposure. PLoS One. 11(5):e0154836.

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Wang Y et. al. (January 2015). Novel curcumin analog C66 prevents diabetic nephropathy via JNK pathway with the involvement of p300/CBP-mediated histone acetylation. Biochim Biophys Acta. 1852(1):34-46.

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Zhang W et. al. (May 2014). Prenatal alcohol exposure causes the over-expression of DHAND and EHAND by increasing histone H3K14 acetylation in C57 BL/6 mice. Toxicol Lett. 228(3):140-146.

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Monteiro JB et. al. (March 2014). Endometriosis is characterized by a distinct pattern of histone 3 and histone 4 lysine modifications. Reprod Sci. 21(3):305-18.

Anier K et. al. (October 2013). S-adenosylmethionine modifies cocaine-induced DNA methylation and increases locomotor sensitization in mice. Int J Neuropsychopharmacol. 16(9):2053-66.

Das B et. al. (May 2013). The sld genetic defect: two intronic CA repeats promote insertion of the subsequent intron and mRNA decay. J Biol Chem. 288(21):14742-55.

Fan-xin M et. al. (June 2012). Heat shock factor 1 regulates the expression of the TRPV1 gene in the rat preoptic-anterior hypothalamus area during lipopolysaccharide-induced fever. Exp Physiol. 97(6):730-40.

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Wu CH et. al. (November 2011). A double-edged sword role for ubiquitin-proteasome system in brain stem cardiovascular regulation during experimental brain death. PLoS One. 6(11):e27404.

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Aoki T et. al. (September 2010). Ets-1 promotes the progression of cerebral aneurysm by inducing the expression of MCP-1 in vascular smooth muscle cells. Gene Ther. 17(9):1117-23.

Chung S et. al. (August 2010). Glutaredoxin 1 regulates cigarette smoke-mediated lung inflammation through differential modulation of I{kappa}B kinases in mice: impact on histone acetylation. Am J Physiol Lung Cell Mol Physiol. 299(2):L192-203.

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