Background
DNMT3B has been demonstrated to methylate both unmethylated and hemimethylated DNA equally and is supposed to mediate de novo methylation together with Dnmt1. Increased activation or amount of Dnmt3b is believed to be involved in carcinogenesis, as well as other genetic and epigenetic diseases.

Description
DNMT3B Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Specificity
Human, Mouse

Isotype
IgG

Uniprot ID
Q9UBC3

Purification
Affinity Purified

Immunogen
Synthetic Peptide of Human DNMT3B

Storage
Shipped at 4°C. Store at -20°C. Avoid multiple freeze/thaw cycles.

Alternative Names
Cytosine 5methyltransferase 3B antibody, DNA (cytosine 5) methyltransferase 3 beta antibody, DNA (cytosine-5)-methyltransferase 3B antibody, DNA antibody, DNA methyltransferase HsaIIIB antibody, DNA MTase HsaIIIB antibody, DNM3B_HUMAN antibody, Dnmt3b antibody, EC 2.1.1.37 antibody, ICF antibody, ICF1 antibody, M.HsaIIIB antibody, MGC124407 antibody, RP23-89H14.3 antibody

Application
WB, IF; Recommended dilution: WB 1:200 - 1:1000; IF 1:50 - 1:200

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Qiao X et. al. (June 2017). 5-Aza-2'-deoxycytidine in the medial prefrontal cortex regulates alcohol-related behavior and Ntf3-TrkC expression in rats. PLoS One. 12(6):e0179469.

Zhang Q et. al. (October 2016). Rhein reversal of DNA hypermethylation-associated Klotho suppression ameliorates renal fibrosis in mice. Sci Rep. 6:34597.

Vottero A et. al. (December 2011). Evidence for epigenetic abnormalities of the androgen receptor gene in foreskin from children with hypospadias. J Clin Endocrinol Metab. 96(12):E1953-62.

Socha MJ et. al. (February 2009). Aberrant promoter methylation of SPARC in ovarian cancer. Neoplasia. 11(2):126-35.

Kameda T et. al. (November 2006). A severe de novo methylation of episomal vectors by human ES cells. Biochem Biophys Res Commun. 349(4):1269-77.