We recommend our monoclonal Anti-Cas9-CRISPR (Cat #A-9000).
The discovery of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and Cas9 (CRISPR associated system or CRISPR associated protein 9 nuclease) found in bacteria to work as a defense mechanism against foreign DNA, has proven to be an invaluable tool to target and modify a genetic sequence in gene editing and genome engineering applications. The system, known as CRISPR/Cas9, allows for sequence-specific cleavage of a targeted genomic locus by delivering the RNA-guided nuclease (Cas9) and appropriate guide RNAs (gRNA) into a cell. In addition, Protospacer Adjacent Motif (PAM) sequence immediately following the specificity sequence is necessary for successful binding of the Cas9 nuclease.
Rabbit polyclonal antibody to CRISPR/Cas9, generated with synthesized peptide corresponding to sequence of Streptococcus pyogene (S. pyogenes) CRISPR-associated endonuclease Cas9/Csn1, with further optimization for IP and ChIP due to recognition of multiple epitopes. This antibody can (a) detect CRISPR/Cas9 expression in target cells by WB, IF, or ELISA to confirm and verify whether gRNA and Cas9 vectors are successfully transfected; or (b) detect genome-wide off- and on-target effects of CRISPR/Cas9 on specific gene sites by Cas9 ChIP-seq in CRISPR genome editing.
Recognizes both Cas9 and dCas9 (nuclease deficient Cas9)
Liquid containing 0.05% Sodium Azide, 1X TBS (pH7.4), 0.5% BSA, 40% Glycerol
Store at 4°C for 3 weeks. For long term storage, aliquot and then store at −20°C or −80°C. Avoid multiple freeze/thaw cycles.
Protein A purified
For maximum recovery of the products, centrifuge the vial prior to opening the cap
Anti-Cas9, Anti-CRISPR, Anti-CRISPR/Cas9, CRISPR antibody, Cas9 antibody
WB: (1:2000 - 1:5000), ELISA: (1:5000-1:10000), ChIP or IP: (2 µg/106 cells), IF: (1:200-1:500), ICC: (1:200-1:500)