The discovery of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and Cas9 (CRISPR associated system or CRISPR associated protein 9 nuclease) found in bacteria to work as a defense mechanism against foreign DNA, has proven to be an invaluable tool to target and modify a genetic sequence in gene editing and genome engineering applications. The system, known as CRISPR/Cas9, allows for sequence-specific cleavage of a targeted genomic locus by delivering the RNA-guided nuclease (Cas9) and appropriate guide RNAs (gRNA) into a cell. In addition, Protospacer Adjacent Motif (PAM) sequence immediately following the specificity sequence is necessary for successful binding of the Cas9 nuclease.
Mouse monoclonal antibody raised against CRISPR/Cas9, clone 7A9, generated with synthesized peptide corresponding to sequence of Streptococcus pyogene (S. pyogenes) CRISPR-associated endonuclease Cas9/Csn1. This Anti-Cas9 mAb can detect CRISPR/Cas9 expression in target cells by WB, IF, IP, or ELISA to confirm and verify whether gRNA and Cas9 vectors are successfully transfected.
Recognizes both Cas9 and dCas9 (nuclease deficient Cas9)
Recombinant N-terminal fragment of S. pyogenes Cas9 protein expressed in E. coli.
PBS, 30% Glycerol
Store at 4°C for short term (1-2 weeks). For long term storage, aliquot and then store at −20°C . Avoid multiple freeze/thaw cycles.
Protein G Purified
Anti-Cas9, Anti-CRISPR, Anti-CRISPR/Cas9, CRISPR antibody, Cas9 antibody, Cas9 7A9, 7A9-3A3
WB (1:500), IP (2 µg/10^6 cells), IF (1:500), ELISA (1:1000-1:2000)