HRP-Goat Anti-Rabbit Secondary Antibody

Description
Goat anti-rabbit IgG recognizes rabbit IgG whole molecule. This secondary antibody was purified using antigen affinity chromatography. The antibody is conjugated with peroxidase.

Antibody Type
Polyclonal Antibody

Purification
Liquid; This product was prepared from monospecific antiserum by immunoaffinity chromatography, followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen
Rabbit IgG whole molecule

Isotype
IgG

Size
350 µg

Formulation
In 10 mM sodium phosphate, 75 mM NaCl, 50% (v/v) glycerol, pH 7.2 , 10 mg/mL BSA (Immunoglobulin and Protease free)

Specificity
Rabbit

Storage
Store at -20°C. Aliquot to avoid repeated freezing and thawing.

Handling Recommendations
The optimal working dilution should be determined by the end user. For maximum recovery of the products, centrifuge the vial prior to opening the cap. 

Applications & Suggested Dilutions
Western Blot: 1:1000-1: 10000;  Immunohistochemistry:1:100-1:500; Immunofluorescence: 1:100-1:500; ELISA: 1:2000-1:20000

 

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Hu P et. al. (January 2019). Effect of endostatin on proliferation, invasion and epithelial-mesenchymal transition of basal cell carcinoma cell A431. Eur Rev Med Pharmacol Sci. 23(2):877-884.