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CRISPR/Cas9 Polyclonal Antibody


For antibody-based studies in gene editing and genome engineering

Citations (2) | (1) | Write a Review
Western blot analysis on Cas9 transfected 293 cells (+) and un-transfected 293 cells (-).
Specific detection of Cas9 nuclease by CRISPR/Cas9 pAb (Cat. #A-1111) via ELISA. Cas9 nuclease was added into the assay wells and detected with CRISPR/Cas9 pAb (at 1:1000 dilution).
Successful immunoprecipitation of Cas9 nuclease by CRISPR/Cas9 pAb (Cat. #A-1111). 200 ng of nuclease was immunoprecipitated with 0.5 ug of CRISPR/Cas9 pAb. Rabbit non-immune IgG was used as control.
Immunofluorescence analysis of CRISPR/Cas9 transfected 293 cells. The cell expressing CRISPR/Cas9 can be detected using rabbit anti-CRISPR/Cas9 (1:500) followed by FITC (1:1000) goat anti-rabbit IgG.
Areas of Research: Other Primary Antibodies
Clonality: Other Antibodies
Conjugate: Unconjugated
Host: Rabbit
Isotype: IgG
100% Guarantee: 6 months
Catalog No.SizePriceQty
A-1111-01010 µg $71.00  Discontinued
A-1111-05050 µg $195.00  Discontinued
A-1111-100100 µg $349.00  Discontinued
Availability: Discontinued 
Product Overview

We recommend our monoclonal Anti-Cas9-CRISPR (Cat #A-9000).

The discovery of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and Cas9 (CRISPR associated system or CRISPR associated protein 9 nuclease) found in bacteria to work as a defense mechanism against foreign DNA, has proven to be an invaluable tool to target and modify a genetic sequence in gene editing and genome engineering applications. The system, known as CRISPR/Cas9, allows for sequence-specific cleavage of a targeted genomic locus by delivering the RNA-guided nuclease (Cas9) and appropriate guide RNAs (gRNA) into a cell. In addition, Protospacer Adjacent Motif (PAM) sequence immediately following the specificity sequence is necessary for successful binding of the Cas9 nuclease.

1 mg/ml

Rabbit polyclonal antibody to CRISPR/Cas9, generated with synthesized peptide corresponding to sequence of Streptococcus pyogene (S. pyogenes) CRISPR-associated endonuclease Cas9/Csn1, with further optimization for IP and ChIP due to recognition of multiple epitopes. This antibody can (a) detect CRISPR/Cas9 expression in target cells by WB, IF, or ELISA to confirm and verify whether gRNA and Cas9 vectors are successfully transfected; or (b) detect genome-wide off- and on-target effects of CRISPR/Cas9 on specific gene sites by Cas9 ChIP-seq in CRISPR genome editing.

Recognizes both Cas9 and dCas9 (nuclease deficient Cas9)

Species Independent


Liquid containing 0.05% Sodium Azide, 1X TBS (pH7.4), 0.5% BSA, 40% Glycerol

Store at 4°C for 3 weeks. For long term storage, aliquot and then store at −20°C or −80°C. Avoid multiple freeze/thaw cycles.

Protein A purified

Handling Recommendations 
For maximum recovery of the products, centrifuge the vial prior to opening the cap

Alternative Names
Anti-Cas9, Anti-CRISPR, Anti-CRISPR/Cas9, CRISPR antibody, Cas9 antibody

WB: (1:2000 - 1:5000), ELISA: (1:5000-1:10000), ChIP or IP: (2 µg/106 cells), IF: (1:200-1:500), ICC: (1:200-1:500) 

User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by emailing along with your contact information and institution name.

Product Citations

Kleinjan DA et. al. (October 2017). Drug-tunable multidimensional synthetic gene control using inducible degron-tagged dCas9 effectors. Nat Commun. 8(1):1191.

Yao L et. al. (December 2015). Multiple Gene Repression in Cyanobacteria Using CRISPRi. ACS Synth Biol.

Customer Reviews

Rating by r*** Verified Purchase Reviewed on: Monday 27 April, 2015
Application Description
I’ve tested the polyclonal Cas9 antibody. Results are attached

Procedural Details
K562 cells were nucleofected with pX330-U6-Chimeric_BB-CBh-hSpCas9 (Addgene plasmid #42230) and protein was harvested 24 hrs after nucleofections. Protein samples equivalent of approx. 25,000 cells was added 4x Laemmli Sample Buffer containing 1/10 DTT. Samples were boiled for 5 min, centrifuged at 16,300xg for 30s, and loaded onto an ‘Any kD Mini-PROTEAN TGX’ precast polyacrylamide gel (Bio-Rad), which was run at 43 mA for approx. 30 min. Protein was transferred to a PVDF membrane using the Trans-Blot Turbo Transfer System (Bio-rad) using the Bio-Rad program ‘HIGH MW’. After blotting, the membrane was washed once in TBS-T and blocked for 1 hr in TBS-T with 5% milk. The membrane was then washed three times in TBS-T and incubated overnight at 4 °C with primary antibody (cat. # A-1111) diluted 1:5,000 in TBS-T w. 0.5% milk. The membrane was then washed three times in TBS-T and incubated with secondary antibody (anti-rabbit HRP, Santa Cruz Biotechnology cat. # sc-2004) at 1:10,000 in TBS-T with 0.5% milk for 1 hr at room temperature. The blot was then washed five times in TBS-T and incubated with 2mL of ECL substrate (Bio-Rad, Clarity ECL Western Blotting Substrate) at room temperature for 5 min. The blot was visualized using the ChemiDoc XRS+ System (Bio-Rad)

Cas9 pAb western blot
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