DNMT1 Polyclonal Antibody

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Western blot analysis of extracts of various cell lines, using DNMT1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Western blot analysis of extracts from normal (control) and DNMT1 knockout (KO) 293T cells, using DNMT1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence analysis of 293T cells using DNMT1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug DNMT1 antibody. Western blot was performed from the immunoprecipitate using DNMT1 antibody at a dilition of 1:1000.

Application:	IF, IP, WB
Areas of Research:	DNA Methylation
Clonality:	Polyclonal
Conjugate:	Unconjugated
Host:	Rabbit
Isotype:	IgG
Purification:	Affinity Purified
Reactivity:	Human, Rat
100% Guarantee:	6 months

Catalog No.	Size	Price	Qty
A-1700-050	50 µl	$189.00
A-1700-100	100 µl	$299.00

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Product Overview

Background
DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene.

Description
DNMT1 Polyclonal Antibody. Unconjugated. Raised in: Rabbit.

Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Specificity
Human, Rat

Isotype
IgG

Uniprot ID
P26358

Purification
Affinity Purified

Immunogen
Recombinant Protein of Human DNMT1

Storage
Shipped at 4°C. Store at -20°C. Avoid multiple freeze/thaw cycles.

Alternative Names
AIM, DNMT, MCMT, CXXC9, HSN1E, ADCADN

Application
WB, IF, IP ; Recommended dilution: WB 1:500 - 1:2000; IF 1:50 - 1:200; IP 1:50 - 1:100

User Guide & MSDS

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