EpiSonic 2000 Sonication System

The EpiSonic™ 2000 Sonication System is the latest sonication-based instrument with integrated cooling for DNA shearing and chromatin shearing applications. This sonicator is designed for simultaneous, optimized processing of 1 to 12 samples at small microliter-level volumes (optimal at 15 µl of volume), making it an ideal solution for next-generation sequencing due to its high shearing consistency and uniformity.

Features At-A-Glance
The EpiSonic™ 2000 incorporates Digitally Adaptive Sonocavitation™ (DAS) technology to efficiently process DNA and chromatin samples, with the following key features:

• Simple input and controls
• Simultaneous sonication of up to 12 samples
• 10 minutes to shear 12 samples to 300 bp (50 sec/sample)
• Reproducible and consistent fragments
• Closed vessel processing
• Easy installation and maintenance
• Continuous cooling system
• 20-month warranty

Key Points
Notable points of the EpiSonic™ 2000 include the following:

• Generates fragments compatible with Illumina, Ion Torrent, 454, and Pacific Biosciences platforms.
• Generates tight and highly reproducible fragment distributions.
• Capable of achieving uniform fragment sizes between 150 bps and 20 kbs.
• Meets "the scientist's requirements" for a low-cost, flexible, higher throughput and easy-to-use design.
• Eliminates operator-induced variations, improves recovery yield, increases efficiency, and provides results that can be standardized.

Fig. 2. Demonstration of consistent fragments achieved with a single run of 12-sample multiplex shearing with the EpiSonic™ 2000.

DAS™ technology, incorporated into the EpiSonic™ 2000, provides the following advantages:

Fig. 1. Setup of the EpiSonic™ 2000 Sonication System. 

Specifications

Reproducibility at 2 kb shearing

Reproducibility at 6 kb shearing

Reproducibility at 20 kb shearing